Dissecting Independent Channel and Scaffolding Roles of the Drosophila Transient Receptor Potential Channel
Drosophila transient receptor potential (TRP) serves dual roles as a cation channel and as a molecular anchor for the PDZ protein, INAD (inactivation no afterpotential D). Null mutations in trp cause impairment of visual transduction, mislocalization of INAD, and retinal degeneration. However, the impact of specifically altering TRP channel function is not known because existing loss-of-function alleles greatly reduce protein expression. In the current study we describe the isolation of a set of new trp alleles, including <tex-math>$trp^{14}$</tex-math> with an amino acid substitution juxtaposed to the TRP domain. The <tex-math>$trp^{14}$</tex-math> flies stably express TRP and display normal molecular anchoring, but defective channel function. Elimination of the anchoring function alone in <tex-math>$trp^{\Delta 1272}$</tex-math>, had minor effects on retinal morphology whereas disruption of channel function caused profound light-induced cell death. This retinal degeneration was greatly suppressed by elimination of the <tex-math>$Na^{+}/Ca^{2+}$</tex-math> exchanger, CalX, indicating that the cell death was due primarily to deficient Ca<sup>2+</sup> entry rather than disruption of the TRP-anchoring function..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2005 |
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| Erschienen: |
2005 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:171 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Tao [VerfasserIn] |
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| Links: |
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| Themen: |
Biological sciences |
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| PPN (Katalog-ID): |
JST045651256 |
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| 100 | 1 | |a Wang, Tao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Dissecting Independent Channel and Scaffolding Roles of the Drosophila Transient Receptor Potential Channel |
| 264 | 1 | |c 2005 | |
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| 520 | |a Drosophila transient receptor potential (TRP) serves dual roles as a cation channel and as a molecular anchor for the PDZ protein, INAD (inactivation no afterpotential D). Null mutations in trp cause impairment of visual transduction, mislocalization of INAD, and retinal degeneration. However, the impact of specifically altering TRP channel function is not known because existing loss-of-function alleles greatly reduce protein expression. In the current study we describe the isolation of a set of new trp alleles, including <tex-math>$trp^{14}$</tex-math> with an amino acid substitution juxtaposed to the TRP domain. The <tex-math>$trp^{14}$</tex-math> flies stably express TRP and display normal molecular anchoring, but defective channel function. Elimination of the anchoring function alone in <tex-math>$trp^{\Delta 1272}$</tex-math>, had minor effects on retinal morphology whereas disruption of channel function caused profound light-induced cell death. This retinal degeneration was greatly suppressed by elimination of the <tex-math>$Na^{+}/Ca^{2+}$</tex-math> exchanger, CalX, indicating that the cell death was due primarily to deficient Ca<sup>2+</sup> entry rather than disruption of the TRP-anchoring function. | ||
| 540 | |a Copyright 2005 The Rockefeller University Press | ||
| 650 | 4 | |a Health sciences |x Medical conditions |x Diseases |x Eye diseases |x Retinal diseases |x Retinal degeneration | |
| 650 | 4 | |a Biological sciences |x Biology |x Cytology |x Cell biology |x Cells |x Cellular receptors |x Sensory receptor cells |x Photoreceptors | |
| 650 | 4 | |a Biological sciences |x Biology |x Genetics |x Population genetics |x Genetic variation |x Genetic mutation | |
| 650 | 4 | |a Biological sciences |x Biology |x Zoology |x Animals |x Invertebrates |x Arthropods |x Insects |x Flies |x Fruit flies |x Drosophila | |
| 650 | 4 | |a Biological sciences |x Biology |x Genetics |x Phenotypes | |
| 650 | 4 | |a Biological sciences |x Biology |x Cytology |x Cell biology |x Cell physiology |x Cell death | |
| 650 | 4 | |a Biological sciences |x Biology |x Genetics |x Molecular genetics |x Genes |x Alleles | |
| 650 | 4 | |a Biological sciences |x Biochemistry |x Biomolecules |x Macromolecules |x Carbohydrates |x Glycoconjugates |x Glycoproteins |x Membrane glycoproteins |x Ion channels |x Transient receptor potential channels |x TRPC cation channels | |
| 650 | 4 | |a Health sciences |x Medical sciences |x Immunology |x Immune system |x Immune response |x Adaptive immunity |x Active immunity |x Humoral immunity |x Antibodies | |
| 650 | 4 | |a Physical sciences |x Chemistry |x Chemical compounds |x Chemicals |x Polymers |x Biopolymers |x Proteins |x Receptors | |
| 655 | 4 | |a research-article | |
| 700 | 1 | |a Jiao, Yuchen |e verfasserin |4 aut | |
| 700 | 1 | |a Montell, Craig |e verfasserin |4 aut | |
| 773 | 1 | 8 | |g volume:171 |g year:2005 |g number:4 |g pages:685-694 |
| 856 | 4 | 0 | |u https://www.jstor.org/stable/3658475 |3 Volltext |
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