Dipalmitoyl-phosphatidylserine-filled cationic maltodextrin nanoparticles exhibit enhanced efficacy for cell entry and intracellular protein delivery in phagocytic THP-1 cells
Abstract Vaccination through the upper respiratory tract is a promising strategy, and particulate antigens, such as antigens associated with nanoparticles, triggered a stronger immune response than the sole antigens. Cationic maltodextrin-based nanoparticles loaded with phosphatidylglycerol (NPPG) are efficient for intranasal vaccination but non-specific to trigger immune cells. Here we focused on phosphatidylserine (PS) receptors, specifically expressed by immune cells including macrophages, to improve nanoparticle targeting through an efferocytosis-like mechanism. Consequently, the lipids associated with NPPG have been substituted by PS to generate cationic maltodextrin-based nanoparticles with dipalmitoyl-phosphatidylserine (NPPS). Both NPPS and NPPG exhibited similar physical characteristics and intracellular distribution in THP-1 macrophages. NPPS cell entry was faster and higher (two times more) than NPPG. Surprisingly, competition of PS receptors with phospho-L-serine did not alter NPPS cell entry and annexin V did not preferentially interact with NPPS. Although the protein association is similar, NPPS delivered more proteins than NPPG in cells. On the contrary, the proportion of mobile nanoparticles (50%), the movement speed of nanoparticles (3 µm/5 min), and protein degradation kinetics in THP-1 were not affected by lipid substitution. Together, the results indicate that NPPS enter cells and deliver protein better than NPPG, suggesting that modification of the lipids of cationic maltodextrin-based nanoparticles may be a useful strategy to enhance nanoparticle efficacy for mucosal vaccination..
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
---|---|
Enthalten in: |
Biomolecular concepts - 14(2023), 1 vom: 28. Juni |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Brinkhuizen, Clément [VerfasserIn] |
---|
Links: |
Volltext [kostenfrei] |
---|
BKL: |
---|
Anmerkungen: |
© 2023 the author(s), published by De Gruyter |
---|
doi: |
10.1515/bmc-2022-0029 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
GRUY00899840X |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | GRUY00899840X | ||
003 | DE-627 | ||
005 | 20230813133023.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230813s2023 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1515/bmc-2022-0029 |2 doi | |
035 | |a (DE-627)GRUY00899840X | ||
035 | |a (DE-B1597)bmc-2022-0029-e | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 570 |q VZ |
082 | 0 | 4 | |a 570 |q VZ |
084 | |a 42.13 |2 bkl | ||
100 | 1 | |a Brinkhuizen, Clément |e verfasserin |4 aut | |
245 | 1 | 0 | |a Dipalmitoyl-phosphatidylserine-filled cationic maltodextrin nanoparticles exhibit enhanced efficacy for cell entry and intracellular protein delivery in phagocytic THP-1 cells |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
500 | |a © 2023 the author(s), published by De Gruyter | ||
520 | |a Abstract Vaccination through the upper respiratory tract is a promising strategy, and particulate antigens, such as antigens associated with nanoparticles, triggered a stronger immune response than the sole antigens. Cationic maltodextrin-based nanoparticles loaded with phosphatidylglycerol (NPPG) are efficient for intranasal vaccination but non-specific to trigger immune cells. Here we focused on phosphatidylserine (PS) receptors, specifically expressed by immune cells including macrophages, to improve nanoparticle targeting through an efferocytosis-like mechanism. Consequently, the lipids associated with NPPG have been substituted by PS to generate cationic maltodextrin-based nanoparticles with dipalmitoyl-phosphatidylserine (NPPS). Both NPPS and NPPG exhibited similar physical characteristics and intracellular distribution in THP-1 macrophages. NPPS cell entry was faster and higher (two times more) than NPPG. Surprisingly, competition of PS receptors with phospho-L-serine did not alter NPPS cell entry and annexin V did not preferentially interact with NPPS. Although the protein association is similar, NPPS delivered more proteins than NPPG in cells. On the contrary, the proportion of mobile nanoparticles (50%), the movement speed of nanoparticles (3 µm/5 min), and protein degradation kinetics in THP-1 were not affected by lipid substitution. Together, the results indicate that NPPS enter cells and deliver protein better than NPPG, suggesting that modification of the lipids of cationic maltodextrin-based nanoparticles may be a useful strategy to enhance nanoparticle efficacy for mucosal vaccination. | ||
700 | 1 | |a Shapman, Damien |4 aut | |
700 | 1 | |a Lebon, Alexis |4 aut | |
700 | 1 | |a Bénard, Magalie |4 aut | |
700 | 1 | |a Tardivel, Meryem |4 aut | |
700 | 1 | |a Dubuquoy, Laurent |4 aut | |
700 | 1 | |a Galas, Ludovic |4 aut | |
700 | 1 | |a Carpentier, Rodolphe |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Biomolecular concepts |d De Gruyter, 2010 |g 14(2023), 1 vom: 28. Juni |h Online-Ressource |w (DE-627)GRUY000022969 |w (DE-600)2558921-0 |w (DE-576)33012062X |x 1868-503X |7 nnns |
773 | 1 | 8 | |g volume:14 |g year:2023 |g number:1 |g day:28 |g month:06 |
856 | 4 | 0 | |u https://dx.doi.org/10.1515/bmc-2022-0029 |z kostenfrei |3 Volltext |
912 | |a GBV_GRUY | ||
912 | |a SSG-OLC-PHA | ||
936 | b | k | |a 42.13 |j Molekularbiologie |q VZ |
951 | |a AR | ||
952 | |d 14 |j 2023 |e 1 |b 28 |c 06 |