Biological coupling method of extracellular vesicles and alpha, beta-unsaturated carbonyl compounds
The invention discloses a biological coupling method of an extracellular vesicle and an alpha, beta-unsaturated carbonyl compound, which comprises the following steps: catalyzing primary amine on the surface of the extracellular vesicle and the alpha, beta-unsaturated carbonyl compound to generate Michael addition reaction through proper alkali, and finishing direct coupling of the extracellular vesicle and the alpha, beta-unsaturated carbonyl compound through a one-step method; a compound containing an alpha, beta-unsaturated carbonyl structure characteristic, such as a fluorescent tracer or a medicinal natural product, can be directly coupled to the extracellular vesicle through the method disclosed by the invention, and the coupling process is completed under the condition of not influencing the morphology, membrane structure and biological function of the extracellular vesicle; the tracing and drug loading research on the extracellular vesicles are facilitated..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 24. Nov. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
GE XIAOHU [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
Sonstige Themen: |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-11-24, Last update posted on www.tib.eu: 2024-02-05, Last updated: 2024-02-09 |
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| Patentnummer: |
CN117100873 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA019135645 |
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| 520 | |a The invention discloses a biological coupling method of an extracellular vesicle and an alpha, beta-unsaturated carbonyl compound, which comprises the following steps: catalyzing primary amine on the surface of the extracellular vesicle and the alpha, beta-unsaturated carbonyl compound to generate Michael addition reaction through proper alkali, and finishing direct coupling of the extracellular vesicle and the alpha, beta-unsaturated carbonyl compound through a one-step method; a compound containing an alpha, beta-unsaturated carbonyl structure characteristic, such as a fluorescent tracer or a medicinal natural product, can be directly coupled to the extracellular vesicle through the method disclosed by the invention, and the coupling process is completed under the condition of not influencing the morphology, membrane structure and biological function of the extracellular vesicle; the tracing and drug loading research on the extracellular vesicles are facilitated. | ||
| 650 | 4 | |a A61K: Preparations for medical, dental, or toilet purposes (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms a61j0003000000;chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles a61l; soap compositions c11d) | |
| 650 | 4 | |a A61P: Specific therapeutic activity of chemical compounds or medicinal preparations | |
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