Preparation method of pneumococcus capsular polysaccharide
The invention belongs to the technical field of biology, and particularly relates to a preparation method of pneumococcus capsular polysaccharide. The method comprises the following steps: (1) providing a pneumococcus fermentation broth, sterilizing and centrifuging the pneumococcus fermentation broth, collecting a first supernatant, and performing ultrafiltration to obtain a first ultrafiltrate; (2) adding neutral salt into the first ultrafiltrate, then mixing sodium deoxycholate to obtain a mixed solution, and adjusting the pH value to 4.5-5.0; centrifuging and collecting a second supernatant; the ratio of the sodium deoxycholate to the first ultrafiltrate is (0.4-0.7) g: 100mL; (3) adjusting the pH value of the second supernate back to be neutral, precipitating impurities through calcium salt, and centrifugally collecting third supernate; and drying the third supernate to obtain the pneumococcus capsular polysaccharide. The preparation method disclosed by the invention has the advantages of simple process, low cost, high pneumococcus capsular polysaccharide recovery rate, easiness in amplification, suitability for industrial application and the like..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 31. Okt. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
LIU YANLI [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-10-31, Last update posted on www.tib.eu: 2024-01-22, Last updated: 2024-01-26 |
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| Patentnummer: |
CN116970095 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA019040563 |
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| 520 | |a The invention belongs to the technical field of biology, and particularly relates to a preparation method of pneumococcus capsular polysaccharide. The method comprises the following steps: (1) providing a pneumococcus fermentation broth, sterilizing and centrifuging the pneumococcus fermentation broth, collecting a first supernatant, and performing ultrafiltration to obtain a first ultrafiltrate; (2) adding neutral salt into the first ultrafiltrate, then mixing sodium deoxycholate to obtain a mixed solution, and adjusting the pH value to 4.5-5.0; centrifuging and collecting a second supernatant; the ratio of the sodium deoxycholate to the first ultrafiltrate is (0.4-0.7) g: 100mL; (3) adjusting the pH value of the second supernate back to be neutral, precipitating impurities through calcium salt, and centrifugally collecting third supernate; and drying the third supernate to obtain the pneumococcus capsular polysaccharide. The preparation method disclosed by the invention has the advantages of simple process, low cost, high pneumococcus capsular polysaccharide recovery rate, easiness in amplification, suitability for industrial application and the like. | ||
| 650 | 4 | |a A61K: Preparations for medical, dental, or toilet purposes (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms a61j0003000000;chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles a61l; soap compositions c11d) | |
| 650 | 4 | |a A61P: Specific therapeutic activity of chemical compounds or medicinal preparations | |
| 650 | 4 | |a che | |
| 650 | 4 | |a C08B: Polysaccharides; derivatives thereof (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages c07h;fermentation or enzyme-using processes c12p0019000000;production of cellulose d21) | |
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