Preparation method and application of tortoise-shell glue polypeptide
The preparation method specifically comprises the following steps: (1) crushing tortoise plastron, adding water, carrying out heating reflux extraction, and filtering; (2) heating and continuously stirring and concentrating; (3) drying, namely drying again; (4) adding water for dissolving, then adding protease, and adjusting the temperature and the pH value for enzymolysis; and (5) heating, boiling, deactivating enzyme, cooling, centrifuging, and collecting supernate to obtain the traditional Chinese medicine composition. The tortoise-shell glue polypeptide (TSGH) is prepared by utilizing different enzymes, and potential anti-cancer peptides in the TSGH are predicted and explored by combining network pharmacology, a molecular docking technology and a calculation screening method. In addition, a hepatoma cell model is established, and the mechanism of the selected and synthesized peptides in treatment and prevention of hepatoma is researched. The invention finds the potential effects of FDF, FSG, LLLPKP and NGY on inhibiting proliferation of liver cancer cells, and clarifies the inhibiting effect of FDF on proliferation of liver cancer cells..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 26. Sept. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
ZHAO TIANTIAN [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-09-26, Last update posted on www.tib.eu: 2023-12-19, Last updated: 2023-12-22 |
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| Patentnummer: |
CN116804215 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA018826105 |
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| 520 | |a The preparation method specifically comprises the following steps: (1) crushing tortoise plastron, adding water, carrying out heating reflux extraction, and filtering; (2) heating and continuously stirring and concentrating; (3) drying, namely drying again; (4) adding water for dissolving, then adding protease, and adjusting the temperature and the pH value for enzymolysis; and (5) heating, boiling, deactivating enzyme, cooling, centrifuging, and collecting supernate to obtain the traditional Chinese medicine composition. The tortoise-shell glue polypeptide (TSGH) is prepared by utilizing different enzymes, and potential anti-cancer peptides in the TSGH are predicted and explored by combining network pharmacology, a molecular docking technology and a calculation screening method. In addition, a hepatoma cell model is established, and the mechanism of the selected and synthesized peptides in treatment and prevention of hepatoma is researched. The invention finds the potential effects of FDF, FSG, LLLPKP and NGY on inhibiting proliferation of liver cancer cells, and clarifies the inhibiting effect of FDF on proliferation of liver cancer cells. | ||
| 650 | 4 | |a bio | |
| 650 | 4 | |a C12P: Fermentation or enzyme-using processes to synthesise a desired chemical compound or composition or to separate optical isomers from a racemic mixture | |
| 650 | 4 | |a A61K: Preparations for medical, dental, or toilet purposes (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms a61j0003000000;chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles a61l; soap compositions c11d) | |
| 650 | 4 | |a A61P: Specific therapeutic activity of chemical compounds or medicinal preparations | |
| 650 | 4 | |a C07K: Peptides (peptides containing β-lactam rings c07d;cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, c07d;ergot alkaloids of the cyclic peptide type c07d0519020000; single cell proteins, enzymes c12n;genetic engineering processes for obtaining peptides c12n0015000000) | |
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