Method for culturing cells, and kit and apparatus therefor
Provided herein, in certain aspects, are methods relating to incubating or culturing a cell composition (e.g., a population of lymphocytes) (e.g., for induced stimulation of expansion (proliferation), activation, co-stimulation, and/or survival). In certain aspects, methods and reactants are provided for stimulating a population of cells, such as expansion (proliferation), survival or retention, activation, co-stimulation, or other effects, including binding an agent to a molecule on the surface of the cell to provide one or more signals to the cell. In some cases, the reactant is a reactant comprising a plurality of binding sites for an agent (e.g., a multimerization reactant) whereby one or more agents are multimerized by reversible binding to the reactant, e.g., thereby producing a stimulatory reactant (multimerized agent) having a stimulatory agent multimerized thereon. In some aspects, multimeric agents can be used for expansion or proliferation or other stimulation of cell populations, and then such stimulators can be removed by disruption of reversible bonds. Methods, compositions, and apparatuses for their use are also provided..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 24. Nov. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
GERMEROTH LOTHAR [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-11-24, Last update posted on www.tib.eu: 2024-03-04, Last updated: 2024-03-08 |
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| Patentnummer: |
CN117106716 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA018676693 |
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| 245 | 1 | 0 | |a Method for culturing cells, and kit and apparatus therefor |
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| 520 | |a Provided herein, in certain aspects, are methods relating to incubating or culturing a cell composition (e.g., a population of lymphocytes) (e.g., for induced stimulation of expansion (proliferation), activation, co-stimulation, and/or survival). In certain aspects, methods and reactants are provided for stimulating a population of cells, such as expansion (proliferation), survival or retention, activation, co-stimulation, or other effects, including binding an agent to a molecule on the surface of the cell to provide one or more signals to the cell. In some cases, the reactant is a reactant comprising a plurality of binding sites for an agent (e.g., a multimerization reactant) whereby one or more agents are multimerized by reversible binding to the reactant, e.g., thereby producing a stimulatory reactant (multimerized agent) having a stimulatory agent multimerized thereon. In some aspects, multimeric agents can be used for expansion or proliferation or other stimulation of cell populations, and then such stimulators can be removed by disruption of reversible bonds. Methods, compositions, and apparatuses for their use are also provided. | ||
| 650 | 4 | |a bio | |
| 650 | 4 | |a A61K: Preparations for medical, dental, or toilet purposes (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms a61j0003000000;chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles a61l; soap compositions c11d) | |
| 650 | 4 | |a C12N: Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media (microbiological testing media c12q0001000000) | |
| 650 | 4 | |a A61P: Specific therapeutic activity of chemical compounds or medicinal preparations | |
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