Broad-spectrum streptococcus genetic engineering lyase and medical application thereof
The invention is applicable to the technical field of bioengineering, and provides broad-spectrum streptococcus gene engineering lyase and medical application thereof.The nucleotide sequence of the broad-spectrum streptococcus gene engineering lyase is shown as SEQ ID NO.1 in a sequence table, and the amino acid sequence of the broad-spectrum streptococcus gene engineering lyase is shown as SEQ ID NO.2 in the sequence table. The construction method comprises the following steps: designing an upstream primer and a downstream primer according to the nucleotide sequence; amplifying a nucleotide sequence of the broad-spectrum streptococcus gene engineering lyase by using the upstream primer and the downstream primer, and cloning the nucleotide sequence into an expression vector to obtain a recombinant plasmid; transferring the recombinant plasmid into a strain to obtain a recombinant strain; and the recombinant strain is utilized to induce expression of broad-spectrum streptococcus gene engineering lyase. The broad-spectrum streptococcus gene engineering lyase provided by the embodiment of the invention shows relatively strong acid-base and high-temperature resistance in vitro, and can be used for remarkably removing a mature biological membrane formed by streptococcus suis and streptococcus equi..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 01. Sept. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
GU JINGMIN [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-09-01, Last update posted on www.tib.eu: 2023-11-21, Last updated: 2023-11-24 |
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| Patentnummer: |
CN116676299 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA018582397 |
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| 500 | |a Source: www.epo.org (no modifications made), First posted: 2023-09-01, Last update posted on www.tib.eu: 2023-11-21, Last updated: 2023-11-24 | ||
| 520 | |a The invention is applicable to the technical field of bioengineering, and provides broad-spectrum streptococcus gene engineering lyase and medical application thereof.The nucleotide sequence of the broad-spectrum streptococcus gene engineering lyase is shown as SEQ ID NO.1 in a sequence table, and the amino acid sequence of the broad-spectrum streptococcus gene engineering lyase is shown as SEQ ID NO.2 in the sequence table. The construction method comprises the following steps: designing an upstream primer and a downstream primer according to the nucleotide sequence; amplifying a nucleotide sequence of the broad-spectrum streptococcus gene engineering lyase by using the upstream primer and the downstream primer, and cloning the nucleotide sequence into an expression vector to obtain a recombinant plasmid; transferring the recombinant plasmid into a strain to obtain a recombinant strain; and the recombinant strain is utilized to induce expression of broad-spectrum streptococcus gene engineering lyase. The broad-spectrum streptococcus gene engineering lyase provided by the embodiment of the invention shows relatively strong acid-base and high-temperature resistance in vitro, and can be used for remarkably removing a mature biological membrane formed by streptococcus suis and streptococcus equi. | ||
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| 650 | 4 | |a A61K: Preparations for medical, dental, or toilet purposes (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms a61j0003000000;chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles a61l; soap compositions c11d) | |
| 650 | 4 | |a C12N: Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media (microbiological testing media c12q0001000000) | |
| 650 | 4 | |a A61P: Specific therapeutic activity of chemical compounds or medicinal preparations | |
| 650 | 4 | |a A01N: Preservation of bodies of humans or animals or plants or parts thereof (preservation of food or foodstuff a23); biocides, e.g. as disinfectants, as pesticides or as herbicides (preparations for medical, dental or toilet purposes which kill or prevent the growth or proliferation of unwanted organisms a61k); pest repellants or attractants; plant growth regulators | |
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