Nano nucleic acid probe as well as preparation method and application thereof
The invention provides a nano nucleic acid probe capable of effectively and quickly screening multiple tumor markers in real time. The nano nucleic acid probe consists of a DNA tetrahedron, a detection single-stranded DNA and an auxiliary single-stranded DNA, the DNA tetrahedron is formed by four single-stranded DNAs through base complementary pairing, the detection single-stranded DNA is provided with a fluorophore and a fluorescence quenching group, and the detection single-stranded DNA and the auxiliary single-stranded DNA are respectively connected with two different single strands of the DNA tetrahedron through base complementary pairing. The nano nucleic acid probe disclosed by the invention is high in detection sensitivity and good in biological safety, can enter cells by self-transport, does not need other carriers, is suitable for detecting intracellular targets of living cells, and can be used for animal living imaging to realize real-time fluorescence labeling of tumors..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 25. Juli Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
LIN YUNFENG [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-07-25, Last update posted on www.tib.eu: 2023-12-05, Last updated: 2023-12-08 |
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| Patentnummer: |
CN116479089 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA018267696 |
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| 520 | |a The invention provides a nano nucleic acid probe capable of effectively and quickly screening multiple tumor markers in real time. The nano nucleic acid probe consists of a DNA tetrahedron, a detection single-stranded DNA and an auxiliary single-stranded DNA, the DNA tetrahedron is formed by four single-stranded DNAs through base complementary pairing, the detection single-stranded DNA is provided with a fluorophore and a fluorescence quenching group, and the detection single-stranded DNA and the auxiliary single-stranded DNA are respectively connected with two different single strands of the DNA tetrahedron through base complementary pairing. The nano nucleic acid probe disclosed by the invention is high in detection sensitivity and good in biological safety, can enter cells by self-transport, does not need other carriers, is suitable for detecting intracellular targets of living cells, and can be used for animal living imaging to realize real-time fluorescence labeling of tumors. | ||
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| 650 | 4 | |a C09K: Materials for applications not otherwise provided for; applications of materials not otherwise provided for | |
| 650 | 4 | |a A61K: Preparations for medical, dental, or toilet purposes (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms a61j0003000000;chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles a61l; soap compositions c11d) | |
| 650 | 4 | |a G01N: Investigating or analysing materials by determining their chemical or physical properties (measuring or testing processes other than immunoassay, involving enzymes or microorganisms c12m, c12q) | |
| 650 | 4 | |a che | |
| 650 | 4 | |a C12Q: Measuring or testing processes involving enzymes, nucleic acids or microorganisms (immunoassay g01n0033530000); compositions or test papers therefor; processes of preparing such compositions; condition-responsive control in microbiological or enzymological processes | |
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