Application of peripheral CLU as target spot in reduction of brain A beta
The invention discloses application of peripheral CLU as a target spot in reduction of brain A beta. Specifically, the invention discloses application of peripheral CLU as a target spot in preparation of a product for preventing, improving or treating A beta related diseases. An RNAi-mediated gene knock-down method is taken as an example, and the effect of down-regulation of peripheral CLU on reduction of A beta load in the brain is elaborated. According to a mouse CLU gene sequence and an RNAi principle, by utilizing a short hairpin RNA sequence, the recombinant adeno-associated virus is cloned into an AAV8 adeno-associated virus expression vector, and the recombinant adeno-associated virus carrying knock-down shRNA (shRNA1) is obtained. Through intraperitoneal injection of recombinant adeno-associated virus into a transgenic mouse expressing human CLU (hCLU), it is verified that A beta in the brain can be remarkably reduced (the amount and deposition of beta-amyloid protein in the brain are inhibited) by reducing the expression or activity of the CLU gene in peripheral blood, and the excretion function of the CLU on the A beta in the brain can be played to the greatest extent..
Medienart: |
Patent |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Europäisches Patentamt - (2023) vom: 14. Apr. Zur Gesamtaufnahme - year:2023 |
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Sprache: |
Englisch |
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Beteiligte Personen: |
WANG ZHE [VerfasserIn] |
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Links: |
Volltext [kostenfrei] |
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Themen: |
Sonstige Themen: |
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Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-04-14, Last update posted on www.tib.eu: 2023-09-19, Last updated: 2023-09-22 |
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Patentnummer: |
CN115957324 |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
EPA018001211 |
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520 | |a The invention discloses application of peripheral CLU as a target spot in reduction of brain A beta. Specifically, the invention discloses application of peripheral CLU as a target spot in preparation of a product for preventing, improving or treating A beta related diseases. An RNAi-mediated gene knock-down method is taken as an example, and the effect of down-regulation of peripheral CLU on reduction of A beta load in the brain is elaborated. According to a mouse CLU gene sequence and an RNAi principle, by utilizing a short hairpin RNA sequence, the recombinant adeno-associated virus is cloned into an AAV8 adeno-associated virus expression vector, and the recombinant adeno-associated virus carrying knock-down shRNA (shRNA1) is obtained. Through intraperitoneal injection of recombinant adeno-associated virus into a transgenic mouse expressing human CLU (hCLU), it is verified that A beta in the brain can be remarkably reduced (the amount and deposition of beta-amyloid protein in the brain are inhibited) by reducing the expression or activity of the CLU gene in peripheral blood, and the excretion function of the CLU on the A beta in the brain can be played to the greatest extent. | ||
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