Preparation method of rabies vaccine strain
The invention discloses a preparation method of a rabies vaccine strain. The preparation method comprises the following steps that rabies virus is cultured and liquid is collected; BHK-21 cells are taken, a growth solution is added, culturing is carried out in a carbon dioxide incubator at 37 DEG C, after the cells grow into a single layer, 20 bottles (20cm<2> cell bottles) of cells are divided into two groups according to a random mode, rRC-HL and rRC-HLdeltaN virus seeds are correspondingly added overnight, a maintenance solution is changed, the two groups are inoculated for 1 week, the liquid is collected, and rabies vaccines of two different strains are produced and compared. According to the preparation method of the rabies vaccine strain, 103 and 106FFU are correspondingly adopted to perform intracerebral inoculation on adult Kunming mice, the adult Kunming mice cannot be killed, and the level of antibodies generated after immunization is high..
Medienart: |
Patent |
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Erscheinungsjahr: |
2022 |
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Erschienen: |
2022 |
Enthalten in: |
Europäisches Patentamt - (2022) vom: 04. Jan. Zur Gesamtaufnahme - year:2022 |
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Sprache: |
Englisch |
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Beteiligte Personen: |
WEI XIANKAI [VerfasserIn] |
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Links: |
Volltext [kostenfrei] |
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Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2022-01-04, Last update posted on www.tib.eu: 2023-08-07, Last updated: 2023-08-11 |
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Patentnummer: |
CN113881639 |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
EPA013234781 |
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245 | 1 | 0 | |a Preparation method of rabies vaccine strain |
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520 | |a The invention discloses a preparation method of a rabies vaccine strain. The preparation method comprises the following steps that rabies virus is cultured and liquid is collected; BHK-21 cells are taken, a growth solution is added, culturing is carried out in a carbon dioxide incubator at 37 DEG C, after the cells grow into a single layer, 20 bottles (20cm<2> cell bottles) of cells are divided into two groups according to a random mode, rRC-HL and rRC-HLdeltaN virus seeds are correspondingly added overnight, a maintenance solution is changed, the two groups are inoculated for 1 week, the liquid is collected, and rabies vaccines of two different strains are produced and compared. According to the preparation method of the rabies vaccine strain, 103 and 106FFU are correspondingly adopted to perform intracerebral inoculation on adult Kunming mice, the adult Kunming mice cannot be killed, and the level of antibodies generated after immunization is high. | ||
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