Dendritic cell modification method based on cell membrane surface modification technology and application thereof
The invention discloses a dendritic cell modification method based on a cell membrane surface modification technology and application of the dendritic cell modification method. The dendritic cell modification method based on the cell membrane surface modification technology, provided by the invention, comprises the following steps: 1) synthesizing a base material for photo-thermal transfection; 2) coating a plasmid containing a gene sequence for coding HTP with PEI, and placing the plasmid on the surface of the base material; (3) preparing modified dendritic cells; 4) synthesizing a functional glycopolymer molecule; 5) mixing the functional glycopolymer molecules and the modified dendritic cells, and performing incubation. According to the dendritic cell modification method based on the cell membrane surface modification technology, the surface of a DC cell can be efficiently modified with a glycopolymer with a specific recognition effect, so the interaction between the DC cell and a T cell is improved, and finally, the activation efficiency of the T cell is improved; meanwhile, the cell transfection efficiency, the activity of transfected cells and the overall safety of a transfection system are improved..
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Patent| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Europäisches Patentamt - (2021) vom: 24. Aug. Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
CHEN HONG [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2021-08-24, Last update posted on www.tib.eu: 2022-04-12, Last updated: 2023-02-09 |
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| Patentnummer: |
CN113293137 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA012218987 |
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| 520 | |a The invention discloses a dendritic cell modification method based on a cell membrane surface modification technology and application of the dendritic cell modification method. The dendritic cell modification method based on the cell membrane surface modification technology, provided by the invention, comprises the following steps: 1) synthesizing a base material for photo-thermal transfection; 2) coating a plasmid containing a gene sequence for coding HTP with PEI, and placing the plasmid on the surface of the base material; (3) preparing modified dendritic cells; 4) synthesizing a functional glycopolymer molecule; 5) mixing the functional glycopolymer molecules and the modified dendritic cells, and performing incubation. According to the dendritic cell modification method based on the cell membrane surface modification technology, the surface of a DC cell can be efficiently modified with a glycopolymer with a specific recognition effect, so the interaction between the DC cell and a T cell is improved, and finally, the activation efficiency of the T cell is improved; meanwhile, the cell transfection efficiency, the activity of transfected cells and the overall safety of a transfection system are improved. | ||
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