Method for measuring temperature change of organelle
The invention relates to a method for measuring the temperature change of an organelle. The method comprises the steps of preparing a temperature-sensitive water-soluble CdTe/CdS/ZnS quantum dot solution, and coating silicon dioxide on the temperature-sensitive water-soluble CdTe/CdS/ZnS quantum dot solution by using a reverse microemulsion method to obtain a CdTe/CdS/ZnS@SiO2 particle solution; then preparing a positioning probe CdTe/CdS/ZnS@SiO2-Cyto, a lysosome targeting positioning probe CdTe/CdS/ZnS@ SiO2-Lyso or a mitochondria targeting positioning probe CdTe/CdS/ZnS@SiO2-Mito for ingesting cytoplasm in cells; respectively taking positioning probes into cells through a liposome transfection method, carrying out targeted positioning in intracellular cytoplasm or on different organelles through different targeted modifications, combining with the temperature-sensitive characteristics of CdTe/CdS/ZnS quantum dots, and realizing acquisition of temperature-sensitive fluorescence information of quantum dots of a targeted organelle through the action of combining an inverted fluorescence microscope with slit locking of a spectrograph, so that temperature change information in cytoplasm or temperature change information of the targeted localized organelle is obtained. According to the invention, targeting cytoplasm and specific organelles (such as lysosome and mitochondria) and monitoring the temperature change of the cytoplasm and specific organelles are realized..
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Patent| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Europäisches Patentamt - (2021) vom: 20. Apr. Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
JIANG XINBING [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2021-04-20, Last update posted on www.tib.eu: 2022-04-11, Last updated: 2023-02-09 |
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| Patentnummer: |
CN112683417 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA012141194 |
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| 520 | |a The invention relates to a method for measuring the temperature change of an organelle. The method comprises the steps of preparing a temperature-sensitive water-soluble CdTe/CdS/ZnS quantum dot solution, and coating silicon dioxide on the temperature-sensitive water-soluble CdTe/CdS/ZnS quantum dot solution by using a reverse microemulsion method to obtain a CdTe/CdS/ZnS@SiO2 particle solution; then preparing a positioning probe CdTe/CdS/ZnS@SiO2-Cyto, a lysosome targeting positioning probe CdTe/CdS/ZnS@ SiO2-Lyso or a mitochondria targeting positioning probe CdTe/CdS/ZnS@SiO2-Mito for ingesting cytoplasm in cells; respectively taking positioning probes into cells through a liposome transfection method, carrying out targeted positioning in intracellular cytoplasm or on different organelles through different targeted modifications, combining with the temperature-sensitive characteristics of CdTe/CdS/ZnS quantum dots, and realizing acquisition of temperature-sensitive fluorescence information of quantum dots of a targeted organelle through the action of combining an inverted fluorescence microscope with slit locking of a spectrograph, so that temperature change information in cytoplasm or temperature change information of the targeted localized organelle is obtained. According to the invention, targeting cytoplasm and specific organelles (such as lysosome and mitochondria) and monitoring the temperature change of the cytoplasm and specific organelles are realized. | ||
| 650 | 4 | |a cet | |
| 650 | 4 | |a G01K: Measuring temperature; measuring quantity of heat; thermally-sensitive elements not otherwise provided for (radiation pyrometry g01j0005000000) | |
| 650 | 4 | |a B82Y: Specific uses or applications of nanostructures; measurement or analysis of nanostructures; manufacture or treatment of nanostructures | |
| 650 | 4 | |a C09K: Materials for applications not otherwise provided for; applications of materials not otherwise provided for | |
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