METHOD OF OBTAINING MICROENCAPSULATED FORMS OF LIVE CULTURE VACCINE AGAINST SEASONAL AND PANDEMIC INFLUENZA FOR INTRANASAL USE
FIELD: biotechnology.SUBSTANCE: method includes obtaining a substance of influenza virus, a simultaneous introduction of a hardener and charged water-soluble polyeloctrolyte in the virus-containing substance to reach the final concentration of the polyelectrolyte and hardener which does not cause a phase separation in the medication solution. Further, the microcapsules are produced resulting from a composite coacervation of the charged polyelectrolyte and the hardener with a formation of a complex by freezing the solution at a speed of 0.1-3.0°C per minute to the temperature which is lower than the glass transition temperature of amorphous phase remaining after the ice crystallization and freeze-drying of the final microcapsules. The substance of the influenza virus is produced with a specific activity of not less than 7.0 lgEID/0.5 ml in a serum-free medium containing a proteolytic enzyme in the amount of 0.25-50.0 mcg/ml and soy peptone stabiliser in a concentration of (0.5-4.0) wt %. Stabilizing soy peptone additives and sucrose are added to the purified substance together with the polyelectrolyte and hardener. Carbopol is used as a polyelectrolyte and gelatose is used as a hardener at the following quantitative component content in the resulting liquid substance prior to microencapsulation: soy peptone - (0.007-0.015) g/0.5 ml; sucrose - (0.007-0.015) g/0.5 ml; gelatose - (0.007-0.015) g/0.5 ml; carbopol - (0.00005-0.0001) g/0.5 ml; liquid nutritional medium containing influenza virus with a titre of not less than 10EID. The rest is up to 0.5 ml.EFFECT: use of this method for producing live-culture influenza vaccine in microcapsules provides for a higher immunogenicity in intranasal application due to increasing the adhesion of microcapsules to nasal mucosa.3 cl, 6 ex, 1 tbl, 1 dwg.
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Patent| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Europäisches Patentamt - (2017) vom: 19. Apr. Zur Gesamtaufnahme - year:2017 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
NECHAEVA ELENA AVGUSTOVNA [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2017-04-19, Last update posted on www.tib.eu: 2022-05-31, Last updated: 2023-02-09 |
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| Patentnummer: |
RU2617051 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA008313504 |
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| 245 | 1 | 0 | |a METHOD OF OBTAINING MICROENCAPSULATED FORMS OF LIVE CULTURE VACCINE AGAINST SEASONAL AND PANDEMIC INFLUENZA FOR INTRANASAL USE |
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| 520 | |a FIELD: biotechnology.SUBSTANCE: method includes obtaining a substance of influenza virus, a simultaneous introduction of a hardener and charged water-soluble polyeloctrolyte in the virus-containing substance to reach the final concentration of the polyelectrolyte and hardener which does not cause a phase separation in the medication solution. Further, the microcapsules are produced resulting from a composite coacervation of the charged polyelectrolyte and the hardener with a formation of a complex by freezing the solution at a speed of 0.1-3.0°C per minute to the temperature which is lower than the glass transition temperature of amorphous phase remaining after the ice crystallization and freeze-drying of the final microcapsules. The substance of the influenza virus is produced with a specific activity of not less than 7.0 lgEID/0.5 ml in a serum-free medium containing a proteolytic enzyme in the amount of 0.25-50.0 mcg/ml and soy peptone stabiliser in a concentration of (0.5-4.0) wt %. Stabilizing soy peptone additives and sucrose are added to the purified substance together with the polyelectrolyte and hardener. Carbopol is used as a polyelectrolyte and gelatose is used as a hardener at the following quantitative component content in the resulting liquid substance prior to microencapsulation: soy peptone - (0.007-0.015) g/0.5 ml; sucrose - (0.007-0.015) g/0.5 ml; gelatose - (0.007-0.015) g/0.5 ml; carbopol - (0.00005-0.0001) g/0.5 ml; liquid nutritional medium containing influenza virus with a titre of not less than 10EID. The rest is up to 0.5 ml.EFFECT: use of this method for producing live-culture influenza vaccine in microcapsules provides for a higher immunogenicity in intranasal application due to increasing the adhesion of microcapsules to nasal mucosa.3 cl, 6 ex, 1 tbl, 1 dwg | ||
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