Trolliol A as well as preparation method and application thereof
The invention discloses trolliol A as well as a preparation method and application thereof, and relates to a novel substance with in-vitro anti-inflammation and a preparation method and application thereof. The invention provides a novel substance with in-vitro anti-inflammatory activity and a preparation method and application thereof. The chemical name of the trolliol A is 1-(3, 4-dihydroxyphenyl)-3-hydroxyphenylpan-2-one, and the molecular formula of the trolliol A is C9H10O4. The invention further discloses a preparation method of the trolliol A. The preparation method comprises the following steps: 1, extracting trollius chinensis bunge with ethanol with the volume concentration of 70%; 2, dispersing the extract with recovered ethanol with water, and loading on a D101 macroporous resin column; and 3, carrying out repeated silica gel column chromatography separation on the D101 macroporous resin 30% ethanol elution fraction, and preparing by using high performance liquid chromatography to obtain a monomeric compound which is identified as a new compound through spectroscopy and named as trolliol A. The trolliol A is applied to preparation of anti-inflammatory drugs. The IC50 of the inhibition rates of the trolliol A on proinflammatory factors TNF-alpha and IL-1beta released by LPS (lipopolysaccharide) stimulated mouse macrophages (RAW264.7) is 18.8 mu M and 14.6 mu M respectively, so that the trolliol A has better in-vitro anti-inflammatory activity. The invention belongs to the field of research on effective components of natural medicines..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 29. Sept. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
LIU JICHENG [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-09-29, Last update posted on www.tib.eu: 2024-04-22, Last updated: 2024-04-26 |
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| Patentnummer: |
CN116813460 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA003789985 |
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| 520 | |a The invention discloses trolliol A as well as a preparation method and application thereof, and relates to a novel substance with in-vitro anti-inflammation and a preparation method and application thereof. The invention provides a novel substance with in-vitro anti-inflammatory activity and a preparation method and application thereof. The chemical name of the trolliol A is 1-(3, 4-dihydroxyphenyl)-3-hydroxyphenylpan-2-one, and the molecular formula of the trolliol A is C9H10O4. The invention further discloses a preparation method of the trolliol A. The preparation method comprises the following steps: 1, extracting trollius chinensis bunge with ethanol with the volume concentration of 70%; 2, dispersing the extract with recovered ethanol with water, and loading on a D101 macroporous resin column; and 3, carrying out repeated silica gel column chromatography separation on the D101 macroporous resin 30% ethanol elution fraction, and preparing by using high performance liquid chromatography to obtain a monomeric compound which is identified as a new compound through spectroscopy and named as trolliol A. The trolliol A is applied to preparation of anti-inflammatory drugs. The IC50 of the inhibition rates of the trolliol A on proinflammatory factors TNF-alpha and IL-1beta released by LPS (lipopolysaccharide) stimulated mouse macrophages (RAW264.7) is 18.8 mu M and 14.6 mu M respectively, so that the trolliol A has better in-vitro anti-inflammatory activity. The invention belongs to the field of research on effective components of natural medicines. | ||
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| 650 | 4 | |a A61P: Specific therapeutic activity of chemical compounds or medicinal preparations | |
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