广谱人源化抗新型冠状病毒单克隆抗体及应用
本发明公开了一种广谱人源化抗SARS‑COV‑2病毒的单克隆中和抗体,本发明的中和抗体通过单个B细胞流式分选‑抗体基因扩增配对表达技术筛选获得,具有独特的CDR区域,能特异性与SARS‑COV‑2结合并且可以有效中和目前世界卫生组织定义的“值得关切的变异株”(SARS‑CoV‑2 WT,alpha,Beta,Gamma,Delta,Omicron),尤其是中和Omicron变异毒株的IC50均低于0.1μg/mL。本发明还涉及该组中和抗体的制备方法和用途。本发明的4株抗体可以用于针对COVID‑19变异毒株全球流行的紧急预防和或治疗,具有全人源化,高表达,稳定性好的特点,适合产业化。此外,该抗体还可用于制备SARS‑COV‑2病毒检测试剂,发现有效中和抗原表位及开发SARS‑COV‑2重组蛋白及亚单位疫苗。.
Medienart: |
Patent |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Europäisches Patentamt - (2024) vom: 02. Apr. Zur Gesamtaufnahme - year:2024 |
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Sprache: |
Chinesisch |
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Links: |
Volltext [kostenfrei] |
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Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2024-04-02, Last update posted on www.tib.eu: 2024-04-17, Last updated: 2024-04-26 |
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Patentnummer: |
CN114409774 |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
EPA003474011 |
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520 | |a 本发明公开了一种广谱人源化抗SARS‑COV‑2病毒的单克隆中和抗体,本发明的中和抗体通过单个B细胞流式分选‑抗体基因扩增配对表达技术筛选获得,具有独特的CDR区域,能特异性与SARS‑COV‑2结合并且可以有效中和目前世界卫生组织定义的“值得关切的变异株”(SARS‑CoV‑2 WT,alpha,Beta,Gamma,Delta,Omicron),尤其是中和Omicron变异毒株的IC50均低于0.1μg/mL。本发明还涉及该组中和抗体的制备方法和用途。本发明的4株抗体可以用于针对COVID‑19变异毒株全球流行的紧急预防和或治疗,具有全人源化,高表达,稳定性好的特点,适合产业化。此外,该抗体还可用于制备SARS‑COV‑2病毒检测试剂,发现有效中和抗原表位及开发SARS‑COV‑2重组蛋白及亚单位疫苗。 | ||
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