Method for stably knocking out CBX4 gene in osteosarcoma cell 143B
The invention provides a method for stably knocking out a CBX4 gene in an osteosarcoma cell 143B, belongs to the technical field of biology, and particularly relates to the method for stably knocking out the CBX4 gene in the osteosarcoma cell 143B. The invention relates to a method for stably knocking out a CBX4 gene in an osteosarcoma cell 143B, which comprises the following steps: editing the CBX4 gene in an osteosarcoma cell 143B genome by using a CRISPR/Cas9 system, and knocking out the CBX4 gene to obtain a human osteosarcoma cell 143B of which the CBX4 gene is stably knocked out. The method disclosed by the invention is short in construction time and simple to operate, and proliferation and metastasis of the obtained osteosarcoma cell 143B of which the CBX4 is stably knocked out are remarkably inhibited..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 25. Apr. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
ZHANG QI [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-04-25, Last update posted on www.tib.eu: 2023-09-26, Last updated: 2023-09-29 |
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| Patentnummer: |
CN116004723 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA002037599 |
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| 245 | 1 | 0 | |a Method for stably knocking out CBX4 gene in osteosarcoma cell 143B |
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| 520 | |a The invention provides a method for stably knocking out a CBX4 gene in an osteosarcoma cell 143B, belongs to the technical field of biology, and particularly relates to the method for stably knocking out the CBX4 gene in the osteosarcoma cell 143B. The invention relates to a method for stably knocking out a CBX4 gene in an osteosarcoma cell 143B, which comprises the following steps: editing the CBX4 gene in an osteosarcoma cell 143B genome by using a CRISPR/Cas9 system, and knocking out the CBX4 gene to obtain a human osteosarcoma cell 143B of which the CBX4 gene is stably knocked out. The method disclosed by the invention is short in construction time and simple to operate, and proliferation and metastasis of the obtained osteosarcoma cell 143B of which the CBX4 is stably knocked out are remarkably inhibited. | ||
| 650 | 4 | |a C12N: Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media (microbiological testing media c12q0001000000) | |
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