Eudermal papilla cell spheres, in-vitro culture method thereof, vesicles and application
The invention belongs to the field of biological materials and regenerative medicine, and particularly relates to a dermal papilla cell sphere, an in-vitro culture method thereof, a vesicle and application. The dermal papilla cell sphere is prepared according to the following method: extracting primary dermal papilla cells from in-vitro dermal papilla tissues, and subculturing for 3-5 generations; the method comprises the following steps: inoculating the passage of the dermal papilla cells into an SLF-3D culture system, culturing, collecting semi-suspended spheres when the growth fusion degree of the dermal papilla cells is 90-100%, separating the spheres into single cells, inoculating the single cells into the original SLF-3D culture system again, culturing for 3-5 days, and collecting the formed suspended spheres, namely the dermal papilla cell spheres. The dermal papilla cell ball and the vesicle thereof provided by the invention have a better treatment effect of scar-free wound healing..
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Patent| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Europäisches Patentamt - (2023) vom: 25. Apr. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
WANG YUNWEI [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Anmerkungen: |
Source: www.epo.org (no modifications made), First posted: 2023-04-25, Last update posted on www.tib.eu: 2023-09-26, Last updated: 2023-09-29 |
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| Patentnummer: |
CN116004529 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
EPA002037238 |
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| 520 | |a The invention belongs to the field of biological materials and regenerative medicine, and particularly relates to a dermal papilla cell sphere, an in-vitro culture method thereof, a vesicle and application. The dermal papilla cell sphere is prepared according to the following method: extracting primary dermal papilla cells from in-vitro dermal papilla tissues, and subculturing for 3-5 generations; the method comprises the following steps: inoculating the passage of the dermal papilla cells into an SLF-3D culture system, culturing, collecting semi-suspended spheres when the growth fusion degree of the dermal papilla cells is 90-100%, separating the spheres into single cells, inoculating the single cells into the original SLF-3D culture system again, culturing for 3-5 days, and collecting the formed suspended spheres, namely the dermal papilla cell spheres. The dermal papilla cell ball and the vesicle thereof provided by the invention have a better treatment effect of scar-free wound healing. | ||
| 650 | 4 | |a C12N: Microorganisms or enzymes; compositions thereof; propagating, preserving, or maintaining microorganisms; mutation or genetic engineering; culture media (microbiological testing media c12q0001000000) | |
| 650 | 4 | |a A61Q: Specific use of cosmetics or similar toilet preparations | |
| 650 | 4 | |a A61P: Specific therapeutic activity of chemical compounds or medicinal preparations | |
| 650 | 4 | |a che | |
| 650 | 4 | |a A61K: Preparations for medical, dental, or toilet purposes (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms a61j0003000000;chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles a61l; soap compositions c11d) | |
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