Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics
The Spatholobus littoralis Hassk. (S. littoralis Hassk.) is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non-communicable degenerative diseases. One of these illnesses, known as gout, is caused by excessive uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated the inhibition kinetics of XO and identified bioactive compounds from the stem extract fractions of S. littoralis Hassk. using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Fractionation was carried out to obtain n-hexane, dichloromethane, and 1-butanol fractions from the water and 70% ethanol extracts. Fraction of 1-butanol from 70% ethanol and water extract displayed potent inhibitors of XO with IC50 value 116.91±3.51 and 137.15±5.00 mg/L, respectively. Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The 1-butanol fraction from the two extracts has been further identified as a bioactive fraction. The majority of the compounds in the two active fractions were phenolics. These findings revealed that the 1-butanol fraction from the two extracts is promising as an antigout treatment in the future..
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2023 |
---|---|
Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
---|---|
Enthalten in: |
Hayati Journal of Biosciences - 31(2023), 1 |
Sprache: |
Englisch ; Indonesisch |
---|
Beteiligte Personen: |
Rut Novalia Rahmawati Sianipar [VerfasserIn] |
---|
Links: |
doi.org [kostenfrei] |
---|
Themen: |
---|
doi: |
10.4308/hjb.31.1.1-20 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
DOAJ098375849 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | DOAJ098375849 | ||
003 | DE-627 | ||
005 | 20240413222034.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240413s2023 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.4308/hjb.31.1.1-20 |2 doi | |
035 | |a (DE-627)DOAJ098375849 | ||
035 | |a (DE-599)DOAJ518a59a34a69408980f716e3c6fa9854 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng |a ind | ||
050 | 0 | |a QH301-705.5 | |
100 | 0 | |a Rut Novalia Rahmawati Sianipar |e verfasserin |4 aut | |
245 | 1 | 0 | |a Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics |
264 | 1 | |c 2023 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a The Spatholobus littoralis Hassk. (S. littoralis Hassk.) is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non-communicable degenerative diseases. One of these illnesses, known as gout, is caused by excessive uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated the inhibition kinetics of XO and identified bioactive compounds from the stem extract fractions of S. littoralis Hassk. using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Fractionation was carried out to obtain n-hexane, dichloromethane, and 1-butanol fractions from the water and 70% ethanol extracts. Fraction of 1-butanol from 70% ethanol and water extract displayed potent inhibitors of XO with IC50 value 116.91±3.51 and 137.15±5.00 mg/L, respectively. Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The 1-butanol fraction from the two extracts has been further identified as a bioactive fraction. The majority of the compounds in the two active fractions were phenolics. These findings revealed that the 1-butanol fraction from the two extracts is promising as an antigout treatment in the future. | ||
653 | 0 | |a Biology (General) | |
700 | 0 | |a Komar Sutriah |e verfasserin |4 aut | |
700 | 0 | |a Dyah Iswantini |e verfasserin |4 aut | |
700 | 0 | |a Trivadila |e verfasserin |4 aut | |
700 | 0 | |a Suminar Setiati Achmadi |e verfasserin |4 aut | |
773 | 0 | 8 | |i In |t Hayati Journal of Biosciences |d Bogor Agricultural University, 2010 |g 31(2023), 1 |w (DE-627)DOAJ000145815 |x 20864094 |7 nnns |
773 | 1 | 8 | |g volume:31 |g year:2023 |g number:1 |
856 | 4 | 0 | |u https://doi.org/10.4308/hjb.31.1.1-20 |z kostenfrei |
856 | 4 | 0 | |u https://doaj.org/article/518a59a34a69408980f716e3c6fa9854 |z kostenfrei |
856 | 4 | 0 | |u https://journal.ipb.ac.id/index.php/hayati/article/view/46469 |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/1978-3019 |y Journal toc |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/2086-4094 |y Journal toc |z kostenfrei |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_DOAJ | ||
951 | |a AR | ||
952 | |d 31 |j 2023 |e 1 |