Analysis of Interleukin-6 level in Serum and Histopathology Changes of Cardiovascular Tissue on Male Wistar Rat with Nonalcoholic Fatty Liver Disease (NAFLD)
Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic hepatopathy and a global health issue. Interleukin-6 (IL-6) in liver pathology is very complex, and its participation in the development of NAFLD remains unclear. This study aimed to analyze the relationship between NAFLD and IL-6 as one of inflammaging biomarkers along with CVD histopathology. Methods: This study used a post-test control group design. The subject of this study was 14 male Wistar rats and divided into two groups. The control group was given standard diet and the NAFLD-modelling group was given 8 weeks high-fat-high-fructose diet. Both groups will be terminated at the 8th week for organ histopathological examination. Results: The IL-6 levels in NAFLD rats (16.14±6.46ng/ml) and non-NAFLD rats (11.28±3.49ng/ml). The blood vessel wall thickness of NAFLD (1.47±0.33 µm) and non-NAFLD rats (1.70±0.28 µm). The diameter of the blood vessels of NAFLD rats (14.06±3.30 m) and non-NAFLD rats (15.66±1.02 m). It was found that there were differences in cardiac histopathology (hemorrhagic (p:0.003), degenerated (p:0.036), and hyperemic(p:0.003)) between NAFLD and non-NAFLD modeling rats. As for IL-6 levels, blood vessel wall thickness, diameter, and heart histopathology (necrosis) there was no difference between NAFLD and non-NAFLD modeling rats (p<0.05). Conclusion: It has shown that NAFLD rats significantly caused cardiac histopathological changes. The levels of IL-6 tend to be higher in NAFLD rats but not significant, as well as the diameter of coronary artery shows a small tendency in NAFLD rats..
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
---|---|
Enthalten in: |
Indonesia Journal of Biomedical Science - 15(2021), 2, Seite 174-179 |
Sprache: |
Englisch ; Indonesisch |
---|
Beteiligte Personen: |
Mylda Pratiwi Ahmadwirawan [VerfasserIn] |
---|
Links: |
doi.org [kostenfrei] |
---|
Themen: |
Cardiovascular histopathology |
---|
doi: |
10.15562/ijbs.v15i2.321 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
DOAJ091940052 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | DOAJ091940052 | ||
003 | DE-627 | ||
005 | 20240412110555.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240412s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.15562/ijbs.v15i2.321 |2 doi | |
035 | |a (DE-627)DOAJ091940052 | ||
035 | |a (DE-599)DOAJ2df8058f7a704ff8883b9a5e4fe76887 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng |a ind | ||
050 | 0 | |a R5-920 | |
100 | 0 | |a Mylda Pratiwi Ahmadwirawan |e verfasserin |4 aut | |
245 | 1 | 0 | |a Analysis of Interleukin-6 level in Serum and Histopathology Changes of Cardiovascular Tissue on Male Wistar Rat with Nonalcoholic Fatty Liver Disease (NAFLD) |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic hepatopathy and a global health issue. Interleukin-6 (IL-6) in liver pathology is very complex, and its participation in the development of NAFLD remains unclear. This study aimed to analyze the relationship between NAFLD and IL-6 as one of inflammaging biomarkers along with CVD histopathology. Methods: This study used a post-test control group design. The subject of this study was 14 male Wistar rats and divided into two groups. The control group was given standard diet and the NAFLD-modelling group was given 8 weeks high-fat-high-fructose diet. Both groups will be terminated at the 8th week for organ histopathological examination. Results: The IL-6 levels in NAFLD rats (16.14±6.46ng/ml) and non-NAFLD rats (11.28±3.49ng/ml). The blood vessel wall thickness of NAFLD (1.47±0.33 µm) and non-NAFLD rats (1.70±0.28 µm). The diameter of the blood vessels of NAFLD rats (14.06±3.30 m) and non-NAFLD rats (15.66±1.02 m). It was found that there were differences in cardiac histopathology (hemorrhagic (p:0.003), degenerated (p:0.036), and hyperemic(p:0.003)) between NAFLD and non-NAFLD modeling rats. As for IL-6 levels, blood vessel wall thickness, diameter, and heart histopathology (necrosis) there was no difference between NAFLD and non-NAFLD modeling rats (p<0.05). Conclusion: It has shown that NAFLD rats significantly caused cardiac histopathological changes. The levels of IL-6 tend to be higher in NAFLD rats but not significant, as well as the diameter of coronary artery shows a small tendency in NAFLD rats. | ||
650 | 4 | |a interleukin-6 (il-6) | |
650 | 4 | |a cardiovascular histopathology | |
650 | 4 | |a non-alcoholic fatty liver disease (nafld) | |
650 | 4 | |a wistar | |
653 | 0 | |a Medicine (General) | |
700 | 0 | |a Husni Cangara |e verfasserin |4 aut | |
700 | 0 | |a Arif Santoso |e verfasserin |4 aut | |
700 | 0 | |a Marhaen Hardjo |e verfasserin |4 aut | |
700 | 0 | |a Endy Adnan |e verfasserin |4 aut | |
700 | 0 | |a Firdaus Hamid |e verfasserin |4 aut | |
773 | 0 | 8 | |i In |t Indonesia Journal of Biomedical Science |d Universitas Udayana, 2017 |g 15(2021), 2, Seite 174-179 |w (DE-627)DOAJ000107603 |x 23022906 |7 nnns |
773 | 1 | 8 | |g volume:15 |g year:2021 |g number:2 |g pages:174-179 |
856 | 4 | 0 | |u https://doi.org/10.15562/ijbs.v15i2.321 |z kostenfrei |
856 | 4 | 0 | |u https://doaj.org/article/2df8058f7a704ff8883b9a5e4fe76887 |z kostenfrei |
856 | 4 | 0 | |u https://ijbs-udayana.org/index.php/ijbs/article/view/321 |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/2302-2906 |y Journal toc |z kostenfrei |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_DOAJ | ||
951 | |a AR | ||
952 | |d 15 |j 2021 |e 2 |h 174-179 |