Selection and Validation of qRT-PCR Internal Reference Genes to Study Flower Color Formation in <i<Camellia impressinervis</i<
Real-time quantitative PCR (qRT-PCR) is a pivotal technique for gene expression analysis. To ensure reliable and accurate results, the internal reference genes must exhibit stable expression across varied experimental conditions. Currently, no internal reference genes for <i<Camellia impressinervis</i< have been established. This study aimed to identify stable internal reference genes from eight candidates derived from different developmental stages of <i<C. impressinervis</i< flowers. We employed geNorm, NormFinder, and BestKeeper to evaluate the expression stability of these candidates, which was followed by a comprehensive stability analysis. The results indicated that <i<CiTUB</i<, a tubulin gene, exhibited the most stable expression among the eight reference gene candidates in the petals. Subsequently, <i<CiTUB</i< was utilized as an internal reference for the qRT-PCR analysis of six genes implicated in the petal pigment synthesis pathway of <i<C. impressinervis</i<. The qRT-PCR results were corroborated by transcriptome sequencing data, affirming the stability and suitability of <i<CiTUB</i< as a reference gene. This study marks the first identification of stable internal reference genes within the entire genome of <i<C. impressinervis</i<, establishing a foundation for future gene expression and functional studies. Identifying such stable reference genes is crucial for advancing molecular research on <i<C. impressinervis</i<..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
International Journal of Molecular Sciences - 25(2024), 5, p 3029 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Peilan Zhang [VerfasserIn] |
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| Links: |
doi.org [kostenfrei] |
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| Themen: |
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| doi: |
10.3390/ijms25053029 |
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| PPN (Katalog-ID): |
DOAJ091255724 |
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| 520 | |a Real-time quantitative PCR (qRT-PCR) is a pivotal technique for gene expression analysis. To ensure reliable and accurate results, the internal reference genes must exhibit stable expression across varied experimental conditions. Currently, no internal reference genes for <i<Camellia impressinervis</i< have been established. This study aimed to identify stable internal reference genes from eight candidates derived from different developmental stages of <i<C. impressinervis</i< flowers. We employed geNorm, NormFinder, and BestKeeper to evaluate the expression stability of these candidates, which was followed by a comprehensive stability analysis. The results indicated that <i<CiTUB</i<, a tubulin gene, exhibited the most stable expression among the eight reference gene candidates in the petals. Subsequently, <i<CiTUB</i< was utilized as an internal reference for the qRT-PCR analysis of six genes implicated in the petal pigment synthesis pathway of <i<C. impressinervis</i<. The qRT-PCR results were corroborated by transcriptome sequencing data, affirming the stability and suitability of <i<CiTUB</i< as a reference gene. This study marks the first identification of stable internal reference genes within the entire genome of <i<C. impressinervis</i<, establishing a foundation for future gene expression and functional studies. Identifying such stable reference genes is crucial for advancing molecular research on <i<C. impressinervis</i<. | ||
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| 700 | 0 | |a Yuqing Lin |e verfasserin |4 aut | |
| 700 | 0 | |a Xinyu Xu |e verfasserin |4 aut | |
| 700 | 0 | |a Zhongjian Liu |e verfasserin |4 aut | |
| 700 | 0 | |a Shuangquan Zou |e verfasserin |4 aut | |
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