Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in <i<Acheta domesticus</i<
The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks—DSB, 8-hydroxy-2′-deoxyguanosine-8-OHdG, abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism <i<Acheta domesticus</i<. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms..
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2022 |
---|---|
Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
---|---|
Enthalten in: |
International Journal of Molecular Sciences - 24(2022), 1, p 290 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Barbara Flasz [VerfasserIn] |
---|
Links: |
doi.org [kostenfrei] |
---|
Themen: |
8-OHdG |
---|
doi: |
10.3390/ijms24010290 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
DOAJ082614652 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | DOAJ082614652 | ||
003 | DE-627 | ||
005 | 20240413071153.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230311s2022 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3390/ijms24010290 |2 doi | |
035 | |a (DE-627)DOAJ082614652 | ||
035 | |a (DE-599)DOAJ9ac9779cd70c47fab1cea9b24a36bf45 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
050 | 0 | |a QH301-705.5 | |
050 | 0 | |a QD1-999 | |
100 | 0 | |a Barbara Flasz |e verfasserin |4 aut | |
245 | 1 | 0 | |a Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in <i<Acheta domesticus</i< |
264 | 1 | |c 2022 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks—DSB, 8-hydroxy-2′-deoxyguanosine-8-OHdG, abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism <i<Acheta domesticus</i<. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms. | ||
650 | 4 | |a total DNA damage | |
650 | 4 | |a DSB | |
650 | 4 | |a 8-OHdG | |
650 | 4 | |a AP sites | |
650 | 4 | |a pATM | |
650 | 4 | |a pH2A.X | |
653 | 0 | |a Biology (General) | |
653 | 0 | |a Chemistry | |
700 | 0 | |a Marta Dziewięcka |e verfasserin |4 aut | |
700 | 0 | |a Amrendra K. Ajay |e verfasserin |4 aut | |
700 | 0 | |a Monika Tarnawska |e verfasserin |4 aut | |
700 | 0 | |a Agnieszka Babczyńska |e verfasserin |4 aut | |
700 | 0 | |a Andrzej Kędziorski |e verfasserin |4 aut | |
700 | 0 | |a Łukasz Napora-Rutkowski |e verfasserin |4 aut | |
700 | 0 | |a Patrycja Ziętara |e verfasserin |4 aut | |
700 | 0 | |a Ewa Świerczek |e verfasserin |4 aut | |
700 | 0 | |a Maria Augustyniak |e verfasserin |4 aut | |
773 | 0 | 8 | |i In |t International Journal of Molecular Sciences |d MDPI AG, 2003 |g 24(2022), 1, p 290 |w (DE-627)DOAJ00014584X |x 14220067 |7 nnns |
773 | 1 | 8 | |g volume:24 |g year:2022 |g number:1, p 290 |
856 | 4 | 0 | |u https://doi.org/10.3390/ijms24010290 |z kostenfrei |
856 | 4 | 0 | |u https://doaj.org/article/9ac9779cd70c47fab1cea9b24a36bf45 |z kostenfrei |
856 | 4 | 0 | |u https://www.mdpi.com/1422-0067/24/1/290 |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/1661-6596 |y Journal toc |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/1422-0067 |y Journal toc |z kostenfrei |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_DOAJ | ||
951 | |a AR | ||
952 | |d 24 |j 2022 |e 1, p 290 |