Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone
Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (<i<Hdh-CAT</i<) in stress physiology, innate immunity, testicular development, metamorphosis, and cryopreserved sperm of Pacific abalone. <i<Hdh-CAT</i< gene was cloned from the digestive gland (DG) of Pacific abalone. The 2894 bp sequence of <i<Hdh-CAT</i< had an open reading frame of 1506 bp encoding 501 deduced amino acids. Fluorescence <i<in situ</i< hybridization confirmed <i<Hdh-CAT</i< localization in the digestive tubules of the DG. <i<Hdh-CAT</i< was induced by different types of stress including thermal stress, H<sub<2</sub<O<sub<2</sub< induction, and starvation. Immune challenges with <i<Vibrio</i<, lipopolysaccharides, and polyinosinic–polycytidylic acid sodium salt also upregulated <i<Hdh-CAT</i< mRNA expression and catalase activity. <i<Hdh-CAT</i< responded to cadmium induced-toxicity by increasing mRNA expression and catalase activity. Elevated seasonal temperature also altered <i<Hdh-CAT</i< mRNA expression. <i<Hdh-CAT</i< mRNA expression was relatively higher at the trochophore larvae stage of metamorphosis. Cryopreserved sperm showed significantly lower <i<Hdh-CAT</i< mRNA expression levels compared with fresh sperm. <i<Hdh-CAT</i< mRNA expression showed a relationship with the production of ROS. These results suggest that <i<Hdh-CAT</i< might play a role in stress physiology, innate immunity, testicular development, metamorphosis, and sperm cryo-tolerance of Pacific abalone..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Antioxidants - 12(2023), 1, p 109 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shaharior Hossen [VerfasserIn] |
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| Links: |
doi.org [kostenfrei] |
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| Themen: |
Catalase |
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| doi: |
10.3390/antiox12010109 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
DOAJ081858809 |
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| 520 | |a Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (<i<Hdh-CAT</i<) in stress physiology, innate immunity, testicular development, metamorphosis, and cryopreserved sperm of Pacific abalone. <i<Hdh-CAT</i< gene was cloned from the digestive gland (DG) of Pacific abalone. The 2894 bp sequence of <i<Hdh-CAT</i< had an open reading frame of 1506 bp encoding 501 deduced amino acids. Fluorescence <i<in situ</i< hybridization confirmed <i<Hdh-CAT</i< localization in the digestive tubules of the DG. <i<Hdh-CAT</i< was induced by different types of stress including thermal stress, H<sub<2</sub<O<sub<2</sub< induction, and starvation. Immune challenges with <i<Vibrio</i<, lipopolysaccharides, and polyinosinic–polycytidylic acid sodium salt also upregulated <i<Hdh-CAT</i< mRNA expression and catalase activity. <i<Hdh-CAT</i< responded to cadmium induced-toxicity by increasing mRNA expression and catalase activity. Elevated seasonal temperature also altered <i<Hdh-CAT</i< mRNA expression. <i<Hdh-CAT</i< mRNA expression was relatively higher at the trochophore larvae stage of metamorphosis. Cryopreserved sperm showed significantly lower <i<Hdh-CAT</i< mRNA expression levels compared with fresh sperm. <i<Hdh-CAT</i< mRNA expression showed a relationship with the production of ROS. These results suggest that <i<Hdh-CAT</i< might play a role in stress physiology, innate immunity, testicular development, metamorphosis, and sperm cryo-tolerance of Pacific abalone. | ||
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