Helicobacter pylori infected gastric epithelial cells bypass cell death pathway through the oncoprotein Gankyrin
H. pylori infection can lead to gastric diseases by modulating the various cellular processes such as cellular stress, apoptosis, autophagy, and metabolic changes. H. pylori exposed gastric epithelial cells bypass the cell death pathways. However, the underlying molecular mechanisms remain in infancy. Herein, we determined that H. pylori infection on gastric epithelial cells bypass the cell death pathway via the modulation of autophagy-related signaling molecules (LC3B and ATG7) through the host-associated oncoprotein Gankyrin. Upregulated expression of Gankyrin further enhanced the various antioxidant (gclm, gclc, sod2, cat, keap1, ant, and hsf1) and autophagy-associated genes’ transcripts (atg5, atg7, lc3b, beclin, and sqstm1). Elevated expression of Gankyrin also modulates the various downstream signaling proteins such as Akt, Beta-catenin, and NFkB. We also observed altered cancerous properties of gastric epithelial cells viz; apoptosis, wound healing, chemoresistance, biomass and membrane potential of mitochondria. Concisely, the study revealed that H. pylori infection promotes GC via autophagy through the modulation of oncoprotein Gankyrin and cellular reactive oxygen species (ROS). Overall, our study demonstrated the antiapoptotic property of H pylori-infected gastric epithelial cells might govern through Gankyrin-directed autophagy..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
|---|---|
| Enthalten in: |
Advances in Cancer Biology - Metastasis - 7(2023), Seite 100087- |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Dharmendra Kashyap [VerfasserIn] |
|---|
| Links: |
doi.org [kostenfrei] |
|---|
| Themen: |
Autophagy |
|---|
| doi: |
10.1016/j.adcanc.2023.100087 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
DOAJ081670443 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | DOAJ081670443 | ||
| 003 | DE-627 | ||
| 005 | 20230502062916.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230310s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.adcanc.2023.100087 |2 doi | |
| 035 | |a (DE-627)DOAJ081670443 | ||
| 035 | |a (DE-599)DOAJa43536bdb0c8412da3934aab872a3511 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 050 | 0 | |a RC254-282 | |
| 100 | 0 | |a Dharmendra Kashyap |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Helicobacter pylori infected gastric epithelial cells bypass cell death pathway through the oncoprotein Gankyrin |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a H. pylori infection can lead to gastric diseases by modulating the various cellular processes such as cellular stress, apoptosis, autophagy, and metabolic changes. H. pylori exposed gastric epithelial cells bypass the cell death pathways. However, the underlying molecular mechanisms remain in infancy. Herein, we determined that H. pylori infection on gastric epithelial cells bypass the cell death pathway via the modulation of autophagy-related signaling molecules (LC3B and ATG7) through the host-associated oncoprotein Gankyrin. Upregulated expression of Gankyrin further enhanced the various antioxidant (gclm, gclc, sod2, cat, keap1, ant, and hsf1) and autophagy-associated genes’ transcripts (atg5, atg7, lc3b, beclin, and sqstm1). Elevated expression of Gankyrin also modulates the various downstream signaling proteins such as Akt, Beta-catenin, and NFkB. We also observed altered cancerous properties of gastric epithelial cells viz; apoptosis, wound healing, chemoresistance, biomass and membrane potential of mitochondria. Concisely, the study revealed that H. pylori infection promotes GC via autophagy through the modulation of oncoprotein Gankyrin and cellular reactive oxygen species (ROS). Overall, our study demonstrated the antiapoptotic property of H pylori-infected gastric epithelial cells might govern through Gankyrin-directed autophagy. | ||
| 650 | 4 | |a Helicobacter pylori | |
| 650 | 4 | |a Autophagy | |
| 650 | 4 | |a Gankyrin | |
| 650 | 4 | |a Cell-signaling | |
| 650 | 4 | |a ROS | |
| 650 | 4 | |a Mitochondrial membrane potential | |
| 653 | 0 | |a Neoplasms. Tumors. Oncology. Including cancer and carcinogens | |
| 700 | 0 | |a Nidhi Varshney |e verfasserin |4 aut | |
| 700 | 0 | |a Budhadev Baral |e verfasserin |4 aut | |
| 700 | 0 | |a Meenakshi Kandpal |e verfasserin |4 aut | |
| 700 | 0 | |a Omkar Indari |e verfasserin |4 aut | |
| 700 | 0 | |a Ajay Kumar Jain |e verfasserin |4 aut | |
| 700 | 0 | |a Debi Chatterji |e verfasserin |4 aut | |
| 700 | 0 | |a Sachin Kumar |e verfasserin |4 aut | |
| 700 | 0 | |a Hamendra Singh Parmar |e verfasserin |4 aut | |
| 700 | 0 | |a Avinash Sonawane |e verfasserin |4 aut | |
| 700 | 0 | |a Hem Chandra Jha |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i In |t Advances in Cancer Biology - Metastasis |d Elsevier, 2021 |g 7(2023), Seite 100087- |w (DE-627)DOAJ078627745 |x 26673940 |7 nnns |
| 773 | 1 | 8 | |g volume:7 |g year:2023 |g pages:100087- |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.adcanc.2023.100087 |z kostenfrei |
| 856 | 4 | 0 | |u https://doaj.org/article/a43536bdb0c8412da3934aab872a3511 |z kostenfrei |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S2667394023000011 |z kostenfrei |
| 856 | 4 | 2 | |u https://doaj.org/toc/2667-3940 |y Journal toc |z kostenfrei |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_DOAJ | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |d 7 |j 2023 |h 100087- | ||