Culturing the Chicken Intestinal Microbiota and Potential Application as Probiotics Development
Pure cultures of chicken intestinal microbial species may still be crucial and imperative to expound on the function of gut microbiota, and also contribute to the development of potential probiotics and novel bioactive metabolites from gut microbiota. In this study, we isolated and identified 507 chicken intestinal bacterial isolates, including 89 previously uncultured isolates. Among these, a total of 63 <i<Lactobacillus</i< strains, belonging to <i<L. vaginalis</i<, <i<L. crispatus</i<, <i<L. gallinarum</i<, <i<L. reuteri</i<, <i<L. salivarius</i<, and <i<L. saerimneri</i<, exhibited antibacterial activity against <i<S. Pullorum</i<. Acid tolerance tests showed <i<Limosilactobacillus reuteri</i< strain YPG14 (<i<L. reuteri</i< strain YPG14) has a particularly strong tolerance to acid. We further characterized other probiotic properties of <i<L. reuteri</i< strain YPG14. In simulated intestinal fluid, the growth of <i<L. reuteri</i< strain YPG14 remained stable after incubation for 4 h. The auto-aggregation test showed the auto-aggregation percentage of <i<L. reuteri</i< strain YPG14 was recorded as 15.0 ± 0.38%, 48.3 ± 2.51%, and 75.1 ± 4.44% at 3, 12, and 24 h, respectively. In addition, the mucin binding assay showed <i<L. reuteri</i< strain YPG14 exhibited 12.07 ± 0.02% adhesion to mucin. Antibiotic sensitivity testing showed that <i<L. reuteri</i< strain YPG14 was sensitive to the majority of the tested antibiotics. The anti-<i<Salmonella</i< Pullorum (<i<S</i<. Pullorum) infection effect in vivo revealed that the consumption of <i<L. reuteri</i< strain YPG14 could significantly improve body weight loss and survival rate of chicks infected by <i<S</i<. Pullorum; reduce the loads of <i<S</i<. Pullorum in the jejunum, liver, spleen, and feces; and alleviate the jejunum villi morphological structure damage, crypt loss, and inflammatory cell infiltration caused by <i<S</i<. Pullorum. Overall, this study may help us to understand the diversity of chicken intestinal microflora and provide some insights for potential probiotic development from gut microbiota and may find application in the poultry industry..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
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| Enthalten in: |
International Journal of Molecular Sciences - 24(2023), 3, p 3045 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ke Ma [VerfasserIn] |
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| Links: |
doi.org [kostenfrei] |
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| Themen: |
Anti- |
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| doi: |
10.3390/ijms24033045 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
DOAJ080630553 |
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| 520 | |a Pure cultures of chicken intestinal microbial species may still be crucial and imperative to expound on the function of gut microbiota, and also contribute to the development of potential probiotics and novel bioactive metabolites from gut microbiota. In this study, we isolated and identified 507 chicken intestinal bacterial isolates, including 89 previously uncultured isolates. Among these, a total of 63 <i<Lactobacillus</i< strains, belonging to <i<L. vaginalis</i<, <i<L. crispatus</i<, <i<L. gallinarum</i<, <i<L. reuteri</i<, <i<L. salivarius</i<, and <i<L. saerimneri</i<, exhibited antibacterial activity against <i<S. Pullorum</i<. Acid tolerance tests showed <i<Limosilactobacillus reuteri</i< strain YPG14 (<i<L. reuteri</i< strain YPG14) has a particularly strong tolerance to acid. We further characterized other probiotic properties of <i<L. reuteri</i< strain YPG14. In simulated intestinal fluid, the growth of <i<L. reuteri</i< strain YPG14 remained stable after incubation for 4 h. The auto-aggregation test showed the auto-aggregation percentage of <i<L. reuteri</i< strain YPG14 was recorded as 15.0 ± 0.38%, 48.3 ± 2.51%, and 75.1 ± 4.44% at 3, 12, and 24 h, respectively. In addition, the mucin binding assay showed <i<L. reuteri</i< strain YPG14 exhibited 12.07 ± 0.02% adhesion to mucin. Antibiotic sensitivity testing showed that <i<L. reuteri</i< strain YPG14 was sensitive to the majority of the tested antibiotics. The anti-<i<Salmonella</i< Pullorum (<i<S</i<. Pullorum) infection effect in vivo revealed that the consumption of <i<L. reuteri</i< strain YPG14 could significantly improve body weight loss and survival rate of chicks infected by <i<S</i<. Pullorum; reduce the loads of <i<S</i<. Pullorum in the jejunum, liver, spleen, and feces; and alleviate the jejunum villi morphological structure damage, crypt loss, and inflammatory cell infiltration caused by <i<S</i<. Pullorum. Overall, this study may help us to understand the diversity of chicken intestinal microflora and provide some insights for potential probiotic development from gut microbiota and may find application in the poultry industry. | ||
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| 700 | 0 | |a Xiao-Qi Lin |e verfasserin |4 aut | |
| 700 | 0 | |a Zhen-Zhen Liu |e verfasserin |4 aut | |
| 700 | 0 | |a Tao Wang |e verfasserin |4 aut | |
| 700 | 0 | |a Jia-Bao Zhang |e verfasserin |4 aut | |
| 700 | 0 | |a Jian-Gang Zhang |e verfasserin |4 aut | |
| 700 | 0 | |a Cheng-Kai Zhou |e verfasserin |4 aut | |
| 700 | 0 | |a Yu Gao |e verfasserin |4 aut | |
| 700 | 0 | |a Chong-Tao Du |e verfasserin |4 aut | |
| 700 | 0 | |a Yong-Jun Yang |e verfasserin |4 aut | |
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