Comparison of Two Different Methods for Determination of hVISA Rates in Methicilline-Resistant Staphylococcus aureus
INTRODUCTION: EExcept vancomycin-resistant Staphylococcus aures (VRSA) and vancomycin intermediate S. aureus (VISA), one of the important causes of failure in the treatment of staphylococcal infections is considered to be vancomycin heterogeneous-intermediate resistant S. aureus (hVISA). But detecting hVISA is a labor-intensive and specialized procedure. In our study, it was aimed to determine the hVISA rates in Methicilline-Resistant Staphylococcus aureus (MRSA) strains isolated from a tertiary university hospital and to compare the population analysis profile (PAP) method with the Satola test METHODS: Sixty MRSA isolates were taken to the study. First of all, the MIC values of all isolates were determined using the broth microdilution method. Then the Brain Heart Infusion agar screening method developed by Satola and the PAP method, which is accepted as the gold standard, were compared for detecting hVISA. RESULTS: All isolates were found to be sensitive to vancomycin. Satola method was perfomed and 28 isolates (46.66%) were determined as hVISA at the 48th hour. These strains detected as hVISA were re-evaluated in the PAP method. Among these only 20% (12/60 of all isolates) strains were identified as hVISA. DISCUSSION AND CONCLUSION: In our study, hVISA rates were investigated for the first time in our region using Satola's method and PAP method. In the light of the data obtained, when the Satola test and PAP were compared, it was determined that the false positive rate was high in the Satola test..
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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Enthalten in: |
Van Tıp Dergisi - 30(2023), 1, Seite 86-93 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Nesrin Sakarya [VerfasserIn] |
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Links: |
doi.org [kostenfrei] |
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Themen: |
H-visa |
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doi: |
10.5505/vtd.2023.47639 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
DOAJ080173616 |
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