Protective mechanism of punicalagin against endoplasmic reticulum stress in the liver of mice with type 2 diabetes mellitus
To develop a more effective and safer drug for the treatment of type 2 diabetes mellitus (T2DM)-induced liver damage, we investigated the protective effects of punicalagin, a major component in pomegranate peel, on the liver of mice with T2DM. After five weeks of punicalagin treatment, blood and liver samples were obtained for the subsequent analyses. Western blotting, reverse transcription polymerase chain reaction, and immunohistochemical staining were performed to determine the expression of endoplasmic reticulum (ER) stress markers in the liver tissue. The results showed that punicalagin alleviated glucose and insulin resistance, increased insulin sensitivity, and reduced serum free fatty acids levels and hepatic steatosis in the mice with T2DM. Furthermore, punicalagin down-regulated the elevated mRNA expression of the ER stress markers eIF2α, GRP78, ATF4, and CHOP in the liver of mice with T2DM. Our results suggest that punicalagin is a potential natural agent for the prevention of T2DM-induced liver damage..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:56 |
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| Enthalten in: |
Journal of Functional Foods - 56(2019), Seite 57-64 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fang-fang Mo [VerfasserIn] |
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| Links: |
doi.org [kostenfrei] |
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| Themen: |
Endoplasmic reticulum stress |
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| doi: |
10.1016/j.jff.2019.03.006 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
DOAJ062228099 |
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| 520 | |a To develop a more effective and safer drug for the treatment of type 2 diabetes mellitus (T2DM)-induced liver damage, we investigated the protective effects of punicalagin, a major component in pomegranate peel, on the liver of mice with T2DM. After five weeks of punicalagin treatment, blood and liver samples were obtained for the subsequent analyses. Western blotting, reverse transcription polymerase chain reaction, and immunohistochemical staining were performed to determine the expression of endoplasmic reticulum (ER) stress markers in the liver tissue. The results showed that punicalagin alleviated glucose and insulin resistance, increased insulin sensitivity, and reduced serum free fatty acids levels and hepatic steatosis in the mice with T2DM. Furthermore, punicalagin down-regulated the elevated mRNA expression of the ER stress markers eIF2α, GRP78, ATF4, and CHOP in the liver of mice with T2DM. Our results suggest that punicalagin is a potential natural agent for the prevention of T2DM-induced liver damage. | ||
| 650 | 4 | |a Punicalagin | |
| 650 | 4 | |a Endoplasmic reticulum stress | |
| 650 | 4 | |a Liver | |
| 650 | 4 | |a Type 2 diabetes mellitus | |
| 650 | 4 | |a Mice | |
| 653 | 0 | |a Nutrition. Foods and food supply | |
| 700 | 0 | |a Bo-han Lv |e verfasserin |4 aut | |
| 700 | 0 | |a Tian An |e verfasserin |4 aut | |
| 700 | 0 | |a Jia-nan Miao |e verfasserin |4 aut | |
| 700 | 0 | |a Jia-xian Liu |e verfasserin |4 aut | |
| 700 | 0 | |a Jing Zhang |e verfasserin |4 aut | |
| 700 | 0 | |a Zhi-yong Zhang |e verfasserin |4 aut | |
| 700 | 0 | |a Meng-hua Ma |e verfasserin |4 aut | |
| 700 | 0 | |a Xiu-yan Yang |e verfasserin |4 aut | |
| 700 | 0 | |a Dan-dan Zhao |e verfasserin |4 aut | |
| 700 | 0 | |a Dong-wei Zhang |e verfasserin |4 aut | |
| 700 | 0 | |a Si-hua Gao |e verfasserin |4 aut | |
| 700 | 0 | |a Guang-jian Jiang |e verfasserin |4 aut | |
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