Quantitative analysis of m6A RNA modification by LC-MS
Summary: N6-adenosine methylation (m6A) of messenger RNA (mRNA) plays key regulatory roles in gene expression. Accurate measurement of m6A levels is thus critical to understand its dynamic changes in various biological settings. Here, we provide a protocol to quantitate the levels of adenosine and m6A in cellular mRNAs. Using nuclease and phosphatase, we digest mRNA into nucleosides, which are subsequently quantified using liquid chromatography mass spectrometry.For complete details on the use and execution of this protocol, please refer to Cho et al. (2021)..
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
---|---|
Enthalten in: |
STAR Protocols - 2(2021), 3, Seite 100724- |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Lavina Mathur [VerfasserIn] |
---|
Links: |
doi.org [kostenfrei] |
---|
Themen: |
Cell biology |
---|
doi: |
10.1016/j.xpro.2021.100724 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
DOAJ057735026 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | DOAJ057735026 | ||
003 | DE-627 | ||
005 | 20230308215814.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230227s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.xpro.2021.100724 |2 doi | |
035 | |a (DE-627)DOAJ057735026 | ||
035 | |a (DE-599)DOAJ7aab75337f574404a8d757554d06c875 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
050 | 0 | |a Q1-390 | |
100 | 0 | |a Lavina Mathur |e verfasserin |4 aut | |
245 | 1 | 0 | |a Quantitative analysis of m6A RNA modification by LC-MS |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Summary: N6-adenosine methylation (m6A) of messenger RNA (mRNA) plays key regulatory roles in gene expression. Accurate measurement of m6A levels is thus critical to understand its dynamic changes in various biological settings. Here, we provide a protocol to quantitate the levels of adenosine and m6A in cellular mRNAs. Using nuclease and phosphatase, we digest mRNA into nucleosides, which are subsequently quantified using liquid chromatography mass spectrometry.For complete details on the use and execution of this protocol, please refer to Cho et al. (2021). | ||
650 | 4 | |a cell biology | |
650 | 4 | |a cell culture | |
650 | 4 | |a metabolism | |
650 | 4 | |a molecular biology | |
650 | 4 | |a mass spectrometry | |
650 | 4 | |a chemistry | |
653 | 0 | |a Science (General) | |
700 | 0 | |a Sunhee Jung |e verfasserin |4 aut | |
700 | 0 | |a Cholsoon Jang |e verfasserin |4 aut | |
700 | 0 | |a Gina Lee |e verfasserin |4 aut | |
773 | 0 | 8 | |i In |t STAR Protocols |d Elsevier, 2020 |g 2(2021), 3, Seite 100724- |w (DE-627)DOAJ078605954 |x 26661667 |7 nnns |
773 | 1 | 8 | |g volume:2 |g year:2021 |g number:3 |g pages:100724- |
856 | 4 | 0 | |u https://doi.org/10.1016/j.xpro.2021.100724 |z kostenfrei |
856 | 4 | 0 | |u https://doaj.org/article/7aab75337f574404a8d757554d06c875 |z kostenfrei |
856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S2666166721004317 |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/2666-1667 |y Journal toc |z kostenfrei |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_DOAJ | ||
951 | |a AR | ||
952 | |d 2 |j 2021 |e 3 |h 100724- |