Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways
Icaritin, a prenylflavonoid isolated from Herbal Epimedii, is well known for its osteoplastic and anti-tumour activities. Here, we examined the cardioprotective actions of icaritin on cardiac H9c2 cells under oxidative stress conditions induced by treatment with tert-butylhydroperoxide (t-BHP). Icaritin at a concentration range between 1 and 4 µM effectively increased cell viability and decreased lactate dehydrogenase (LDH) leakage and reactive oxygen species (ROS) release in t-BHP treated cells, reflecting its ability to reduce t-BHP-induced cell injury. Icaritin also protected cell membrane integrity by preventing the collapse of the mitochondrial membrane potential. In addition, pretreatment of NF-E2-related factor 2 (Nrf2) siRNA and Akt inhibitor abolished the cardioprotective effect of icaritin. Furthermore, Nrf2 siRNA transfection interfered with the effect of icaritin on the inhibition of Ca2+ overload, whereas Akt inhibitor reduced icaritin-induced haemo-oxygenase-1 (HO-1) expression. Collectively, these results suggest that icaritin exerted its cardioprotective effect through the Akt/Nrf2/HO-1 and calcium signalling pathways..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Journal of Functional Foods - 18(2015), Seite 213-223 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Si Wan Lei [VerfasserIn] |
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| Links: |
doi.org [kostenfrei] |
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| Themen: |
Calcium ion |
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| doi: |
10.1016/j.jff.2015.06.054 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
DOAJ054969689 |
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| 520 | |a Icaritin, a prenylflavonoid isolated from Herbal Epimedii, is well known for its osteoplastic and anti-tumour activities. Here, we examined the cardioprotective actions of icaritin on cardiac H9c2 cells under oxidative stress conditions induced by treatment with tert-butylhydroperoxide (t-BHP). Icaritin at a concentration range between 1 and 4 µM effectively increased cell viability and decreased lactate dehydrogenase (LDH) leakage and reactive oxygen species (ROS) release in t-BHP treated cells, reflecting its ability to reduce t-BHP-induced cell injury. Icaritin also protected cell membrane integrity by preventing the collapse of the mitochondrial membrane potential. In addition, pretreatment of NF-E2-related factor 2 (Nrf2) siRNA and Akt inhibitor abolished the cardioprotective effect of icaritin. Furthermore, Nrf2 siRNA transfection interfered with the effect of icaritin on the inhibition of Ca2+ overload, whereas Akt inhibitor reduced icaritin-induced haemo-oxygenase-1 (HO-1) expression. Collectively, these results suggest that icaritin exerted its cardioprotective effect through the Akt/Nrf2/HO-1 and calcium signalling pathways. | ||
| 650 | 4 | |a Icaritin | |
| 650 | 4 | |a Cardiac H9c2 cells | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 4 | |a NF-E2-related factor 2 | |
| 650 | 4 | |a Calcium ion | |
| 653 | 0 | |a Nutrition. Foods and food supply | |
| 700 | 0 | |a Guozhen Cui |e verfasserin |4 aut | |
| 700 | 0 | |a George Pak Heng Leung |e verfasserin |4 aut | |
| 700 | 0 | |a Sharon Chui Wah Luk |e verfasserin |4 aut | |
| 700 | 0 | |a Maggie Pui Man Hoi |e verfasserin |4 aut | |
| 700 | 0 | |a Liang Wang |e verfasserin |4 aut | |
| 700 | 0 | |a Gail B. Mahady |e verfasserin |4 aut | |
| 700 | 0 | |a Simon Ming Yuen Lee |e verfasserin |4 aut | |
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