Determination of bioanalytical parameters for the standardization of Abroma augusta
Objective: To set the analytical parameters for simultaneous determination of different phytochemicals present in the Abroma augusta (A. augusta). Methods: Different phytochemical test including total phenol and total flavonoid content were carried out in the present investigation. Further, a rapid and simple high-performance thin-layer chromatographic (HPTLC) method was developed for the qualitative and quantitative analysis. Moreover antimicrobial study was also performed in the present investigation to know the presence of biological contamination. Results: Preliminary phytochemical analysis revealed the presence of glycoside, alkaloid, carbohydrate and steroid in the A. augusta. Total phenol and total flavonoid content were found to be 0.29% and 0.50% w/w respectively. All tested microorganism in the A. augusta extract were found to be under the limit. Chloroform: methanol: acetic acid: H2O (170:25:25:4) was used as a solvent system for the qualitative and quantitative analysis through HPTLC Method. HPTLC analysis revealed 1.87 % w/w of linoleic acid in the A. augusta extract. Conclusions: Results showed that the selected parameters in the present investigation would set the new standards for the qualitative and quantitative analysis of A. augusta..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
|---|---|
| Enthalten in: |
Journal of Acute Disease - 2(2013), 4, Seite 292-295 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dinesh K. Patel [VerfasserIn] |
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| Links: |
doi.org [kostenfrei] |
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| Themen: |
Abroma augusta |
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| doi: |
10.1016/S2221-6189(13)60146-8 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
DOAJ043878814 |
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| 520 | |a Objective: To set the analytical parameters for simultaneous determination of different phytochemicals present in the Abroma augusta (A. augusta). Methods: Different phytochemical test including total phenol and total flavonoid content were carried out in the present investigation. Further, a rapid and simple high-performance thin-layer chromatographic (HPTLC) method was developed for the qualitative and quantitative analysis. Moreover antimicrobial study was also performed in the present investigation to know the presence of biological contamination. Results: Preliminary phytochemical analysis revealed the presence of glycoside, alkaloid, carbohydrate and steroid in the A. augusta. Total phenol and total flavonoid content were found to be 0.29% and 0.50% w/w respectively. All tested microorganism in the A. augusta extract were found to be under the limit. Chloroform: methanol: acetic acid: H2O (170:25:25:4) was used as a solvent system for the qualitative and quantitative analysis through HPTLC Method. HPTLC analysis revealed 1.87 % w/w of linoleic acid in the A. augusta extract. Conclusions: Results showed that the selected parameters in the present investigation would set the new standards for the qualitative and quantitative analysis of A. augusta. | ||
| 650 | 4 | |a Abroma augusta | |
| 650 | 4 | |a HPTLC | |
| 650 | 4 | |a Phytochemical analysis | |
| 650 | 4 | |a Standardization | |
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