In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from <em<Culicoides</em<
Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this study, a strain of mosquito flavivirus (MFV) YN15-283-02 was detected in <i<Culicoides</i< collected from Yunnan, China. The isolation of the purified MFV YN15-283-02 from cell culture failed, and the virus was then rescued by an infectious clone. To study the biological features of MFV YN15-283-02 in vitro and in vivo, electron microscopy, phylogenetic tree, and viral growth kinetic analyses were performed in both cell lines and mosquitoes. The rescued MFV (rMFV) YN15-283-02 duplicated and reached a peak in C6/36 cells at 6 d.p.i. with approximately 2 × 10<sup<6</sup< RNA copies/μL (RNA to cell ratio of 0.1), but without displaying a cytopathic effect. In addition, the infection rate for the rMFV in <i<Ae.</i<<i<aegypti</i< show a low level in both larvae (≤15%) and adult mosquitoes (≤12%)..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Viruses - 14(2022), 6, p 1298 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yi Huang [VerfasserIn] |
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| Links: |
doi.org [kostenfrei] |
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| Themen: |
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| doi: |
10.3390/v14061298 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
DOAJ02732141X |
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| 520 | |a Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this study, a strain of mosquito flavivirus (MFV) YN15-283-02 was detected in <i<Culicoides</i< collected from Yunnan, China. The isolation of the purified MFV YN15-283-02 from cell culture failed, and the virus was then rescued by an infectious clone. To study the biological features of MFV YN15-283-02 in vitro and in vivo, electron microscopy, phylogenetic tree, and viral growth kinetic analyses were performed in both cell lines and mosquitoes. The rescued MFV (rMFV) YN15-283-02 duplicated and reached a peak in C6/36 cells at 6 d.p.i. with approximately 2 × 10<sup<6</sup< RNA copies/μL (RNA to cell ratio of 0.1), but without displaying a cytopathic effect. In addition, the infection rate for the rMFV in <i<Ae.</i<<i<aegypti</i< show a low level in both larvae (≤15%) and adult mosquitoes (≤12%). | ||
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| 700 | 0 | |a Xiaodan Li |e verfasserin |4 aut | |
| 700 | 0 | |a Lu Zhao |e verfasserin |4 aut | |
| 700 | 0 | |a Dirui Cai |e verfasserin |4 aut | |
| 700 | 0 | |a Shunlong Wang |e verfasserin |4 aut | |
| 700 | 0 | |a Nanjie Ren |e verfasserin |4 aut | |
| 700 | 0 | |a Haixia Ma |e verfasserin |4 aut | |
| 700 | 0 | |a Doudou Huang |e verfasserin |4 aut | |
| 700 | 0 | |a Fei Wang |e verfasserin |4 aut | |
| 700 | 0 | |a Zhiming Yuan |e verfasserin |4 aut | |
| 700 | 0 | |a Bo Zhang |e verfasserin |4 aut | |
| 700 | 0 | |a Han Xia |e verfasserin |4 aut | |
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