Adenovirus-mediated wild-type PTEN promoting glioma stem/progenitor cellsautophagy activity
<strong<Background</strong< PTEN is an anti-oncogene frequently inactivating in glioma. The previous study found that <em<PTEN</em< was closely related to cellular autophagy activity. The purpose of this paper is to study whether the inactivation of <em<PTEN</em< in glioma stem/progenitor cells (GSPCs) is correlative<br /<with the low autophagic activity in GSPCs. <strong<Methods</strong< Wild-type <em<PTEN</em< genes were transferred into GSPCs mediated by adenovirus. The autophagic activity in GSPCs before or after the introduction of wild-type <em<PTEN</em< was detected by immunocytochemistry, electron microscopy, and Western blotting assay. <strong<Results</strong< After transfection of wild-type <em<PTEN</em<, a large number of microtuble-associated protein 1 light chain 3 (MAP1LC3)-positive granules could be found in the cytoplasm of GSPCs under a confocal microscopy, and these granules were demonstrated to be autophagosomes under an electron microscope. Moreover, the expression of autophagy-related gene <em<Beclin-1</em< significantly increased after the transfection of wild-type <em<PTEN</em< gene. <strong<Conclusion</strong< The inactivation of PTEN in GSPCs is one reason of the low autophagic activity of GSPCs. <br /<.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Chinese Journal of Contemporary Neurology and Neurosurgery - 13(2013), 5, Seite 435-440 |
| Sprache: |
Englisch ; Chinesisch |
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| Beteiligte Personen: |
Yao-dong ZHAO [VerfasserIn] |
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| Links: |
doaj.org [kostenfrei] |
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| Themen: |
Autophagy |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
DOAJ02598974X |
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| 245 | 1 | 0 | |a Adenovirus-mediated wild-type PTEN promoting glioma stem/progenitor cellsautophagy activity |
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| 520 | |a <strong<Background</strong< PTEN is an anti-oncogene frequently inactivating in glioma. The previous study found that <em<PTEN</em< was closely related to cellular autophagy activity. The purpose of this paper is to study whether the inactivation of <em<PTEN</em< in glioma stem/progenitor cells (GSPCs) is correlative<br /<with the low autophagic activity in GSPCs. <strong<Methods</strong< Wild-type <em<PTEN</em< genes were transferred into GSPCs mediated by adenovirus. The autophagic activity in GSPCs before or after the introduction of wild-type <em<PTEN</em< was detected by immunocytochemistry, electron microscopy, and Western blotting assay. <strong<Results</strong< After transfection of wild-type <em<PTEN</em<, a large number of microtuble-associated protein 1 light chain 3 (MAP1LC3)-positive granules could be found in the cytoplasm of GSPCs under a confocal microscopy, and these granules were demonstrated to be autophagosomes under an electron microscope. Moreover, the expression of autophagy-related gene <em<Beclin-1</em< significantly increased after the transfection of wild-type <em<PTEN</em< gene. <strong<Conclusion</strong< The inactivation of PTEN in GSPCs is one reason of the low autophagic activity of GSPCs. <br /< | ||
| 650 | 4 | |a Glioma | |
| 650 | 4 | |a Neoplastic stem cells | |
| 650 | 4 | |a Genes, tumor suppressor | |
| 650 | 4 | |a Transfection | |
| 650 | 4 | |a Autophagy | |
| 650 | 4 | |a Immunoblotting | |
| 653 | 0 | |a Neurology. Diseases of the nervous system | |
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| 700 | 0 | |a Quan-bin ZHANG |e verfasserin |4 aut | |
| 700 | 0 | |a Mei-qing LOU |e verfasserin |4 aut | |
| 700 | 0 | |a Qiang HUANG |e verfasserin |4 aut | |
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