Effect of melatonin on formation of 8-oxo-2’-deoxyguanosine under fenton reaction conditions
In this paper we studied the effect of melatonin at three concentrations on the formation of 8-oxo-2'-dioxyguanosine (8-oxo-dG) under the conditions of the Fenton reaction. Reproducible kinetic curve of 8-oxo-dG accumulation in this reaction was obtained by the method of reversedphase high performance liquid chromatography with amperometric detection. When adding melatonin in all three concentrations studied, there was a noticeable change in the shape of the kinetic curve with a significant decrease in the amount of detected 8-oxo-dG in most of its control points. This suggests the melatonin inhibition of 8-oxo-dG formation in the reaction and confirms the hypothesis of a direct, non-hormonal, antioxidant effect of melatonin. However, the dependence of the inhibitory effect of melatonin on its concentration is not a straight line. The higher of the studied concentrations of melatonin (60 mg/ml) gave the least pronounced effect..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
Тонкие химические технологии - 10(2015), 1, Seite 50-55 |
| Sprache: |
Russisch |
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| Beteiligte Personen: |
N. V. Marmiy [VerfasserIn] |
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| Links: |
doaj.org [kostenfrei] |
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| Themen: |
8-oxo-2'-deoxyguanosine, melatonin, fenton reaction, antioxidant effect, reversedphase hplc, electrochemical detection |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
DOAJ024318396 |
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| 520 | |a In this paper we studied the effect of melatonin at three concentrations on the formation of 8-oxo-2'-dioxyguanosine (8-oxo-dG) under the conditions of the Fenton reaction. Reproducible kinetic curve of 8-oxo-dG accumulation in this reaction was obtained by the method of reversedphase high performance liquid chromatography with amperometric detection. When adding melatonin in all three concentrations studied, there was a noticeable change in the shape of the kinetic curve with a significant decrease in the amount of detected 8-oxo-dG in most of its control points. This suggests the melatonin inhibition of 8-oxo-dG formation in the reaction and confirms the hypothesis of a direct, non-hormonal, antioxidant effect of melatonin. However, the dependence of the inhibitory effect of melatonin on its concentration is not a straight line. The higher of the studied concentrations of melatonin (60 mg/ml) gave the least pronounced effect. | ||
| 650 | 4 | |a 8-oxo-2'-deoxyguanosine, melatonin, fenton reaction, antioxidant effect, reversedphase hplc, electrochemical detection | |
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