A panel of oxidative stress assays does not provide supplementary diagnostic information in Behcet's disease patients

<p<Abstract</p< <p<Background</p< <p<Recent findings suggest a role of oxidative stress in the pathogenesis of Behcet's disease (BD), but the utility of oxidative stress-associated assays in offering diagnostic information or in the monitoring of disease activity is largely unassessed.</p< <p<Objective and methods</p< <p<We aimed to measure oxidative and inflammatory markers, along with the markers of reactive nitrogen species, S-nitrosothiols and 3-nitrotyrosine, in BD patients (n = 100) and healthy volunteers (n = 50). These markers were evaluated in regard to their role in the pathogenesis of BD as well as their relation to clinical presentation, disease activity and duration.</p< <p<Results</p< <p<Median values for erythrocyte sedimentation rate (ESR), C-reactive protein, leukocyte count, and IL-18 levels, as well as myeloperoxidase (MPO) activity, were statistically higher in the patient group compared to controls. Some inflammation markers (ESR, neutrophil and leukocyte counts) were statistically higher (p < 0.05) in the active period. In contrast, oxidative stress-associated measures (erythrocyte lipid peroxidation, antioxidant enzymes and measures of serum antioxidant capacity), revealed no statistically significant differences between the median values in BD patients versus healthy control subjects (p < 0.05 in all statistical comparisons), nor was there any difference in median levels of these oxidative stress markers in active disease versus disease remission. S-nitrosothiols and 3-nitrotyrosine were undetectable in BD plasma.</p< <p<Conclusions</p< <p<The application of oxidative stress-associated measures to BD blood samples offered no supplemental diagnostic or disease activity information to that provided by standard laboratory measures of inflammation. S-nitrosothiols and 3-nitrotyrosine appeared not to be markers for active BD; thus the search for biochemical markers that will indicate the active period should be continued with larger studies.</p<.

Medienart:	E-Artikel

Erscheinungsjahr:	2012
Erschienen:	2012

Enthalten in:	Zur Gesamtaufnahme - volume:9
Enthalten in:	Journal of Inflammation - 9(2012), 1, p 13

Sprache:	Englisch

Beteiligte Personen:	Akcay Yasemin D [VerfasserIn] Sagin Ferhan G [VerfasserIn] Aksu Kenan [VerfasserIn] Keser Gokhan [VerfasserIn] Taylor Emma [VerfasserIn] Knight Iona [VerfasserIn] Winyard Paul G [VerfasserIn] Sozmen Eser Y [VerfasserIn]

Links:	doi.org [kostenfrei] doaj.org [kostenfrei] www.journal-inflammation.com [kostenfrei] Journal toc [kostenfrei]

Themen:	Behçet's disease Cytokines Electron paramagnetic resonance spectrometry Inflammation Nitric oxide Nitrotyrosine Oxidative stress S-nitrosothiols Therapeutics. Pharmacology

doi:	10.1186/1476-9255-9-13

funding:
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PPN (Katalog-ID):	DOAJ021737126