SYNTHESIS OF 25-HYDROXYVITAMIN D3 CONJUGATE WITH KEYHOLE LIMPET HEMOCYANIN AND OBTAINING OF IMMUNE SERA
The study was aimed at obtaining polyclonal antibodies that recognize 25-Hydroxyvitamin D3, for their further specifications and applications in immunochemical test systems of 25-Hydroxyvitamin D3, determination in blood serum. We used the methods of chemical synthesis of immunoconjugates (modified carbodiimide method using N´-ethyl carbodiimide), thin layer chromatography, gel filtration and indirect immunoenzyme analysis ELISA. The work describes the stages of the synthesis of 25-Hydroxyvitamin D3, immunoconjugate with keyhole limpet hemocyanin (KLH), which was used for receiving immune sera. As a result of mouse and rabbit immunization antisera were obtained and antibody titers against 25-Hydroxyvitamin D3, were tested by immunoenzyme assay. It was demonstrated that the titer of specific antibodies was higher in rabbits compared with mice. The resulting polyclonal antibodies can be used for the development of immunochemical test systems for screening studies of 25-Hydroxyvitamin D3, content in human blood serum as a marker of vitamin D3 availability..
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2015 |
---|---|
Erschienen: |
2015 |
Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
---|---|
Enthalten in: |
Biotechnologia Acta - 8(2015), 3, Seite 45-55 |
Sprache: |
Englisch ; Russisch ; Ukrainisch |
---|
Beteiligte Personen: |
А. O. Mazanova [VerfasserIn] |
---|
Links: |
doi.org [kostenfrei] |
---|
Themen: |
25-Hydroxyvitamin D3 |
---|
doi: |
10.15407/biotech8.03.045 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
DOAJ018094791 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | DOAJ018094791 | ||
003 | DE-627 | ||
005 | 20230502114838.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230226s2015 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.15407/biotech8.03.045 |2 doi | |
035 | |a (DE-627)DOAJ018094791 | ||
035 | |a (DE-599)DOAJ065aed49eccd4d388ac2a366086f768f | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng |a rus |a ukr | ||
050 | 0 | |a TP248.13-248.65 | |
100 | 0 | |a А. O. Mazanova |e verfasserin |4 aut | |
245 | 1 | 0 | |a SYNTHESIS OF 25-HYDROXYVITAMIN D3 CONJUGATE WITH KEYHOLE LIMPET HEMOCYANIN AND OBTAINING OF IMMUNE SERA |
264 | 1 | |c 2015 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a The study was aimed at obtaining polyclonal antibodies that recognize 25-Hydroxyvitamin D3, for their further specifications and applications in immunochemical test systems of 25-Hydroxyvitamin D3, determination in blood serum. We used the methods of chemical synthesis of immunoconjugates (modified carbodiimide method using N´-ethyl carbodiimide), thin layer chromatography, gel filtration and indirect immunoenzyme analysis ELISA. The work describes the stages of the synthesis of 25-Hydroxyvitamin D3, immunoconjugate with keyhole limpet hemocyanin (KLH), which was used for receiving immune sera. As a result of mouse and rabbit immunization antisera were obtained and antibody titers against 25-Hydroxyvitamin D3, were tested by immunoenzyme assay. It was demonstrated that the titer of specific antibodies was higher in rabbits compared with mice. The resulting polyclonal antibodies can be used for the development of immunochemical test systems for screening studies of 25-Hydroxyvitamin D3, content in human blood serum as a marker of vitamin D3 availability. | ||
650 | 4 | |a 25-Hydroxyvitamin D3 | |
650 | 4 | |a haptens | |
650 | 4 | |a immunoenzyme analysis | |
650 | 4 | |a polyclonal antibodies | |
653 | 0 | |a Biotechnology | |
700 | 0 | |a I. O. Shymanskyy |e verfasserin |4 aut | |
700 | 0 | |a D. M. Petukhov |e verfasserin |4 aut | |
700 | 0 | |a L. B. Drobot |e verfasserin |4 aut | |
700 | 0 | |a M. M. Veliky |e verfasserin |4 aut | |
700 | 0 | |a S. V. Komisarenko |e verfasserin |4 aut | |
773 | 0 | 8 | |i In |t Biotechnologia Acta |d National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry, 2016 |g 8(2015), 3, Seite 45-55 |w (DE-627)DOAJ000108316 |x 2410776X |7 nnns |
773 | 1 | 8 | |g volume:8 |g year:2015 |g number:3 |g pages:45-55 |
856 | 4 | 0 | |u https://doi.org/10.15407/biotech8.03.045 |z kostenfrei |
856 | 4 | 0 | |u https://doaj.org/article/065aed49eccd4d388ac2a366086f768f |z kostenfrei |
856 | 4 | 0 | |u http://biotechnology.kiev.ua/images/no3_2015/mazanova_3_2015.pdf |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/2410-7751 |y Journal toc |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/2410-776X |y Journal toc |z kostenfrei |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_DOAJ | ||
912 | |a SSG-OLC-PHA | ||
951 | |a AR | ||
952 | |d 8 |j 2015 |e 3 |h 45-55 |