Comparison of Roche and Lumipulse quantitative SARS-CoV-2 antigen test performance using automated systems for the diagnosis of COVID-19
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread worldwide. Here, we evaluated the performance of two quantitative antigen (Ag) tests, the Roche and Lumipulse Ag tests, using automated platforms. Methods: We collected 637 nasopharyngeal swab samples from 274 individuals. Samples were subjected to quantitative reverse transcription PCR (RT-qPCR), the Roche Ag test and Lumipulse Ag test. Results: When RT-qPCR was used as a reference, the overall concordance rate of the Roche Ag test was 77.1% (491/637) with 70.0% (341/487) sensitivity and 100% specificity (150/150). When inconclusive results of the Lumipulse Ag test were excluded, the overall concordance rate of the Lumipulse Ag test was 88.3% (467/529) with 84.8% (330/389) sensitivity and 97.9% (137/140) specificity. The overall concordance rate between the Roche and Lumipulse Ag tests was 97.9% (518/529) with 96.7% (322/333) sensitivity and 100% (196/196) specificity. Quantitative Ag levels determined using the Roche and Lumipulse Ag tests were highly correlated (R2 = 0.922). The Roche and Lumipulse Ag tests showed high concordance up to nine days after symptom onset, with progressively lower concordance after that. Conclusions: The Roche and Lumipulse Ag tests showed equivalent assay performance and represent promising approaches for diagnosing coronavirus disease 2019..
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:108 |
---|---|
Enthalten in: |
International Journal of Infectious Diseases - 108(2021), Seite 263-269 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Yosuke Hirotsu [VerfasserIn] |
---|
Links: |
doi.org [kostenfrei] |
---|
Themen: |
Antigen |
---|
doi: |
10.1016/j.ijid.2021.05.067 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
DOAJ001519379 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | DOAJ001519379 | ||
003 | DE-627 | ||
005 | 20230309163307.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.ijid.2021.05.067 |2 doi | |
035 | |a (DE-627)DOAJ001519379 | ||
035 | |a (DE-599)DOAJb8256eedd8aa41c2b444beb2268a1aa9 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
050 | 0 | |a RC109-216 | |
100 | 0 | |a Yosuke Hirotsu |e verfasserin |4 aut | |
245 | 1 | 0 | |a Comparison of Roche and Lumipulse quantitative SARS-CoV-2 antigen test performance using automated systems for the diagnosis of COVID-19 |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread worldwide. Here, we evaluated the performance of two quantitative antigen (Ag) tests, the Roche and Lumipulse Ag tests, using automated platforms. Methods: We collected 637 nasopharyngeal swab samples from 274 individuals. Samples were subjected to quantitative reverse transcription PCR (RT-qPCR), the Roche Ag test and Lumipulse Ag test. Results: When RT-qPCR was used as a reference, the overall concordance rate of the Roche Ag test was 77.1% (491/637) with 70.0% (341/487) sensitivity and 100% specificity (150/150). When inconclusive results of the Lumipulse Ag test were excluded, the overall concordance rate of the Lumipulse Ag test was 88.3% (467/529) with 84.8% (330/389) sensitivity and 97.9% (137/140) specificity. The overall concordance rate between the Roche and Lumipulse Ag tests was 97.9% (518/529) with 96.7% (322/333) sensitivity and 100% (196/196) specificity. Quantitative Ag levels determined using the Roche and Lumipulse Ag tests were highly correlated (R2 = 0.922). The Roche and Lumipulse Ag tests showed high concordance up to nine days after symptom onset, with progressively lower concordance after that. Conclusions: The Roche and Lumipulse Ag tests showed equivalent assay performance and represent promising approaches for diagnosing coronavirus disease 2019. | ||
650 | 4 | |a SARS-CoV-2 | |
650 | 4 | |a COVID-19 | |
650 | 4 | |a Antigen | |
650 | 4 | |a Roche | |
650 | 4 | |a Lumipulse | |
653 | 0 | |a Infectious and parasitic diseases | |
700 | 0 | |a Hiroki Sugiura |e verfasserin |4 aut | |
700 | 0 | |a Makoto Maejima |e verfasserin |4 aut | |
700 | 0 | |a Miyoko Hayakawa |e verfasserin |4 aut | |
700 | 0 | |a Hitoshi Mochizuki |e verfasserin |4 aut | |
700 | 0 | |a Toshiharu Tsutsui |e verfasserin |4 aut | |
700 | 0 | |a Yumiko Kakizaki |e verfasserin |4 aut | |
700 | 0 | |a Yoshihiro Miyashita |e verfasserin |4 aut | |
700 | 0 | |a Masao Omata |e verfasserin |4 aut | |
773 | 0 | 8 | |i In |t International Journal of Infectious Diseases |d Elsevier, 2015 |g 108(2021), Seite 263-269 |w (DE-627)DOAJ000043923 |x 18783511 |7 nnns |
773 | 1 | 8 | |g volume:108 |g year:2021 |g pages:263-269 |
856 | 4 | 0 | |u https://doi.org/10.1016/j.ijid.2021.05.067 |z kostenfrei |
856 | 4 | 0 | |u https://doaj.org/article/b8256eedd8aa41c2b444beb2268a1aa9 |z kostenfrei |
856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S1201971221004707 |z kostenfrei |
856 | 4 | 2 | |u https://doaj.org/toc/1201-9712 |y Journal toc |z kostenfrei |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_DOAJ | ||
951 | |a AR | ||
952 | |d 108 |j 2021 |h 263-269 |