四妙勇安汤对ApoE~(-/-)动脉粥样硬化小鼠IL-6和MCP-1的影响 : = Effect of Simiao Yongan Decoction on IL-6 and MCP-1 in ApoE~(-/-)Atherosclerotic Mice
目的:研究四妙勇安汤对高脂饲料喂养的ApoE基因敲除小鼠动脉粥样硬化炎症反应的干预作用。方法:采用高脂饲料喂养ApoE~(-/-)小鼠14周制备动脉粥样硬化模型,成模后随机分为5组:模型组,阳性药组(阿托伐他汀,2.57 mg·kg~(-1)),四妙勇安汤高剂量组(34.9 g·kg~(-1))、四妙勇安汤中剂量组(23.14 g·kg~(-1))和四妙勇安汤低剂量组(11.61 g·kg~(-1)),分别灌胃给药12周,同时以C57BL/6小鼠为正常对照组,给予去离子水灌胃12周。采用生化仪检测三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量;HE染色观察主动脉血管形态,并测量主动脉内膜厚度(IT)、中膜厚度(MT)、血管管腔面积(LA)、粥样斑块面积(PA)、内中膜厚度比(IT/MT)、管腔狭窄率(PA/LA);采用流式高通量多因子检测技术检测血清中白介素-6(IL-6)、单核细胞趋化蛋白-1(MCP-1)炎症细胞因子表达。结果:与对照组相比,模型组小鼠血清TC、TG、LDL-C明显提高,HDL-C明显降低,PA、IT及IT/MT明显升高(P<0.01)。与模型组比较,四妙勇安汤能够明显降低ApoE~(-/-)小鼠主动脉PA、IT及IT/MT(P<0.01),并明显降低IL-6及MCP-1表达(P<0.01、P<0.05),但对血脂水平无明显的影响。结论:四妙勇安汤可以通过抑制炎症反应防治动脉粥样硬化,而不依赖血脂水平的变化。.
Objective:To study the intervention effect of Simiao Yongan decoction on atherosclerotic inflammatory response in ApoE knockout mice fed with high fat diet.Methods:ApoE~(-/-)mice were fed with high-fat diet for 14 weeks to prepare atherosclerosis model.After modeling,they were randomly divided into 5 groups:model group,positive drug group(atorvastatin,2.57 mg·kg~(-1)),Simiao Yongan decoction high dose group(34.9 g·kg~(-1)),Simiao Yongan decoction medium dose group(23.14 g·kg~(-1))and Simiao Yongan low dose group(11.61 g·kg~(-1)),respectively,administered by stomach 12 weeks.At the same time,C57 BL/6 mice were used as the normal control group,and deionized water was administered for 12 weeks.Using biochemical analyzer to detect triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(low-density lipoprotein cholesterol,LDL-C)content;HE staining to observe aortic vascular morphology,and measurement of aortic intima thickness(IT),medial thickness(MT),vascular lumen area(LA),atheroma area(PA)intima-media thickness ratio(IT/MT),lumen stenosis rate(PA/LA);interleukin-6(IL-6)and mononuclear serum,and expression of inflammatory cytokines by monocyte chemoattractant protein-1(MCP-1)were measured by flow-through high-throughput multifactor detection.Results:Compared with the control group,serum TC,TG and LDL-C in the model group were significantly increased,HDL-C was significantly decreased,and PA,IT and IT/MT were significantly increased(P<0.01).Compared with the model group,Simiao Yongan decoction significantly reduced PA,IT and IT/MT(P<0.01)in ApoE~(-/-)mice,and significantly decreased IL-6 and MCP-1 expression(P<0.01,P<0.05),but had no significant effect on blood lipid levels.Conclusion:Simiao Yongan decoction can prevent atherosclerosis by inhibiting inflammatory response,independent of changes in blood lipid levels..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019-04-15 14:02 2019 |
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| Erschienen: |
2019-04-15 14:02 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2019 |
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| Enthalten in: |
Zhong guo xian dai zhong yao - (2019), 04 vom: 15 14:02. Apr., Seite 441-447 Original Letters: Enthalten in 中国现代中药 (DE-600)2999274-6 (DE-600)2999274-6 北京市 |
| Reihe: |
China Academic Journals (CAJ), E, 医药卫生科技 = Medicine & Public Health |
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| Sprache: |
Chinesisch |
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| Weiterer Titel: |
Effect of Simiao Yongan Decoction on IL-6 and MCP-1 in ApoE~(-/-)Atherosclerotic Mice |
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| Beteiligte Personen: |
宋珂 [VerfasserIn] |
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| Links: |
oversea.cnki.net [lizenzpflichtig] |
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| Anmerkungen: |
Author info:SONG Ke;HOU Yan-hong;SU Du-na;YANG Hui;BAI Yun-qi;LI Hui;LOU Li-xia;ZHAO Yi-zhou;WU Ai-ming;TU Ya;NIE Bo;Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing,Dongzhimen Hospital,Beijing University of Chinese Medicine;Research Center for Chinese Medicine Development,China Academy of Chinese Medical Sciences;China Anding Hospital,Chifeng City,Inner Mongolia;School of Chinese Materia Medica,Beijing University of Chinese Medicine |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
CAJ630517932 |
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| 245 | 1 | 0 | |a 四妙勇安汤对ApoE~(-/-)动脉粥样硬化小鼠IL-6和MCP-1的影响 |b = Effect of Simiao Yongan Decoction on IL-6 and MCP-1 in ApoE~(-/-)Atherosclerotic Mice |
| 246 | 3 | 1 | |a Effect of Simiao Yongan Decoction on IL-6 and MCP-1 in ApoE~(-/-)Atherosclerotic Mice |
| 264 | 1 | |c 2019-04-15 14:02 | |
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| 500 | |a Author info:SONG Ke;HOU Yan-hong;SU Du-na;YANG Hui;BAI Yun-qi;LI Hui;LOU Li-xia;ZHAO Yi-zhou;WU Ai-ming;TU Ya;NIE Bo;Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing,Dongzhimen Hospital,Beijing University of Chinese Medicine;Research Center for Chinese Medicine Development,China Academy of Chinese Medical Sciences;China Anding Hospital,Chifeng City,Inner Mongolia;School of Chinese Materia Medica,Beijing University of Chinese Medicine | ||
| 520 | |a 目的:研究四妙勇安汤对高脂饲料喂养的ApoE基因敲除小鼠动脉粥样硬化炎症反应的干预作用。方法:采用高脂饲料喂养ApoE~(-/-)小鼠14周制备动脉粥样硬化模型,成模后随机分为5组:模型组,阳性药组(阿托伐他汀,2.57 mg·kg~(-1)),四妙勇安汤高剂量组(34.9 g·kg~(-1))、四妙勇安汤中剂量组(23.14 g·kg~(-1))和四妙勇安汤低剂量组(11.61 g·kg~(-1)),分别灌胃给药12周,同时以C57BL/6小鼠为正常对照组,给予去离子水灌胃12周。采用生化仪检测三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量;HE染色观察主动脉血管形态,并测量主动脉内膜厚度(IT)、中膜厚度(MT)、血管管腔面积(LA)、粥样斑块面积(PA)、内中膜厚度比(IT/MT)、管腔狭窄率(PA/LA);采用流式高通量多因子检测技术检测血清中白介素-6(IL-6)、单核细胞趋化蛋白-1(MCP-1)炎症细胞因子表达。结果:与对照组相比,模型组小鼠血清TC、TG、LDL-C明显提高,HDL-C明显降低,PA、IT及IT/MT明显升高(P<0.01)。与模型组比较,四妙勇安汤能够明显降低ApoE~(-/-)小鼠主动脉PA、IT及IT/MT(P<0.01),并明显降低IL-6及MCP-1表达(P<0.01、P<0.05),但对血脂水平无明显的影响。结论:四妙勇安汤可以通过抑制炎症反应防治动脉粥样硬化,而不依赖血脂水平的变化。 | ||
| 520 | |a Objective:To study the intervention effect of Simiao Yongan decoction on atherosclerotic inflammatory response in ApoE knockout mice fed with high fat diet.Methods:ApoE~(-/-)mice were fed with high-fat diet for 14 weeks to prepare atherosclerosis model.After modeling,they were randomly divided into 5 groups:model group,positive drug group(atorvastatin,2.57 mg·kg~(-1)),Simiao Yongan decoction high dose group(34.9 g·kg~(-1)),Simiao Yongan decoction medium dose group(23.14 g·kg~(-1))and Simiao Yongan low dose group(11.61 g·kg~(-1)),respectively,administered by stomach 12 weeks.At the same time,C57 BL/6 mice were used as the normal control group,and deionized water was administered for 12 weeks.Using biochemical analyzer to detect triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(low-density lipoprotein cholesterol,LDL-C)content;HE staining to observe aortic vascular morphology,and measurement of aortic intima thickness(IT),medial thickness(MT),vascular lumen area(LA),atheroma area(PA)intima-media thickness ratio(IT/MT),lumen stenosis rate(PA/LA);interleukin-6(IL-6)and mononuclear serum,and expression of inflammatory cytokines by monocyte chemoattractant protein-1(MCP-1)were measured by flow-through high-throughput multifactor detection.Results:Compared with the control group,serum TC,TG and LDL-C in the model group were significantly increased,HDL-C was significantly decreased,and PA,IT and IT/MT were significantly increased(P<0.01).Compared with the model group,Simiao Yongan decoction significantly reduced PA,IT and IT/MT(P<0.01)in ApoE~(-/-)mice,and significantly decreased IL-6 and MCP-1 expression(P<0.01,P<0.05),but had no significant effect on blood lipid levels.Conclusion:Simiao Yongan decoction can prevent atherosclerosis by inhibiting inflammatory response,independent of changes in blood lipid levels. | ||
| 610 | 2 | 4 | |a 北京中医药大学东直门医院中医内科学教育部和北京市重点实验室 |
| 610 | 2 | 4 | |a 中国中医科学院中医药发展研究中心 |
| 610 | 2 | 4 | |a 内蒙古赤峰市安定医院 |
| 610 | 2 | 4 | |a 北京中医药大学中药学院 |
| 650 | 4 | |a 中药学、方剂学 | |
| 650 | 4 | |a 中医 | |
| 650 | 4 | |a 医药、卫生 | |
| 650 | 4 | |a Traditional Chinese Medicinal Herbs | |
| 650 | 4 | |a 医药卫生科技 | |
| 650 | 4 | |a Medicine & Public Health | |
| 650 | 4 | |a 四妙勇安汤 | |
| 650 | 4 | |a 动脉粥样硬化 | |
| 650 | 4 | |a ApoE~(-/-)小鼠 | |
| 650 | 4 | |a 病理 | |
| 650 | 4 | |a 白介素-6 | |
| 650 | 4 | |a 单核细胞趋化蛋白-1 | |
| 650 | 4 | |a Simiao Yongan decoction | |
| 650 | 4 | |a atherosclerosis | |
| 650 | 4 | |a ApoE~(-/-)mice | |
| 650 | 4 | |a pathology | |
| 650 | 4 | |a IL-6 | |
| 650 | 4 | |a MCP-1 | |
| 700 | 0 | |a 侯彦宏 |4 oth | |
| 700 | 0 | |a 苏都娜 |4 oth | |
| 700 | 0 | |a 杨会 |4 oth | |
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| 700 | 0 | |a 李慧 |4 oth | |
| 700 | 0 | |a 娄利霞 |4 oth | |
| 700 | 0 | |a 赵一舟 |4 oth | |
| 700 | 0 | |a 吴爱明 |4 oth | |
| 700 | 0 | |a 图雅 |4 oth | |
| 700 | 0 | |a 聂波 |4 oth | |
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