足底电击法诱导大鼠内脏高敏感模型的建立和评价 : = Establishment and evaluation of a rat model of footshock-induced visceral hypersensitivity
目的通过足底电击建立大鼠内脏高敏感动物模型,并评价其有效性和优势。方法将雌性SD大鼠随机分为正常对照(NC)组、足底电击(FSS)组和避水应激(WAS)组。NC组每天放入模拟应激箱中1 h不予刺激;FSS组每天置于电击箱中1 h,给予电击刺激,刺激电压为40 V,刺激频率为20次/min,每次1 s,共5 min;WAS组每天给予1 h避水应激;造模期为10 d。观察记录大鼠一般情况和排便情况,用腹壁回缩反射(AWR)检测内脏敏感性,用ELISA法检测血清CRF、ACTH、CORT、5-HT浓度和结肠组织5-HT浓度。结果 (1) FSS组大鼠的内脏敏感性明显高于WAS组和NC组(P<0.05),结肠黏膜无明显病理性损伤。(2) FSS组大鼠血清CRF、ACTH、CORT、5-HT浓度和结肠组织5-HT浓度均较WAS组和NC组明显升高(P<0.05)。(3) FSS组大鼠排便量明显高于WAS组和NC组(P<0.05),其粪便含水量明显高于NC组(P<0.05),而且其大鼠排便量和粪便含水量数值稳定。结论足底电击造模法可建立大鼠内脏高敏感模型且优于避水应激法。.
Objective To establish and evaluate a new rat model of visceral hypersensitivity(VH) induced by electric footshock. Methods Female SD rats were divided randomly into the normal control(NC) group, footshock stress(FSS) group, and water avoidance stress(WAS) group. The NC rats were placed in a footshock stress box for 1 h every day without stimulation. The FSS rats were placed in a footshock stress box for 1 h every day and stimulated by electric shock(U=40 v, t=1 s, f=20 times/min) for 5 min, and WAS rats were placed in a WAS box for 1 h every day. All groups were treated for consecutive 10 days. The general status and defecation status of the rats were recorded every other day. Visceral sensitivity was evaluated with abdominal withdrawal reflex(AWR) test. The serum concentrations of CRF, ACTH, CORT, and 5-HT and the colonic concentration of 5-HT were measured by ELISA. Results 1) Compared with the WAS and NC rats, the visceral sensitivity of FSS rats was significantly increased(P<0.05). There were no significant pathological manifestations in the colons of FSS or WAS rats.(2) Compared with the WAS and NC rats, the serum concentrations of CRF, ACTH, CORT, and 5-HT and the colonic concentration of 5-HT were all significantly increased in the FSS rats(P<0.05).(3) The defecation quantity of FSS rats was significantly greater than those of WAS and NC rats(P<0.05). The water content of the feces of FSS rats was significantly higher than that of NC rats(P<0.05). The defecation quantity and water content in the feces of FSS rats were stable during the experiment. Conclusions FSS is an optimal method for establishing a rat model of VH and is superior to water avoidance stress..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018-12-29 14:15 2019 |
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| Erschienen: |
2018-12-29 14:15 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2019 |
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| Enthalten in: |
Zhong guo shi yan dong wu xue bao - (2019), 01 vom: 29 14:15. Dez., Seite 46-51 Original Letters: Enthalten in 中国实验动物学报 (DE-600)2990019-0 (DE-600)2990019-0 北京市 |
| Reihe: |
China Academic Journals (CAJ), A, 理工A(数学物理力学天地生) = Mathematics/ Physics/ Mechanics/ Astronomy China Academic Journals (CAJ), E, 医药卫生科技 = Medicine & Public Health |
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| Sprache: |
Chinesisch |
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| Weiterer Titel: |
Establishment and evaluation of a rat model of footshock-induced visceral hypersensitivity |
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| Beteiligte Personen: |
赵磊 [VerfasserIn] |
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| Links: |
oversea.cnki.net [lizenzpflichtig] |
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| Anmerkungen: |
Author info:ZHAO Lei;LI Xi;YUAN Jianye;LIN Jiang;Shanghai University of Traditional Chinese Medicine;Department of Gastroenterology,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
CAJ624175782 |
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| 245 | 1 | 0 | |a 足底电击法诱导大鼠内脏高敏感模型的建立和评价 |b = Establishment and evaluation of a rat model of footshock-induced visceral hypersensitivity |
| 246 | 3 | 1 | |a Establishment and evaluation of a rat model of footshock-induced visceral hypersensitivity |
| 264 | 1 | |c 2018-12-29 14:15 | |
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| 490 | 0 | |a China Academic Journals (CAJ) |a E |a 医药卫生科技 = Medicine & Public Health | |
| 500 | |a Author info:ZHAO Lei;LI Xi;YUAN Jianye;LIN Jiang;Shanghai University of Traditional Chinese Medicine;Department of Gastroenterology,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine | ||
| 520 | |a 目的通过足底电击建立大鼠内脏高敏感动物模型,并评价其有效性和优势。方法将雌性SD大鼠随机分为正常对照(NC)组、足底电击(FSS)组和避水应激(WAS)组。NC组每天放入模拟应激箱中1 h不予刺激;FSS组每天置于电击箱中1 h,给予电击刺激,刺激电压为40 V,刺激频率为20次/min,每次1 s,共5 min;WAS组每天给予1 h避水应激;造模期为10 d。观察记录大鼠一般情况和排便情况,用腹壁回缩反射(AWR)检测内脏敏感性,用ELISA法检测血清CRF、ACTH、CORT、5-HT浓度和结肠组织5-HT浓度。结果 (1) FSS组大鼠的内脏敏感性明显高于WAS组和NC组(P<0.05),结肠黏膜无明显病理性损伤。(2) FSS组大鼠血清CRF、ACTH、CORT、5-HT浓度和结肠组织5-HT浓度均较WAS组和NC组明显升高(P<0.05)。(3) FSS组大鼠排便量明显高于WAS组和NC组(P<0.05),其粪便含水量明显高于NC组(P<0.05),而且其大鼠排便量和粪便含水量数值稳定。结论足底电击造模法可建立大鼠内脏高敏感模型且优于避水应激法。 | ||
| 520 | |a Objective To establish and evaluate a new rat model of visceral hypersensitivity(VH) induced by electric footshock. Methods Female SD rats were divided randomly into the normal control(NC) group, footshock stress(FSS) group, and water avoidance stress(WAS) group. The NC rats were placed in a footshock stress box for 1 h every day without stimulation. The FSS rats were placed in a footshock stress box for 1 h every day and stimulated by electric shock(U=40 v, t=1 s, f=20 times/min) for 5 min, and WAS rats were placed in a WAS box for 1 h every day. All groups were treated for consecutive 10 days. The general status and defecation status of the rats were recorded every other day. Visceral sensitivity was evaluated with abdominal withdrawal reflex(AWR) test. The serum concentrations of CRF, ACTH, CORT, and 5-HT and the colonic concentration of 5-HT were measured by ELISA. Results 1) Compared with the WAS and NC rats, the visceral sensitivity of FSS rats was significantly increased(P<0.05). There were no significant pathological manifestations in the colons of FSS or WAS rats.(2) Compared with the WAS and NC rats, the serum concentrations of CRF, ACTH, CORT, and 5-HT and the colonic concentration of 5-HT were all significantly increased in the FSS rats(P<0.05).(3) The defecation quantity of FSS rats was significantly greater than those of WAS and NC rats(P<0.05). The water content of the feces of FSS rats was significantly higher than that of NC rats(P<0.05). The defecation quantity and water content in the feces of FSS rats were stable during the experiment. Conclusions FSS is an optimal method for establishing a rat model of VH and is superior to water avoidance stress. | ||
| 610 | 2 | 4 | |a 上海中医药大学 |
| 610 | 2 | 4 | |a 上海中医药大学附属龙华医院消化科 |
| 610 | 2 | 4 | |a 上海中医药大学脾胃病研究所 |
| 650 | 4 | |a 医药、卫生 | |
| 650 | 4 | |a 消化系及腹部疾病 | |
| 650 | 4 | |a 内科学 | |
| 650 | 4 | |a Fundamental Medicine | |
| 650 | 4 | |a Digestive System Disease | |
| 650 | 4 | |a 理工A(数学物理力学天地生) | |
| 650 | 4 | |a Mathematics/ Physics/ Mechanics/ Astronomy | |
| 650 | 4 | |a 医药卫生科技 | |
| 650 | 4 | |a Medicine & Public Health | |
| 650 | 4 | |a 内脏高敏感 | |
| 650 | 4 | |a 足底电击 | |
| 650 | 4 | |a 动物模型 | |
| 650 | 4 | |a visceral hypersensitivity | |
| 650 | 4 | |a footshock | |
| 650 | 4 | |a animal model | |
| 650 | 4 | |a rat | |
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| 700 | 0 | |a 袁建业 |4 oth | |
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