清热解毒方体外抗人乳头瘤病毒感染及作用机制 : = Effect of Qingre Jiedu Prescription in Resisting Human Papllimavirus Infection in Vitro and Its Mechanism
目的:采用高危型人乳头瘤状病毒16(human papillomavirus 16,HPV16)体外假病毒感染模型评价清热解毒方体外抗HPV16感染活性及作用方式,并探讨清热解毒方的作用机制。方法:首先,将HPV16假病毒加入到293T/17细胞中,建立HPV假病毒体外荧光感染模型,以此模型评价清热解毒方(20~1 000 g·L~(-1))体外抗HPV16作用,探讨清热解毒方抗HPV感染的量效关系;其次,采用HPV假病毒模型,通过预处理细胞、与病毒同时作用于细胞、病毒处理后作用于细胞的不同处理方法,探讨清热解毒方(20 g·L~(-1))抗HPV的作用方式;最后,从清热解毒方作用后HPV转化的He La细胞中提取致癌蛋白E7,细胞外信号调节激酶(ERK)以及核转录因子-κB(NF-κB),用蛋白免疫印迹法(Western blot)检测E7,ERK,NF-κB蛋白表达,探讨清热解毒方(3. 33~20 g·L~(-1))在肿瘤相关蛋白水平上的作用机制。结果:与病毒组比较,清热解毒方可以明显抑制HPV16假病毒的感染(P <0. 05),且呈现明显的浓度依赖关系;且清热解毒方能够通过保护细胞、抑制病毒吸附并抑制病毒感染后过程的多种方式发挥其抗HPV16作用,其中保护细胞的效果更显著(P <0. 01),说明清热解毒方能够显著预防HPV16感染;与空白组比较,清热解毒方能够明显抑制E7致癌蛋白的表达,并抑制ERK,NF-κB蛋白的表达(P <0. 05,P <0. 01),达到抗肿瘤的作用。结论:清热解毒方具有较好的抑制HPV病毒的效果,该研究为清热解毒方清除HPV病毒和防治宫颈癌提供了创新性的理论依据。.
Objective: To assess the activity and action modes of Qingre Jiedu prescription in resisting human papillomavirus 16(HPV16) in vitro by establishing the cell model infected by HPV16 pseudovirus particles,in order to explore the mechanism of action of Qingre Jiedu prescription on transformed HeLa cell HPV.Method: Firstly,HPV16 pseudovirus particles were added to 293 T/17 cells to establish an HPV pseudovirus infection model,so as to detect fluorescence intensity in vitro. Then this model was used to evaluate the effect of Qingre Jiedu prescription in resisting HPV16 infection in vitro and its dose-effect relationship of Qingre Jiedu prescription(20 ~ 1 000 g·L~(-1)). Secondly,the HPV pseudovirus infection model was used to explore the action modes of Qingre Jiedu prescription(20 g·L~(-1)) against HPV under such different conditions as pre-treating the cells,acting the cells simultaneously with virus and acting the cells after virus treatment. Finally,the mechanism of Qingre Jiedu prescription(3. 33 ~ 20 g·L~(-1)) was explored by Western blot. In brief,the oncogenic protein E7,extracellular signal-regulated kinase(ERK) and nuclear factor-κB(NF-κB) were extracted from HeLa cells HPV transformed after the action of Qingre Jiedu prescription and detected by Western blot. These proteins were compared with the corresponding protein expression levels in the blank group. Result: The results of experiment on HPV16 pseudovirus infection model showed that Qingre Jiedu prescription could significantly inhibit the infection of HPV16 pseudovirus in cells(P < 0. 05),in an obvious concentration-dependent manner. The action modes of Qingre Jiedu prescription in the HPV16 pseudovirus infection model in vitro showed that Qingre Jiedu prescription could protect the cells,inhibit the absorption and post-virus infection process. And the drug can significantly prevent HPV16 infection because it could protect cells from the HPV16 infection(P < 0. 01). These results show that Qingre Jiedu prescription could protect cells from the HPV16 infection,inhibit the absorption and post-virus infection process. In addition,Qingre Jiedu prescription could obviously inhibit the expressions of oncoprotein E7,and ERK and NF-κB protein,so as to achieve the antitumor effect(P < 0. 05,P < 0. 01). Conclusion: Qingre Jiedu prescription has a better effect in inhibiting HPV virus,and the HPV16 pseudovirus infection experiment provides the innovative theory basis for the application of Qingre Jiedu prescription in anti-HPV and cervical cancer therapy..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018-11-20 2018 |
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| Erschienen: |
2018-11-20 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2018 |
|---|---|
| Enthalten in: |
Zhong guo shi yan fang ji xue za zhi - (2018), 24 vom: 20. Nov., Seite 171-176 Original Letters: Enthalten in 中国实验方剂学杂志 (DE-600)2990049-9 (DE-600)2990049-9 北京市 |
| Reihe: |
China Academic Journals (CAJ), E, 医药卫生科技 = Medicine & Public Health |
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| Sprache: |
Chinesisch |
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| Weiterer Titel: |
Effect of Qingre Jiedu Prescription in Resisting Human Papllimavirus Infection in Vitro and Its Mechanism |
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| Beteiligte Personen: |
冉雪梦 [VerfasserIn] |
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| Links: |
oversea.cnki.net [lizenzpflichtig] |
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| Anmerkungen: |
Author info:RAN Xue-meng;WANG Shi-xing;ZHANG Li-yan;LIU Xiao-feng;SUN Xuan;Qingdao Hiser Medical Center;School of Medicine and Pharmacy,Ocean University of China |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
CAJ610285475 |
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| 245 | 1 | 0 | |a 清热解毒方体外抗人乳头瘤病毒感染及作用机制 |b = Effect of Qingre Jiedu Prescription in Resisting Human Papllimavirus Infection in Vitro and Its Mechanism |
| 246 | 3 | 1 | |a Effect of Qingre Jiedu Prescription in Resisting Human Papllimavirus Infection in Vitro and Its Mechanism |
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| 500 | |a Author info:RAN Xue-meng;WANG Shi-xing;ZHANG Li-yan;LIU Xiao-feng;SUN Xuan;Qingdao Hiser Medical Center;School of Medicine and Pharmacy,Ocean University of China | ||
| 520 | |a 目的:采用高危型人乳头瘤状病毒16(human papillomavirus 16,HPV16)体外假病毒感染模型评价清热解毒方体外抗HPV16感染活性及作用方式,并探讨清热解毒方的作用机制。方法:首先,将HPV16假病毒加入到293T/17细胞中,建立HPV假病毒体外荧光感染模型,以此模型评价清热解毒方(20~1 000 g·L~(-1))体外抗HPV16作用,探讨清热解毒方抗HPV感染的量效关系;其次,采用HPV假病毒模型,通过预处理细胞、与病毒同时作用于细胞、病毒处理后作用于细胞的不同处理方法,探讨清热解毒方(20 g·L~(-1))抗HPV的作用方式;最后,从清热解毒方作用后HPV转化的He La细胞中提取致癌蛋白E7,细胞外信号调节激酶(ERK)以及核转录因子-κB(NF-κB),用蛋白免疫印迹法(Western blot)检测E7,ERK,NF-κB蛋白表达,探讨清热解毒方(3. 33~20 g·L~(-1))在肿瘤相关蛋白水平上的作用机制。结果:与病毒组比较,清热解毒方可以明显抑制HPV16假病毒的感染(P <0. 05),且呈现明显的浓度依赖关系;且清热解毒方能够通过保护细胞、抑制病毒吸附并抑制病毒感染后过程的多种方式发挥其抗HPV16作用,其中保护细胞的效果更显著(P <0. 01),说明清热解毒方能够显著预防HPV16感染;与空白组比较,清热解毒方能够明显抑制E7致癌蛋白的表达,并抑制ERK,NF-κB蛋白的表达(P <0. 05,P <0. 01),达到抗肿瘤的作用。结论:清热解毒方具有较好的抑制HPV病毒的效果,该研究为清热解毒方清除HPV病毒和防治宫颈癌提供了创新性的理论依据。 | ||
| 520 | |a Objective: To assess the activity and action modes of Qingre Jiedu prescription in resisting human papillomavirus 16(HPV16) in vitro by establishing the cell model infected by HPV16 pseudovirus particles,in order to explore the mechanism of action of Qingre Jiedu prescription on transformed HeLa cell HPV.Method: Firstly,HPV16 pseudovirus particles were added to 293 T/17 cells to establish an HPV pseudovirus infection model,so as to detect fluorescence intensity in vitro. Then this model was used to evaluate the effect of Qingre Jiedu prescription in resisting HPV16 infection in vitro and its dose-effect relationship of Qingre Jiedu prescription(20 ~ 1 000 g·L~(-1)). Secondly,the HPV pseudovirus infection model was used to explore the action modes of Qingre Jiedu prescription(20 g·L~(-1)) against HPV under such different conditions as pre-treating the cells,acting the cells simultaneously with virus and acting the cells after virus treatment. Finally,the mechanism of Qingre Jiedu prescription(3. 33 ~ 20 g·L~(-1)) was explored by Western blot. In brief,the oncogenic protein E7,extracellular signal-regulated kinase(ERK) and nuclear factor-κB(NF-κB) were extracted from HeLa cells HPV transformed after the action of Qingre Jiedu prescription and detected by Western blot. These proteins were compared with the corresponding protein expression levels in the blank group. Result: The results of experiment on HPV16 pseudovirus infection model showed that Qingre Jiedu prescription could significantly inhibit the infection of HPV16 pseudovirus in cells(P < 0. 05),in an obvious concentration-dependent manner. The action modes of Qingre Jiedu prescription in the HPV16 pseudovirus infection model in vitro showed that Qingre Jiedu prescription could protect the cells,inhibit the absorption and post-virus infection process. And the drug can significantly prevent HPV16 infection because it could protect cells from the HPV16 infection(P < 0. 01). These results show that Qingre Jiedu prescription could protect cells from the HPV16 infection,inhibit the absorption and post-virus infection process. In addition,Qingre Jiedu prescription could obviously inhibit the expressions of oncoprotein E7,and ERK and NF-κB protein,so as to achieve the antitumor effect(P < 0. 05,P < 0. 01). Conclusion: Qingre Jiedu prescription has a better effect in inhibiting HPV virus,and the HPV16 pseudovirus infection experiment provides the innovative theory basis for the application of Qingre Jiedu prescription in anti-HPV and cervical cancer therapy. | ||
| 610 | 2 | 4 | |a 青岛市海慈医疗集团 |
| 610 | 2 | 4 | |a 中国海洋大学医药学院 |
| 650 | 4 | |a 中药学、方剂学 | |
| 650 | 4 | |a 中医 | |
| 650 | 4 | |a 医药、卫生 | |
| 650 | 4 | |a Traditional Chinese Medicinal Herbs | |
| 650 | 4 | |a 医药卫生科技 | |
| 650 | 4 | |a Medicine & Public Health | |
| 650 | 4 | |a 清热解毒方 | |
| 650 | 4 | |a 人乳头瘤病毒 | |
| 650 | 4 | |a 假病毒模型 | |
| 650 | 4 | |a 作用机制 | |
| 650 | 4 | |a Qingre Jiedu prescription | |
| 650 | 4 | |a human papillomavirus(HPV) | |
| 650 | 4 | |a pseudovirus model | |
| 650 | 4 | |a mechanism of action | |
| 700 | 0 | |a 王世欣 |4 oth | |
| 700 | 0 | |a 张立岩 |4 oth | |
| 700 | 0 | |a 刘啸风 |4 oth | |
| 700 | 0 | |a 孙选 |4 oth | |
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