Structural and functional characterization of a novel ShK peptide and its analogue from zoanthids(corals) : = Structural and functional characterization of a novel ShK peptide and its analogue from zoanthids(corals)
OBJECTIVE To identify and characterize a novel neuroprotective ShK peptide and its analogue originated from coral P.caribaeoru.METHODS P.caribaeoru was collected and subjected to transcriptome sequencing at which further bioinformatics analysis had identified a unigene encoding a putative ShK protein candidate,named as PcShK1.PcShK1 and its rhodamine derivative(PcShK1-RhoB) were synthesized and tested for the neuroprotective effect in 6-OHDA induced Parkinson disease models in vitro and in vivo.Briefly,zebrafish larvae were co-exposed to 6-OHDA and various doses of the peptides;then,dopaminergic(DA) neurons immunoreactivity and locomotion behavior of the zebrafish larvae were examined.Similarly,PC12 cells were cultured with 6-OHDA in the absence or presence of different concentrations of peptides.Cell viability was determined by MTT assay while calcium flow in the PC12 cells was monitored by Fluo-4 fluorescent dye.RESULTS Compared with control group,6-OHDA treatment could lead to DA neurons loss and locomotion deficits in PD model of zebrafish larvae(P<0.01).Both PcS hK 1(2.5,5.0 and 7.5 μmol·L~(-1)) and PcS hK 1-RhoB(0.50 and 0.75 μmol·L~(-1)) were found to protect and restore dopaminergic neurons from6-OHDA mediated injury and locomotion deficiency in the PD zebrafish respectively(P<0.01).In addition,PcShK1(2.5 to 20.0 μmol·L~(-1)) and PcShK1-RhoB(0.6 to 2.5 μmol·L~(-1)) effectively prevented against 6-OHDA toxicity in PC12 cells(P<0.01).Further study revealed that they might exert their neuroprotective effects through regulating the calcium homeostasis.CONCLUSION PcS hK 1 and PcS hK 1-RhoB show neuroprotective effects on 6-OHDA induced PD models,and the underlying protective mechanisms of these peptides probably involve calcium homeostasis regulation..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018-09-15 2018 |
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| Erschienen: |
2018-09-15 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2018 |
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| Enthalten in: |
Zhong guo yao li xue yu du li xue za zhi - (2018), 09 vom: 15. Sept., Seite 708-709 Original Letters: Enthalten in 中国药理学与毒理学杂志 (DE-600)2989809-2 (DE-600)2989809-2 北京市 |
| Reihe: |
China Academic Journals (CAJ), E, 医药卫生科技 = Medicine & Public Health |
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| Sprache: |
Chinesisch |
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| Weiterer Titel: |
Structural and functional characterization of a novel ShK peptide and its analogue from zoanthids(corals) |
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| Beteiligte Personen: |
GONG Gui-yi [VerfasserIn] |
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| Links: |
oversea.cnki.net [lizenzpflichtig] |
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| Anmerkungen: |
Author info:GONG Gui-yi;LIAO Qi-wen;Yu-chung TSE;Simon Ming-yuen LEE;University of Macau;South University of Science and Technology of China |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
CAJ608037796 |
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| 245 | 1 | 0 | |a Structural and functional characterization of a novel ShK peptide and its analogue from zoanthids(corals) |b = Structural and functional characterization of a novel ShK peptide and its analogue from zoanthids(corals) |
| 246 | 3 | 1 | |a Structural and functional characterization of a novel ShK peptide and its analogue from zoanthids(corals) |
| 264 | 1 | |c 2018-09-15 | |
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| 500 | |a Author info:GONG Gui-yi;LIAO Qi-wen;Yu-chung TSE;Simon Ming-yuen LEE;University of Macau;South University of Science and Technology of China | ||
| 520 | |a OBJECTIVE To identify and characterize a novel neuroprotective ShK peptide and its analogue originated from coral P.caribaeoru.METHODS P.caribaeoru was collected and subjected to transcriptome sequencing at which further bioinformatics analysis had identified a unigene encoding a putative ShK protein candidate,named as PcShK1.PcShK1 and its rhodamine derivative(PcShK1-RhoB) were synthesized and tested for the neuroprotective effect in 6-OHDA induced Parkinson disease models in vitro and in vivo.Briefly,zebrafish larvae were co-exposed to 6-OHDA and various doses of the peptides;then,dopaminergic(DA) neurons immunoreactivity and locomotion behavior of the zebrafish larvae were examined.Similarly,PC12 cells were cultured with 6-OHDA in the absence or presence of different concentrations of peptides.Cell viability was determined by MTT assay while calcium flow in the PC12 cells was monitored by Fluo-4 fluorescent dye.RESULTS Compared with control group,6-OHDA treatment could lead to DA neurons loss and locomotion deficits in PD model of zebrafish larvae(P<0.01).Both PcS hK 1(2.5,5.0 and 7.5 μmol·L~(-1)) and PcS hK 1-RhoB(0.50 and 0.75 μmol·L~(-1)) were found to protect and restore dopaminergic neurons from6-OHDA mediated injury and locomotion deficiency in the PD zebrafish respectively(P<0.01).In addition,PcShK1(2.5 to 20.0 μmol·L~(-1)) and PcShK1-RhoB(0.6 to 2.5 μmol·L~(-1)) effectively prevented against 6-OHDA toxicity in PC12 cells(P<0.01).Further study revealed that they might exert their neuroprotective effects through regulating the calcium homeostasis.CONCLUSION PcS hK 1 and PcS hK 1-RhoB show neuroprotective effects on 6-OHDA induced PD models,and the underlying protective mechanisms of these peptides probably involve calcium homeostasis regulation. | ||
| 610 | 2 | 4 | |a University of Macau |
| 610 | 2 | 4 | |a South University of Science and Technology of China |
| 650 | 4 | |a 药理学 | |
| 650 | 4 | |a 药学 | |
| 650 | 4 | |a 医药、卫生 | |
| 650 | 4 | |a Pharmaceutics | |
| 650 | 4 | |a 医药卫生科技 | |
| 650 | 4 | |a Medicine & Public Health | |
| 650 | 4 | |a transcriptome | |
| 650 | 4 | |a coral P.caribaeoru | |
| 650 | 4 | |a neuroprotective | |
| 650 | 4 | |a zebrafish | |
| 650 | 4 | |a calcium homeostasis | |
| 700 | 0 | |a LIAO Qi-wen |4 oth | |
| 700 | 0 | |a Yu-chung TSE |4 oth | |
| 700 | 0 | |a Simon Ming-yuen LEE |4 oth | |
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