Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells : = Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells
AIM To evaluate the influence of hyperglycemia on the progression of autoimmune pancreatitis.METHODS We induced hyperglycemia by repetitive intraperitoneal(ip) injection of 50 mg/kg streptozotocin in MRL/Mp J mice, which develop autoimmune pancreatitis due to a genetic predisposition. We compared the extent of inflammation(histological score, CD3~+ lymphocytes, CD8~+ T-cells, CD4~+ T-cells, Foxp3~+ T-helper cells) in the pancreas of hyperglycemic and normoglycemic mice. We also analyzed the number of leukocytes, lymphocytes, granulocytes and monocytes in the blood. In addition, we determined the percentage of CD3~+ lymphocytes, CD8~+ T-cells, CD4~+ T-cells, Foxp3~+ T-helper cells, Foxp3~+ CD25~+ T-helper and Foxp3~+T-helper cells in the spleen by flow cytometry.RESULTS Treatment with streptozotocin caused a strong induction of hyperglycemia and a reduction in body weight(P < 0.001). Severe hyperglycemia did not, however, lead to an aggravation, but rather to a slight attenuation of autoimmune pancreatitis. In the pancreas, both the histological score of the pancreas as well as the number of CD3+ lymphocytes(P < 0.053) were decreased by hyperglycemia. No major changes in the percentage of CD8~+ T-cells, CD4~+ T-cells, Foxp3~+ T-helper cells were observed between hyperglycemic and normoglycemic mice. Hyperglycemia increased the numbers of leukocytes(P < 0.001), lymphocytes(P = 0.016), granulocytes and monocytes(P = 0.001) in the blood. Hyperglycemia also moderately reduced the percentage of CD3~+ lymphocytes(P = 0.057), significantly increased the percentage of Foxp3~+ T-helper cells(P = 0.018) and Foxp3~+ CD25~+ T-helper cells(P = 0.021) and reduced the percentage of Foxp3~+T-helper cells(P = 0.034) in the spleen. CONCLUSION Hyperglycemia does not aggravate but moderately attenuates autoimmune pancreatitis, possibly by increasing the percentage of regulatory T-cells in the spleen..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2018-07-28 2018 |
|---|---|
| Erschienen: |
2018-07-28 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2018 |
|---|---|
| Enthalten in: |
World journal of gastroenterology - (2018), 28 vom: 28. Juli, Seite 3120-3129 Original Letters: Enthalten in (DE-576)276271955 (DE-576)276271955 |
| Reihe: |
China Academic Journals (CAJ), E, 医药卫生科技 = Medicine & Public Health |
|---|
| Sprache: |
Chinesisch |
|---|
| Weiterer Titel: |
Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells |
|---|
| Beteiligte Personen: |
Franz-Tassilo Müller-Graff [VerfasserIn] |
|---|
| Links: |
oversea.cnki.net [lizenzpflichtig] |
|---|
| Anmerkungen: |
Author info:Franz-Tassilo Müller-Graff;Brit Fitzner;Robert Jaster;Brigitte Vollmar;Dietmar Zechner;Institute for Experimental Surgery, Rostock University Medical Center;Division of Gastroenterology,Department of Medicine Ⅱ, Rostock University Medical Center |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
CAJ603241263 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | CAJ603241263 | ||
| 003 | DE-627 | ||
| 005 | 20230318132326.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221230s2018 cc |||||o 00| ||chi c | ||
| 035 | |a (DE-627)CAJ603241263 | ||
| 035 | |a (SBB-XA)CAJ_ZXXY201828006 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a chi | ||
| 044 | |c XB-CN | ||
| 084 | |a ASIEN |q DE-1a |2 fid | ||
| 084 | |a R576 |2 clc | ||
| 100 | 0 | |a Franz-Tassilo Müller-Graff |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells |b = Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells |
| 246 | 3 | 1 | |a Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells |
| 264 | 1 | |c 2018-07-28 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 490 | 0 | |a China Academic Journals (CAJ) |a E |a 医药卫生科技 = Medicine & Public Health | |
| 500 | |a Author info:Franz-Tassilo Müller-Graff;Brit Fitzner;Robert Jaster;Brigitte Vollmar;Dietmar Zechner;Institute for Experimental Surgery, Rostock University Medical Center;Division of Gastroenterology,Department of Medicine Ⅱ, Rostock University Medical Center | ||
| 520 | |a AIM To evaluate the influence of hyperglycemia on the progression of autoimmune pancreatitis.METHODS We induced hyperglycemia by repetitive intraperitoneal(ip) injection of 50 mg/kg streptozotocin in MRL/Mp J mice, which develop autoimmune pancreatitis due to a genetic predisposition. We compared the extent of inflammation(histological score, CD3~+ lymphocytes, CD8~+ T-cells, CD4~+ T-cells, Foxp3~+ T-helper cells) in the pancreas of hyperglycemic and normoglycemic mice. We also analyzed the number of leukocytes, lymphocytes, granulocytes and monocytes in the blood. In addition, we determined the percentage of CD3~+ lymphocytes, CD8~+ T-cells, CD4~+ T-cells, Foxp3~+ T-helper cells, Foxp3~+ CD25~+ T-helper and Foxp3~+T-helper cells in the spleen by flow cytometry.RESULTS Treatment with streptozotocin caused a strong induction of hyperglycemia and a reduction in body weight(P < 0.001). Severe hyperglycemia did not, however, lead to an aggravation, but rather to a slight attenuation of autoimmune pancreatitis. In the pancreas, both the histological score of the pancreas as well as the number of CD3+ lymphocytes(P < 0.053) were decreased by hyperglycemia. No major changes in the percentage of CD8~+ T-cells, CD4~+ T-cells, Foxp3~+ T-helper cells were observed between hyperglycemic and normoglycemic mice. Hyperglycemia increased the numbers of leukocytes(P < 0.001), lymphocytes(P = 0.016), granulocytes and monocytes(P = 0.001) in the blood. Hyperglycemia also moderately reduced the percentage of CD3~+ lymphocytes(P = 0.057), significantly increased the percentage of Foxp3~+ T-helper cells(P = 0.018) and Foxp3~+ CD25~+ T-helper cells(P = 0.021) and reduced the percentage of Foxp3~+T-helper cells(P = 0.034) in the spleen. CONCLUSION Hyperglycemia does not aggravate but moderately attenuates autoimmune pancreatitis, possibly by increasing the percentage of regulatory T-cells in the spleen. | ||
| 610 | 2 | 4 | |a Institute for Experimental Surgery, Rostock University Medical Center |
| 610 | 2 | 4 | |a Division of Gastroenterology,Department of Medicine Ⅱ, Rostock University Medical Center |
| 650 | 4 | |a 消化系及腹部疾病 | |
| 650 | 4 | |a 内科学 | |
| 650 | 4 | |a 医药、卫生 | |
| 650 | 4 | |a Digestive System Disease | |
| 650 | 4 | |a 医药卫生科技 | |
| 650 | 4 | |a Medicine & Public Health | |
| 650 | 4 | |a Autoimmune disease | |
| 650 | 4 | |a Diabetes | |
| 650 | 4 | |a Treg | |
| 650 | 4 | |a FoxP3 | |
| 650 | 4 | |a Autoimmune pancreatitis | |
| 650 | 4 | |a MRL/MpJ mice | |
| 700 | 0 | |a Brit Fitzner |4 oth | |
| 700 | 0 | |a Robert Jaster |4 oth | |
| 700 | 0 | |a Brigitte Vollmar |4 oth | |
| 700 | 0 | |a Dietmar Zechner |4 oth | |
| 773 | 0 | 8 | |6 880-01 |i Enthalten in |t World journal of gastroenterology |d Beijing : WJG Press, 1995 |g (2018), 28 vom: 28. Juli, Seite 3120-3129 |h Online-Ressource |w (DE-627)CAJ318592460 |w (DE-600)2084831-6 |w (DE-576)276271955 |x 2219-2840 |7 nnns |
| 773 | 1 | 8 | |g year:2018 |g number:28 |g day:28 |g month:07 |g pages:3120-3129 |
| 856 | 4 | 0 | |u http://erf.sbb.spk-berlin.de/han/caj/oversea.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&filename=ZXXY201828006 |x Verlag |y CrossAsia Link |z Deutschlandweit zugänglich |
| 856 | 4 | 0 | |u https://oversea.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&filename=ZXXY201828006 |x Verlag |z lizenzpflichtig |
| 880 | 0 | 8 | |6 773-01/Hans |i Enthalten in |h Online-Ressource |w (DE-627)CAJ318592460 |w (DE-600)2084831-6 |w (DE-576)276271955 |x 2219-2840 |7 nnns |
| 912 | |a ZDB-1-CAJ | ||
| 912 | |a GBV_NL_CAJ | ||
| 912 | |a FID-ASIEN | ||
| 912 | |a SSG-OLC-OA3.1 | ||
| 912 | |a SSG-OLC-CAJ | ||
| 936 | u | w | |j 2018 |e 28 |b 28 |c 07 |h 3120-3129 |
| 951 | |a AR | ||
| 952 | |j 2018 |e 28 |b 28 |c 07 |h 3120-3129 | ||
| 980 | |2 11 |1 01 |x 0001 |b 622742817 |c 00 |f 5:FIDXASIA |d --%%-- |e --%%-- |j --%%-- |y k |z 30-12-22 | ||
| 981 | |2 11 |1 01 |x 0001 |r http://erf.sbb.spk-berlin.de/han/caj/oversea.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&filename=ZXXY201828006 | ||