底泥中的十溴二苯乙烷的短期暴露对斑马鱼胚胎和幼鱼神经毒性的研究（英文） : = Neurological responses of embryo-larval zebrafish to short-term sediment exposure to decabromodiphenylethane
目的:评估底泥中的十溴二苯乙烷(DBDPE)对斑马鱼早期发育阶段的胚胎毒性和神经行为毒性,并探索其潜在影响机制。创新点:底泥暴露能更真实地反应DBDPE等强疏水性污染物在实际环境中的暴露情况,有利于污染物生态风险评估的科学性和准确性。方法:将受精后4小时(4 hpf)的斑马鱼胚胎置于对照底泥和染毒底泥(DBDPE系列浓度)中进行短期暴露,观察不同发育阶段的存活率、孵化率、畸形率以及行为(包括触碰反应和自由泳动)效应;并通过斑马鱼幼鱼的乙酰胆碱酶活性、神经系统的相关基因(α1-tubulin和gap43)的转录水平以及斑马鱼整体组织的细胞凋亡情况的检测探讨其神经毒性的潜在机制。结论:DBDPE从4 hpf处理至120 hpf,各浓度组的斑马鱼均未出现明显的畸形和死亡。在72 hpf时,最低浓度组(62.5μg/kg)DBDPE轻微加快了斑马鱼的孵化,而最高浓度组(1000.0μg/kg)DBDPE轻微延迟斑马鱼的孵化。所有浓度组的DBDPE对48 hpf时斑马鱼的触碰反应没有任何影响,最高浓度组(1000.0μg/kg)DBDPE对120 hpf时斑马鱼的自由泳动总距离有显著的抑制作用(P<0.05)。但是,斑马鱼的乙酰胆碱酶活性、α1-tubulin和gap43的转录水平未发生显著变化,所有浓度组的DBDPE亦均未诱发斑马鱼整体组织的细胞凋亡。.
Decabromodiphenylethane(DBDPE) has been widely used as an alternative flame retardant due to the restriction or phase-out of traditional polybrominated diphenyl ethers(PBDEs), and is of increasing concern regarding its ubiquity, persistence, and potential adverse effects. In the present study, the toxicological effects of DBDPE were evaluated using zebrafish as an in vivo model. Upon being exposed to DBDPE-polluted sediments for a short term, it was found that the mortality and malformation of zebrafish(including edema, bent notochord, and bent tail) were not affected even at the highest concentration tested(1000.0 μg/kg dry sediment). Regarding behavioral responses, it was found that zebrafish larvae of 48 hours post fertilization(hpf) in all groups escaped successfully with a touch to the dorsal fin. However, when exposed to the highest DBDPE concentration, the larvae of 120 hpf exhibited significantly smaller distances as compared to the control. Moreover, the results of the acetylcholinesterase(AChE) activity, the expression levels of two important nerve-related genes, and the cell apoptosis all indicated that DBDPE posed low neurotoxicity in embryo-larval zebrafish. The results in this study shed some light on the potential risks of DBDPE in the real environment and highlight the application of the sediment exposure route in the future..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018-05-03 2018 |
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| Erschienen: |
2018-05-03 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2018 |
|---|---|
| Enthalten in: |
Journal of Zhejiang University / Science B - (2018), 05 vom: 03. Mai, Seite 400-408 Original Letters: Enthalten in (DE-576)281318174 (DE-576)281318174 |
| Reihe: |
China Academic Journals (CAJ), A, 理工A(数学物理力学天地生) = Mathematics/ Physics/ Mechanics/ Astronomy |
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| Sprache: |
Chinesisch |
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| Weiterer Titel: |
Neurological responses of embryo-larval zebrafish to short-term sediment exposure to decabromodiphenylethane |
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| Beteiligte Personen: |
Mei-qing JIN [VerfasserIn] |
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| Links: |
oversea.cnki.net [lizenzpflichtig] |
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| Anmerkungen: |
Author info:Mei-qing JIN;Dong ZHANG;Ying ZHANG;Shan-shan ZHOU;Xian-ting LU;Hong-ting ZHAO;College of Materials Science and Environmental Engineering, Hangzhou Dianzi University;Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Science,East China Normal University;College of Environment, Zhejiang University of Technology |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
CAJ593376536 |
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| 245 | 1 | 0 | |a 底泥中的十溴二苯乙烷的短期暴露对斑马鱼胚胎和幼鱼神经毒性的研究（英文） |b = Neurological responses of embryo-larval zebrafish to short-term sediment exposure to decabromodiphenylethane |
| 246 | 3 | 1 | |a Neurological responses of embryo-larval zebrafish to short-term sediment exposure to decabromodiphenylethane |
| 264 | 1 | |c 2018-05-03 | |
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| 500 | |a Author info:Mei-qing JIN;Dong ZHANG;Ying ZHANG;Shan-shan ZHOU;Xian-ting LU;Hong-ting ZHAO;College of Materials Science and Environmental Engineering, Hangzhou Dianzi University;Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Science,East China Normal University;College of Environment, Zhejiang University of Technology | ||
| 520 | |a 目的:评估底泥中的十溴二苯乙烷(DBDPE)对斑马鱼早期发育阶段的胚胎毒性和神经行为毒性,并探索其潜在影响机制。创新点:底泥暴露能更真实地反应DBDPE等强疏水性污染物在实际环境中的暴露情况,有利于污染物生态风险评估的科学性和准确性。方法:将受精后4小时(4 hpf)的斑马鱼胚胎置于对照底泥和染毒底泥(DBDPE系列浓度)中进行短期暴露,观察不同发育阶段的存活率、孵化率、畸形率以及行为(包括触碰反应和自由泳动)效应;并通过斑马鱼幼鱼的乙酰胆碱酶活性、神经系统的相关基因(α1-tubulin和gap43)的转录水平以及斑马鱼整体组织的细胞凋亡情况的检测探讨其神经毒性的潜在机制。结论:DBDPE从4 hpf处理至120 hpf,各浓度组的斑马鱼均未出现明显的畸形和死亡。在72 hpf时,最低浓度组(62.5μg/kg)DBDPE轻微加快了斑马鱼的孵化,而最高浓度组(1000.0μg/kg)DBDPE轻微延迟斑马鱼的孵化。所有浓度组的DBDPE对48 hpf时斑马鱼的触碰反应没有任何影响,最高浓度组(1000.0μg/kg)DBDPE对120 hpf时斑马鱼的自由泳动总距离有显著的抑制作用(P<0.05)。但是,斑马鱼的乙酰胆碱酶活性、α1-tubulin和gap43的转录水平未发生显著变化,所有浓度组的DBDPE亦均未诱发斑马鱼整体组织的细胞凋亡。 | ||
| 520 | |a Decabromodiphenylethane(DBDPE) has been widely used as an alternative flame retardant due to the restriction or phase-out of traditional polybrominated diphenyl ethers(PBDEs), and is of increasing concern regarding its ubiquity, persistence, and potential adverse effects. In the present study, the toxicological effects of DBDPE were evaluated using zebrafish as an in vivo model. Upon being exposed to DBDPE-polluted sediments for a short term, it was found that the mortality and malformation of zebrafish(including edema, bent notochord, and bent tail) were not affected even at the highest concentration tested(1000.0 μg/kg dry sediment). Regarding behavioral responses, it was found that zebrafish larvae of 48 hours post fertilization(hpf) in all groups escaped successfully with a touch to the dorsal fin. However, when exposed to the highest DBDPE concentration, the larvae of 120 hpf exhibited significantly smaller distances as compared to the control. Moreover, the results of the acetylcholinesterase(AChE) activity, the expression levels of two important nerve-related genes, and the cell apoptosis all indicated that DBDPE posed low neurotoxicity in embryo-larval zebrafish. The results in this study shed some light on the potential risks of DBDPE in the real environment and highlight the application of the sediment exposure route in the future. | ||
| 610 | 2 | 4 | |a College of Materials Science and Environmental Engineering, Hangzhou Dianzi University |
| 610 | 2 | 4 | |a Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Science,East China Normal University |
| 610 | 2 | 4 | |a College of Environment, Zhejiang University of Technology |
| 650 | 4 | |a 环境生物学 | |
| 650 | 4 | |a 环境科学基础理论 | |
| 650 | 4 | |a 环境科学、安全科学 | |
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| 650 | 4 | |a Chemistry/ Metallurgy/ Environment/ Mine Industry | |
| 650 | 4 | |a 十溴二苯乙烷 | |
| 650 | 4 | |a 阻燃剂 | |
| 650 | 4 | |a 斑马鱼 | |
| 650 | 4 | |a 神经毒性 | |
| 650 | 4 | |a Decabromodiphenylethane | |
| 650 | 4 | |a Flame retardant | |
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| 700 | 0 | |a Shan-shan ZHOU |4 oth | |
| 700 | 0 | |a Xian-ting LU |4 oth | |
| 700 | 0 | |a Hong-ting ZHAO |4 oth | |
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