草莓镶脉病毒(SVBV)ORFⅠ基因的克隆及序列分析 : = Cloning and Sequence Analysis of Gene ORFⅠ of Strawberry vein banding virus(SVBV)
用CTAB法从感染草莓镶脉病毒(SVBV)的草莓叶片中提取总DNA,设计特异性引物扩增含有SVBVORFⅠ的片段,克隆并测序。序列分析表明,该片段全长1 033个核苷酸,其中包含的ORFⅠ全长987个核苷酸,编码328个氨基酸。结果表明:将其与花椰菜花叶病毒属其他成员的ORFⅠ序列相比较,中国SVBVORFⅠ与美国SVBV ORFⅠ序列相似性最高,达88.3%。进一步构建SVBV及其同属其他成员ORFⅠ的系统关系树,结果显示:中国SVBV ORFⅠ与美国SVBV ORFⅠ单独形成1个亚分支,说明来源于草莓的2个SVBV亲缘关系最近。.
The total DNA was extracted from strawberry leaves infected with SVBV by CTAB method.Specific primer pair was designed to amplify the fragment including SVBV ORFⅠ,and then the fragment was cloned and sequenced.Sequence analysis results showed that the fragment was compzosed of 1 033 nts,including 987 nts of full-length ORFⅠgene encoding 328 amino acids.Comparing the sequence of SVBV ORFⅠwith that of the ORFⅠof other members of Caulimovirus,the result showed that Chinese SVBV ORFⅠshared the highest sequence similarity(88.3%) with that of the American SVBV.A phylogenetic tree based on alignment of the nucleotide sequences of ORF Ⅰof SVBV and other members of Caulimovirus was constructed.The result indicated that the Chinese SVBV ORFⅠand the American SVBV ORFⅠclustered into a separate subgroup.It illustrated that the two SVBV derived from strawberry had the closest relationship..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2010-11-15 14:51 2012 |
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| Erschienen: |
2010-11-15 14:51 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2012 |
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| Enthalten in: |
Ji lin nong ye da xue xue bao - (2012), 01 vom: 15 14:51. Nov., Seite 62-65 Original Letters: Enthalten in 吉林农业大学学报 (DE-600)2989818-3 (DE-600)2989818-3 吉林省长春市 |
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| Sprache: |
Chinesisch |
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| Weiterer Titel: |
Cloning and Sequence Analysis of Gene ORFⅠ of Strawberry vein banding virus(SVBV) |
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| Beteiligte Personen: |
贾琳 [VerfasserIn] |
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| Links: |
oversea.cnki.net [lizenzpflichtig] |
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| Themen: |
农业科学 |
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| Anmerkungen: |
Author info:JIA Lin,NI Fang-rui,LI Rui,JIANG Lei,DING Fei,JIANG TongSchool of Plant Protection,Anhui Agricultural University,Hefei 230036,China |
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| 245 | 1 | 0 | |a 草莓镶脉病毒(SVBV)ORFⅠ基因的克隆及序列分析 |b = Cloning and Sequence Analysis of Gene ORFⅠ of Strawberry vein banding virus(SVBV) |
| 246 | 3 | 1 | |a Cloning and Sequence Analysis of Gene ORFⅠ of Strawberry vein banding virus(SVBV) |
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| 520 | |a 用CTAB法从感染草莓镶脉病毒(SVBV)的草莓叶片中提取总DNA,设计特异性引物扩增含有SVBVORFⅠ的片段,克隆并测序。序列分析表明,该片段全长1 033个核苷酸,其中包含的ORFⅠ全长987个核苷酸,编码328个氨基酸。结果表明:将其与花椰菜花叶病毒属其他成员的ORFⅠ序列相比较,中国SVBVORFⅠ与美国SVBV ORFⅠ序列相似性最高,达88.3%。进一步构建SVBV及其同属其他成员ORFⅠ的系统关系树,结果显示:中国SVBV ORFⅠ与美国SVBV ORFⅠ单独形成1个亚分支,说明来源于草莓的2个SVBV亲缘关系最近。 | ||
| 520 | |a The total DNA was extracted from strawberry leaves infected with SVBV by CTAB method.Specific primer pair was designed to amplify the fragment including SVBV ORFⅠ,and then the fragment was cloned and sequenced.Sequence analysis results showed that the fragment was compzosed of 1 033 nts,including 987 nts of full-length ORFⅠgene encoding 328 amino acids.Comparing the sequence of SVBV ORFⅠwith that of the ORFⅠof other members of Caulimovirus,the result showed that Chinese SVBV ORFⅠshared the highest sequence similarity(88.3%) with that of the American SVBV.A phylogenetic tree based on alignment of the nucleotide sequences of ORF Ⅰof SVBV and other members of Caulimovirus was constructed.The result indicated that the Chinese SVBV ORFⅠand the American SVBV ORFⅠclustered into a separate subgroup.It illustrated that the two SVBV derived from strawberry had the closest relationship. | ||
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