Embryoid body formation from embryonic and induced pluripotent stem cells: Benefits of bioreactors : = Embryoid body formation from embryonic and induced pluripotent stem cells:Benefits of bioreactors
Embryonic stem(ES)cells have the ability to differ-entiate into all germ layers,holding great promise not only for a model of early embryonic development but also for a robust cell source for cell-replacement therapies and for drug screening.Embryoid body (EB)formation from ES cells is a common method for producing different cell lineages for further applications. However,conventional techniques such as hanging drop or static suspension culture are either inherently incapable of large scale production or exhibit limited control over cell aggregation during EB formation and subsequent EB aggregation.For standardized mass EB production,a well defined scale-up platform is necessary.Recently,novel scenario methods of EB formation in hydrodynamic conditions created by bioreactor culture systems using stirred suspension systems(spinner flasks),rotating cell culture system and rotary orbital culture have allowed large-scale EB formation.Their use allows for continuous monitoring and control of the physical and chemical environment which is difficult to achieve by traditional methods.This review summarizes the current state of production of EBs derived from pluripotent cells in various culture systems.Furthermore,an overview of high quality EB formation strategies coupled with systems for in vitro differentiation into various cell types to be applied in cell replacement therapy is provided in this review. Recently,new insights in induced pluripotent stem(iPS) cell technology showed that differentiation and lineage commitment are not irreversible processes and this has opened new avenues in stem cell research.These cells are equivalent to ES cells in terms of both self-renewal and differentiation capacity.Hence,culture systems for expansion and differentiation of iPS cells can also apply methodologies developed with ES cells,although direct evidence of their use for iPS cells is still limited..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009-12-31 2009 |
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| Erschienen: |
2009-12-31 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2009 |
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| Enthalten in: |
World journal of stem cells - (2009), 01 vom: 31. Dez., Seite 11-21 Original Letters: Enthalten in (DE-576)334342295 (DE-576)334342295 |
| Reihe: |
China Academic Journals (CAJ), E, 医药卫生科技 = Medicine & Public Health |
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| Sprache: |
Chinesisch |
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| Weiterer Titel: |
Embryoid body formation from embryonic and induced pluripotent stem cells:Benefits of bioreactors |
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| Beteiligte Personen: |
Sasitorn Rungarunlert [VerfasserIn] |
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| Links: |
oversea.cnki.net [lizenzpflichtig] |
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| Anmerkungen: |
Author info:Sasitorn Rungarunlert,Agricultural Biotechnology Center, H-2100 Gdll,Hungary Sasitorn Rungarunlert,Andras Dinnyes,Molecular Animal Biotechnology Laboratory,Szent Istvan University,H-2100 Gdll,Hungary Sasitorn Rungarunlert,Mongkol Techakumphu,Department of Obstetrics,Gynaecology and Reproduction,Faculty of Veterinary Science,Chulalongkorn University,Bangkok 10330,Thailand Melinda K Pirity,Andras Dinnyes,BioTalentum Ltd.,Aulich Lajos u.26.H-2100,Gdll,Hungary ,DSc,Professor,Series Editor |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
CAJ325003459 |
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| 500 | |a Author info:Sasitorn Rungarunlert,Agricultural Biotechnology Center, H-2100 Gdll,Hungary Sasitorn Rungarunlert,Andras Dinnyes,Molecular Animal Biotechnology Laboratory,Szent Istvan University,H-2100 Gdll,Hungary Sasitorn Rungarunlert,Mongkol Techakumphu,Department of Obstetrics,Gynaecology and Reproduction,Faculty of Veterinary Science,Chulalongkorn University,Bangkok 10330,Thailand Melinda K Pirity,Andras Dinnyes,BioTalentum Ltd.,Aulich Lajos u.26.H-2100,Gdll,Hungary ,DSc,Professor,Series Editor | ||
| 520 | |a Embryonic stem(ES)cells have the ability to differ-entiate into all germ layers,holding great promise not only for a model of early embryonic development but also for a robust cell source for cell-replacement therapies and for drug screening.Embryoid body (EB)formation from ES cells is a common method for producing different cell lineages for further applications. However,conventional techniques such as hanging drop or static suspension culture are either inherently incapable of large scale production or exhibit limited control over cell aggregation during EB formation and subsequent EB aggregation.For standardized mass EB production,a well defined scale-up platform is necessary.Recently,novel scenario methods of EB formation in hydrodynamic conditions created by bioreactor culture systems using stirred suspension systems(spinner flasks),rotating cell culture system and rotary orbital culture have allowed large-scale EB formation.Their use allows for continuous monitoring and control of the physical and chemical environment which is difficult to achieve by traditional methods.This review summarizes the current state of production of EBs derived from pluripotent cells in various culture systems.Furthermore,an overview of high quality EB formation strategies coupled with systems for in vitro differentiation into various cell types to be applied in cell replacement therapy is provided in this review. Recently,new insights in induced pluripotent stem(iPS) cell technology showed that differentiation and lineage commitment are not irreversible processes and this has opened new avenues in stem cell research.These cells are equivalent to ES cells in terms of both self-renewal and differentiation capacity.Hence,culture systems for expansion and differentiation of iPS cells can also apply methodologies developed with ES cells,although direct evidence of their use for iPS cells is still limited. | ||
| 610 | 2 | 4 | |a Agricultural Biotechnology Center, H-2100 Gdll,Hungary |
| 610 | 2 | 4 | |a Andras Dinnyes,Molecular Animal Biotechnology Laboratory,Szent Istvan University,H-2100 Gdll,Hungary |
| 610 | 2 | 4 | |a Department of Obstetrics,Gynaecology and Reproduction,Faculty of Veterinary Science,Chulalongkorn University,Bangkok 10330,Thailand |
| 610 | 2 | 4 | |a BioTalentum Ltd.,Aulich Lajos u.26.H-2100,Gdll,Hungary |
| 650 | 4 | |a 人体形态学 | |
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