复合PCR鉴定巴氏杆菌和副猪嗜血杆菌的方法 : = Method of Multiplex-PCR for Identification of Pasteurella multocida and Haemophilus parasuis
在已经建立的多杀性巴氏杆菌、副猪嗜血杆菌的单项PCR诊断方法的基础上,通过对扩增条件和引物的筛选,成功地建立了PM、HPS复合PCR诊断方法,利用一次PCR反应,即可同时扩增多杀性巴氏杆菌的457 bp和副猪嗜血杆菌的1 090 bp的特异性片段。检测灵敏度分别达7.2 pg DNA/1 040 CFU和16.0 pg DNA/2 300CFU,用所建方法检测临床分离的23株可疑菌株和本实验室人工感染猪分离菌,结果显示,23株可疑株有2株检出多杀性巴氏杆菌特异性条带,没有检出副猪嗜血杆菌特异性条带;人工感染分离菌株均扩增出副猪嗜血杆菌特异性条带。表明该方法能够对临床上这2种疾病进行鉴别诊断。.
Multiplex-PCR detection method for Pasteurella multocida(PM) and Haemophilus parasuis(HPS) had been developed based on published single PCR for these pathogens.The PCR reagents,annealing temperature and the PCR procedure were optimized and the primers were screened,the PCR products including 457 bp of PM and 1 090 bp of HPS were obtained just by one test.The detectable minimum dose of HPS was 1.04×10~3 CFU or 7.2 pg DNA and PM was 2.3×10~3 CFU or 16 pg DNA.Among 23 suspected isolates,two strains showed PM fragments by multiplex-PCR method, no HPS fragments,but all artificially infected isolates showed HPS fragments.The results suggested that the multiplex-PCR method could be used in clinical diagnosis of PM and HPS..
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2009-12-31 2009 |
|---|---|
| Erschienen: |
2009-12-31 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2009 |
|---|---|
| Enthalten in: |
Jiang su nong ye xue bao - (2009), 06 vom: 31. Dez., Seite 1315-1319 Original Letters: Enthalten in 江苏农业学报 (DE-600)2993832-6 (DE-600)2993832-6 江苏省南京市 |
| Reihe: |
|---|
| Sprache: |
Chinesisch |
|---|
| Weiterer Titel: |
Method of Multiplex-PCR for Identification of Pasteurella multocida and Haemophilus parasuis |
|---|
| Beteiligte Personen: |
贺英 [VerfasserIn] |
|---|
| Links: |
oversea.cnki.net [lizenzpflichtig] |
|---|
| Themen: |
中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部草食动物疫病重点开放实验室,农业部畜禽病毒学重点开放实验室 |
|---|
| Anmerkungen: |
Author info:HE Ying,ZHAO Ping,CHU Yue-feng,GAO Peng-cheng,ZHANG Jian-jun,LU Zhong-xin (Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture,Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou 730046,China) |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
CAJ322842247 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | CAJ322842247 | ||
| 003 | DE-627 | ||
| 005 | 20221206201355.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 221206s2009 cc |||||o 00| ||chi c | ||
| 035 | |a (DE-627)CAJ322842247 | ||
| 035 | |a (SBB-XA)CAJ_JSNB200906027 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a chi | ||
| 044 | |c XB-CN | ||
| 084 | |a ASIEN |q DE-1a |2 fid | ||
| 084 | |a S858.28 |2 clc | ||
| 100 | 0 | |a 贺英 |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a 复合PCR鉴定巴氏杆菌和副猪嗜血杆菌的方法 |b = Method of Multiplex-PCR for Identification of Pasteurella multocida and Haemophilus parasuis |
| 246 | 3 | 1 | |a Method of Multiplex-PCR for Identification of Pasteurella multocida and Haemophilus parasuis |
| 264 | 1 | |c 2009-12-31 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 490 | 0 | |a China Academic Journals (CAJ) |a D |a 农业科技 = Agriculture | |
| 500 | |a Author info:HE Ying,ZHAO Ping,CHU Yue-feng,GAO Peng-cheng,ZHANG Jian-jun,LU Zhong-xin (Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture,Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou 730046,China) | ||
| 520 | |a 在已经建立的多杀性巴氏杆菌、副猪嗜血杆菌的单项PCR诊断方法的基础上,通过对扩增条件和引物的筛选,成功地建立了PM、HPS复合PCR诊断方法,利用一次PCR反应,即可同时扩增多杀性巴氏杆菌的457 bp和副猪嗜血杆菌的1 090 bp的特异性片段。检测灵敏度分别达7.2 pg DNA/1 040 CFU和16.0 pg DNA/2 300CFU,用所建方法检测临床分离的23株可疑菌株和本实验室人工感染猪分离菌,结果显示,23株可疑株有2株检出多杀性巴氏杆菌特异性条带,没有检出副猪嗜血杆菌特异性条带;人工感染分离菌株均扩增出副猪嗜血杆菌特异性条带。表明该方法能够对临床上这2种疾病进行鉴别诊断。 | ||
| 520 | |a Multiplex-PCR detection method for Pasteurella multocida(PM) and Haemophilus parasuis(HPS) had been developed based on published single PCR for these pathogens.The PCR reagents,annealing temperature and the PCR procedure were optimized and the primers were screened,the PCR products including 457 bp of PM and 1 090 bp of HPS were obtained just by one test.The detectable minimum dose of HPS was 1.04×10~3 CFU or 7.2 pg DNA and PM was 2.3×10~3 CFU or 16 pg DNA.Among 23 suspected isolates,two strains showed PM fragments by multiplex-PCR method, no HPS fragments,but all artificially infected isolates showed HPS fragments.The results suggested that the multiplex-PCR method could be used in clinical diagnosis of PM and HPS. | ||
| 610 | 2 | 4 | |a 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部草食动物疫病重点开放实验室,农业部畜禽病毒学重点开放实验室 |
| 650 | 4 | |a 动物医学（兽医学） | |
| 650 | 4 | |a 畜牧、动物医学、狩猎、蚕、蜂 | |
| 650 | 4 | |a 农业科学 | |
| 650 | 4 | |a Animal Husbandry and Veterinary | |
| 650 | 4 | |a 农业科技 | |
| 650 | 4 | |a Agriculture | |
| 700 | 0 | |a 赵萍 |4 oth | |
| 700 | 0 | |a 储岳峰 |4 oth | |
| 700 | 0 | |a 高鹏程 |4 oth | |
| 700 | 0 | |a 张建军 |4 oth | |
| 700 | 0 | |a 逯忠新 |4 oth | |
| 773 | 0 | 8 | |6 880-01 |i Enthalten in |t Jiang su nong ye xue bao |d Jiang su sheng nan jing shi, 1985 |g (2009), 06 vom: 31. Dez., Seite 1315-1319 |h Online-Ressource |w (DE-627)CAJ067661963 |w (DE-600)2993832-6 |7 nnns |
| 773 | 1 | 8 | |g year:2009 |g number:06 |g day:31 |g month:12 |g pages:1315-1319 |
| 856 | 4 | 0 | |u http://erf.sbb.spk-berlin.de/han/caj/oversea.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&filename=JSNB200906027 |x Verlag |y CrossAsia Link |z Deutschlandweit zugänglich |
| 856 | 4 | 0 | |u https://oversea.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&filename=JSNB200906027 |x Verlag |z lizenzpflichtig |
| 880 | 0 | 8 | |6 773-01/Hans |i Enthalten in |t 江苏农业学报 |d 江苏省南京市 |h Online-Ressource |w (DE-627)CAJ067661963 |w (DE-600)2993832-6 |7 nnns |
| 912 | |a ZDB-1-CAJ | ||
| 912 | |a GBV_NL_CAJ | ||
| 912 | |a FID-ASIEN | ||
| 912 | |a SSG-OLC-OA3.1 | ||
| 912 | |a SSG-OLC-CAJ | ||
| 936 | u | w | |j 2009 |e 06 |b 31 |c 12 |h 1315-1319 |
| 951 | |a AR | ||
| 952 | |j 2009 |e 06 |b 31 |c 12 |h 1315-1319 | ||
| 980 | |2 11 |1 01 |x 0001 |b 330023594 |c 00 |f 5:FIDXASIA |d --%%-- |e --%%-- |j --%%-- |y k |z 06-12-22 | ||
| 981 | |2 11 |1 01 |x 0001 |r http://erf.sbb.spk-berlin.de/han/caj/oversea.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&filename=JSNB200906027 | ||