SARS冠状病毒棘突蛋白的S1蛋白诱导小鼠产生中和抗体的研究 : = Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain
Objective To study the antigenicity of SARS associated coronavirus (CoV) spike S1 (12-672Aa) domain Methods BALB/c mice were immunized with a plasmid bearing codon-optimized SARS-CoV (Tor2 strain) S1 domain and then boosted with purified S1 protein; the SARS-CoV specific IgG antibody was tested by ELISA and neutralization antibody was determined by in vitro microneutralization assay Results S1 domain of SARS-CoV spike, which has been demonstrated harboring the receptor binding domain, successfully elicited SARS-CoV specific IgG antibody in mouse after combined immunization with DNA and purified S1 protein; the antibody elicited solely by S1 could potently neutralize SARS-CoV (HKU-39849) in vitro, 50% of 1 000 TCID 50 SARS-CoV challenged cells were protected from viral infection by a 1∶1 49968 dilution of mice sera immunized with S1 protein, but negative control sera showed no protection Conclusion S1 domain of SARS-CoV spike protein, which is responsible for receptor binding, can efficiently and sufficiently induce highly potent neutralizing antibody in mice This result suggested that S1 domain could be an effective subunit vaccines against SARS-CoV.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2004-09-30 2004 |
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| Erschienen: |
2004-09-30 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2004 |
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| Enthalten in: |
Zhong hua shi yan he lin chuang bing du xue za zhi - (2004), 3 vom: 30. Sept., Seite 59-61 Original Letters: Enthalten in 中华实验和临床病毒学杂志 (DE-600)2997101-9 (DE-600)2997101-9 北京市 |
| Reihe: |
China Academic Journals (CAJ), E, 医药卫生科技 = Medicine & Public Health China Academic Journals (CAJ), A, 理工A(数学物理力学天地生) = Mathematics/ Physics/ Mechanics/ Astronomy |
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| Sprache: |
Chinesisch |
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| Weiterer Titel: |
Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain |
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| Beteiligte Personen: |
张云 [VerfasserIn] |
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| Links: |
oversea.cnki.net [lizenzpflichtig] |
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| Umfang: |
3 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
CAJ231203322 |
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| 245 | 1 | 0 | |a SARS冠状病毒棘突蛋白的S1蛋白诱导小鼠产生中和抗体的研究 |b = Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain |
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| 520 | |a Objective To study the antigenicity of SARS associated coronavirus (CoV) spike S1 (12-672Aa) domain Methods BALB/c mice were immunized with a plasmid bearing codon-optimized SARS-CoV (Tor2 strain) S1 domain and then boosted with purified S1 protein; the SARS-CoV specific IgG antibody was tested by ELISA and neutralization antibody was determined by in vitro microneutralization assay Results S1 domain of SARS-CoV spike, which has been demonstrated harboring the receptor binding domain, successfully elicited SARS-CoV specific IgG antibody in mouse after combined immunization with DNA and purified S1 protein; the antibody elicited solely by S1 could potently neutralize SARS-CoV (HKU-39849) in vitro, 50% of 1 000 TCID 50 SARS-CoV challenged cells were protected from viral infection by a 1∶1 49968 dilution of mice sera immunized with S1 protein, but negative control sera showed no protection Conclusion S1 domain of SARS-CoV spike protein, which is responsible for receptor binding, can efficiently and sufficiently induce highly potent neutralizing antibody in mice This result suggested that S1 domain could be an effective subunit vaccines against SARS-CoV | ||
| 610 | 2 | 4 | |a 中国医学科学院基础医学研究所中国协和医科大学基础医学院病原室 |
| 610 | 2 | 4 | |a 中国医学科学院血液病研究所 |
| 610 | 2 | 4 | |a 美国哈佛大学肿瘤研究所 |
| 610 | 2 | 4 | |a 中国医学科学院实验动物研究所 |
| 610 | 2 | 4 | |a 香港大学微生物系 |
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