Toxicity of water-soluble D-g-PNIPAM polymers in a complex with chemotherapy drugs and mechanism of their action in vitro / Svitlana Prylutska, Anna Grebinyk, Stanislav Ponomarenko, Defne Gövem, Vasyl Chumachenko, Nataliya Kutsevol, Mykola Petrovsky, Uwe Ritter, Marcus Frohme, Jacek Piosik and Yuriy Prylutskyy
The application of a biocompatible polymer nanocarrier can provide target delivery to tumor tissues, improved pharmacokinetics, controlled drug release, etc. Therefore, the proposed strategy was to use the water-soluble star-like copolymers with a Dextran core and Poly(N-isopropylacrylamide) grafts (D-g-PNIPAM) for conjugation with the widely used chemotherapy drugs in oncology-Cisplatin (Cis-Pt) and Doxorubicin (Dox). The molecular characteristics of the copolymer were received using size-exclusion chromatography. The physicochemical characterization of the D-g-PNIPAM-Cis-Pt (or Dox) nanosystem was conducted using dynamic light scattering and FTIR spectroscopy. Using traditional biochemical methods, a comparative analysis of the enhancement of the cytotoxic effect of free Cis-Pt and Dox in combination with D-g-PNIPAM copolymers was performed in cancer cells of the Lewis lung carcinoma line, which are both sensitive and resistant to Dox; in addition, the mechanism of their action in vitro was evaluated..
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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Enthalten in: |
International journal of molecular sciences - 25(2024), 5, Artikel-ID 3069, Seite 1-15 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Prylutska, Svitlana [VerfasserIn] |
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Links: |
Volltext [kostenfrei] |
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doi: |
10.3390/ijms25053069 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
1885470436 |
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