In vivo characterization of the novel ebolavirus Bombali virus suggests a low pathogenic potential for humans / B.S. Bodmer, A. Breithaupt, M. Heung, J.E. Brunetti, C. Henkel, J. Müller-Guhl, E. Rodríguez, L. Wendt, S.L. Winter, M. Vallbracht, A. Müller, S. Römer, P. Chlanda, C. Muñoz-Fontela, T. Hoenen and B. Escudero-Pérez

Ebolaviruses cause outbreaks of haemorrhagic fever in Central and West Africa. Some members of this genus such as Ebola virus (EBOV) are highly pathogenic, with case fatality rates of up to 90%, whereas others such as Reston virus (RESTV) are apathogenic for humans. Bombali virus (BOMV) is a novel ebolavirus for which complete genome sequences were recently found in free-tailed bats, although no infectious virus could be isolated. Its pathogenic potential for humans is unknown. To address this question, we first determined whether proteins encoded by the available BOMV sequence found in Chaerephon pumilus were functional in in vitro assays. The correction of an apparent sequencing error in the glycoprotein based on these data then allowed us to generate infectious BOMV using reverse genetics and characterize its infection of human cells. Furthermore, we used HLA-A2-transgenic, NOD-scid-IL-2γ receptor-knockout (NSG-A2) mice reconstituted with human haematopoiesis as a model to evaluate the pathogenicity of BOMV in vivo in a human-like immune environment. These data demonstrate that not only does BOMV show a slower growth rate than EBOV in vitro, but it also shows low pathogenicity in humanized mice, comparable to previous studies using RESTV. Taken together, these findings suggest a low pathogenic potential of BOMV for humans..

Medienart:

E-Artikel

Erscheinungsjahr:

18 Jan 2023

2023

Erschienen:

18 Jan 2023

Enthalten in:

Zur Gesamtaufnahme - volume:12

Enthalten in:

Emerging Microbes & Infections - 12(2023), Artikel-ID 2164216, Seite 1-12

Sprache:

Englisch

Beteiligte Personen:

Bodmer, Bianca Susanne, 1990- [VerfasserIn]
Breithaupt, A. [VerfasserIn]
Heung, M. [VerfasserIn]
Brunetti, J. E. [VerfasserIn]
Henkel, C. [VerfasserIn]
Müller-Guhl, J. [VerfasserIn]
Rodríguez, E. [VerfasserIn]
Wendt, L. [VerfasserIn]
Winter, Sophie L., 1992- [VerfasserIn]
Vallbracht, Melina [VerfasserIn]
Müller, A. [VerfasserIn]
Römer, S. [VerfasserIn]
Chlanda, Petr [VerfasserIn]
Muñoz-Fontela, C. [VerfasserIn]
Hoenen, T. [VerfasserIn]
Escudero-Pérez, B. [VerfasserIn]

Links:

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Themen:

Bombali virus
Ebolavirus
Filovirus
Full-length clone
Humanized mice
Pathogenic potential
Reverse genetics
Virulence

Anmerkungen:

Gesehen am 15.11.2023

Umfang:

Illustrationen

12

doi:

10.1080/22221751.2022.2164216

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

1838930809

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