Details der Publikation - Structural organization of a conserved gene cluster of Tupaia herpesvirus encoding the DNA polymerase, glycoprotein B, a probable processing and transport protein, and the major DNA binding protein

Structural organization of a conserved gene cluster of Tupaia herpesvirus encoding the DNA polymerase, glycoprotein B, a probable processing and transport protein, and the major DNA binding protein / Udo Bahr, Christoph Springfeld, Christian A. Tidona, Gholamreza Darai

The Tupaia herpesviruses (THVs) have been isolated from malignant lymphoma tissue cultures and from degenerating lung and spleen cell cultures of tree shrews (Tupaia spp.). Recently we succeeded in the localization of the gene locus of the THV DNA polymerase (DPOL) gene within the viral genome. Based on these results the highly conserved gene cluster of herpesviruses encoding the DPOL, the glycoprotein B (gB), a probable processing and transport protein (PRTP), and the major DNA binding protein (DNBI) was characterized in the genome of THV strain 2 (THV-2) in its entirety. The complete nucleotide sequence of the gene cluster was determined and it was discovered that the THV-2 gene products are most closely related to the corresponding proteins of mammalian cytomegaloviruses. The transcriptional activity of the four genes was confirmed by amplification of a part of the corresponding mRNAs obtained from infected cell RNA by RT-PCR. The homology values and the overall structure of the gene cluster, that shows specific colinearity with the corresponding clusters of the mammalian cytomegaloviruses, is further evidence that THV-2 is a member of the subfamily Betaherpesvirinae..

Medienart:	E-Artikel

Erscheinungsjahr:	28 May 1999 1999
Erschienen:	28 May 1999

Enthalten in:	Zur Gesamtaufnahme - volume:60
Enthalten in:	Virus research - 60(1999), 2, Seite 123-135

Sprache:	Englisch

Beteiligte Personen:	Bahr, Udo [VerfasserIn] Springfeld, Christoph, 1972- [VerfasserIn] Tidona, Christian A., 1971- [VerfasserIn] Darai, Gholamreza, 1940- [VerfasserIn]

Links:	Volltext [lizenzpflichtig] Volltext [lizenzpflichtig]

Themen:	Amino Acid Sequence Animals Carrier Proteins Chromosome Mapping Conserved Sequence DNA, Viral DNA-Binding Proteins DNA-Directed DNA Polymerase Deoxyribonuclease EcoRI Genes, Viral Glycoproteins Herpesviridae Molecular Sequence Data Multigene Family Phylogeny RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic Tupaia Viral Proteins

Anmerkungen:	Gesehen am 19.03.2021

Umfang:	13

doi:	10.1016/s0168-1702(99)00012-x

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	1751859835

Internformat


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245	1	0	\|a Structural organization of a conserved gene cluster of Tupaia herpesvirus encoding the DNA polymerase, glycoprotein B, a probable processing and transport protein, and the major DNA binding protein \|c Udo Bahr, Christoph Springfeld, Christian A. Tidona, Gholamreza Darai
264		1	\|c 28 May 1999
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520			\|a The Tupaia herpesviruses (THVs) have been isolated from malignant lymphoma tissue cultures and from degenerating lung and spleen cell cultures of tree shrews (Tupaia spp.). Recently we succeeded in the localization of the gene locus of the THV DNA polymerase (DPOL) gene within the viral genome. Based on these results the highly conserved gene cluster of herpesviruses encoding the DPOL, the glycoprotein B (gB), a probable processing and transport protein (PRTP), and the major DNA binding protein (DNBI) was characterized in the genome of THV strain 2 (THV-2) in its entirety. The complete nucleotide sequence of the gene cluster was determined and it was discovered that the THV-2 gene products are most closely related to the corresponding proteins of mammalian cytomegaloviruses. The transcriptional activity of the four genes was confirmed by amplification of a part of the corresponding mRNAs obtained from infected cell RNA by RT-PCR. The homology values and the overall structure of the gene cluster, that shows specific colinearity with the corresponding clusters of the mammalian cytomegaloviruses, is further evidence that THV-2 is a member of the subfamily Betaherpesvirinae.
650		4	\|a Amino Acid Sequence
650		4	\|a Animals
650		4	\|a Carrier Proteins
650		4	\|a Chromosome Mapping
650		4	\|a Conserved Sequence
650		4	\|a Deoxyribonuclease EcoRI
650		4	\|a DNA-Binding Proteins
650		4	\|a DNA-Directed DNA Polymerase
650		4	\|a DNA, Viral
650		4	\|a Genes, Viral
650		4	\|a Glycoproteins
650		4	\|a Herpesviridae
650		4	\|a Molecular Sequence Data
650		4	\|a Multigene Family
650		4	\|a Phylogeny
650		4	\|a Reverse Transcriptase Polymerase Chain Reaction
650		4	\|a RNA, Messenger
650		4	\|a Sequence Alignment
650		4	\|a Sequence Analysis, DNA
650		4	\|a Sequence Homology, Amino Acid
650		4	\|a Transcription, Genetic
650		4	\|a Tupaia
650		4	\|a Viral Proteins
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700	1		\|a Tidona, Christian A. \|d 1971- \|e verfasserin \|0 (DE-588)108958654X \|0 (DE-627)853370044 \|0 (DE-576)460024868 \|4 aut
700	1		\|a Darai, Gholamreza \|d 1940- \|e verfasserin \|0 (DE-588)1048550354 \|0 (DE-627)780606256 \|0 (DE-576)402386132 \|4 aut
773	0	8	\|i Enthalten in \|t Virus research \|d Amsterdam [u.a.] : Elsevier Science, 1984 \|g 60(1999), 2, Seite 123-135 \|h Online-Ressource \|w (DE-627)306711222 \|w (DE-600)1500820-4 \|w (DE-576)082436215 \|x 1872-7492 \|7 nnns
773	1	8	\|g volume:60 \|g year:1999 \|g number:2 \|g pages:123-135 \|g extent:13
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912			\|a GBV_USEFLAG_U
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912			\|a GBV_ILN_150
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912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_252
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_374
912			\|a GBV_ILN_602
912			\|a GBV_ILN_647
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2018
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2043
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2158
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2193
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_2807
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4277
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4346
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
912			\|a GBV_ILN_4753
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Structural organization of a conserved gene cluster of Tupaia herpesvirus encoding the DNA polymerase, glycoprotein B, a probable processing and transport protein, and the major DNA binding protein / Udo Bahr, Christoph Springfeld, Christian A. Tidona, Gholamreza Darai

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