Details der Publikation - Resolution of experimental and tick-borne Borrelia burgdorferi infection in mice by passive, but not active immunization using recombinant OspC

Resolution of experimental and tick-borne Borrelia burgdorferi infection in mice by passive, but not active immunization using recombinant OspC / Weimin Zhong, LiseGern, Thomas Stehle, Crisan Museteanu, Michael Kramer, Reinhard Wallich, and Markus M. Simon

Vaccination with outer surface protein A (OspA) of Borrelia burgdorferi prevents subsequent infection and disease in both laboratory animals and humans with high efficacy. OspA-based immunity, however, does not affect established infection due to the loss of OspA expression in the vertebrate host. We show here that repeated passive transfer of mouse and/or rabbit immune sera to recombinant GST-OspC fusion protein resulted in a dose-dependent resolution (1) of fully established arthritis and carditis as well as infection in needle-challenged C.B-17 SCID and (2) of infection in both experimentally and tick-infected BALB/c mice. Unexpectedly, active immunization of disease-susceptible AKR/N mice with GST-OspC only led to prevention but not resolution of disease and infection, in spite of high serum titers of OspC-specific Ab and the expression of ospC in tissue-derived spirochetes. The data suggest that the efficacy of OspC antibody-mediated immunity depends on the immunological history of the recipient and/or environment-dependent regulation of OspC surface expression by spirochetes in vivo. The results encourage further attempts to develop therapeutic vaccination protocols against Lyme disease..

Medienart:	E-Artikel

Erscheinungsjahr:	1999
Erschienen:	1999

Enthalten in:	Zur Gesamtaufnahme - volume:29
Enthalten in:	European journal of immunology - 29(1999), 3, Seite 946-957

Sprache:	Englisch

Beteiligte Personen:	Zhong, Weimin [VerfasserIn] Gern, Lisa [VerfasserIn] Stehle, Thomas [VerfasserIn] Museteanu, Crisan [VerfasserIn] Kramer, Michael D., 1958- [VerfasserIn] Wallich, Reinhard [VerfasserIn] Simon, Markus M. [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Themen:	Animals Antibodies, Bacterial Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Bacterial Vaccines Borrelia burgdorferi Group Disease Models, Animal Female Gene Expression Glutathione Transferase Immunization, Passive Kinetics Lipoproteins Lyme Disease Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, SCID Rabbits Recombinant Fusion Proteins Ticks Vaccination Vaccines, Synthetic

Anmerkungen:	First published: 28 March 2006 Gesehen am 17.02.2021

Umfang:	12

doi:	AID-IMMU946>3.0.CO;2-P

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	1748527584

Internformat


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245	1	0	\|a Resolution of experimental and tick-borne Borrelia burgdorferi infection in mice by passive, but not active immunization using recombinant OspC \|c Weimin Zhong, LiseGern, Thomas Stehle, Crisan Museteanu, Michael Kramer, Reinhard Wallich, and Markus M. Simon
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520			\|a Vaccination with outer surface protein A (OspA) of Borrelia burgdorferi prevents subsequent infection and disease in both laboratory animals and humans with high efficacy. OspA-based immunity, however, does not affect established infection due to the loss of OspA expression in the vertebrate host. We show here that repeated passive transfer of mouse and/or rabbit immune sera to recombinant GST-OspC fusion protein resulted in a dose-dependent resolution (1) of fully established arthritis and carditis as well as infection in needle-challenged C.B-17 SCID and (2) of infection in both experimentally and tick-infected BALB/c mice. Unexpectedly, active immunization of disease-susceptible AKR/N mice with GST-OspC only led to prevention but not resolution of disease and infection, in spite of high serum titers of OspC-specific Ab and the expression of ospC in tissue-derived spirochetes. The data suggest that the efficacy of OspC antibody-mediated immunity depends on the immunological history of the recipient and/or environment-dependent regulation of OspC surface expression by spirochetes in vivo. The results encourage further attempts to develop therapeutic vaccination protocols against Lyme disease.
650		4	\|a Animals
650		4	\|a Antibodies, Bacterial
650		4	\|a Antigens, Bacterial
650		4	\|a Antigens, Surface
650		4	\|a Bacterial Outer Membrane Proteins
650		4	\|a Bacterial Vaccines
650		4	\|a Borrelia burgdorferi Group
650		4	\|a Disease Models, Animal
650		4	\|a Female
650		4	\|a Gene Expression
650		4	\|a Glutathione Transferase
650		4	\|a Immunization, Passive
650		4	\|a Kinetics
650		4	\|a Lipoproteins
650		4	\|a Lyme Disease
650		4	\|a Mice
650		4	\|a Mice, Inbred AKR
650		4	\|a Mice, Inbred BALB C
650		4	\|a Mice, SCID
650		4	\|a Rabbits
650		4	\|a Recombinant Fusion Proteins
650		4	\|a Ticks
650		4	\|a Vaccination
650		4	\|a Vaccines, Synthetic
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700	1		\|a Simon, Markus M. \|e verfasserin \|4 aut
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Resolution of experimental and tick-borne Borrelia burgdorferi infection in mice by passive, but not active immunization using recombinant OspC / Weimin Zhong, LiseGern, Thomas Stehle, Crisan Museteanu, Michael Kramer, Reinhard Wallich, and Markus M. Simon

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