Details der Publikation - Simultaneous detection of circulating and disseminated tumor cells in primary breast cancer patients following neoadjuvant chemotherapy

Simultaneous detection of circulating and disseminated tumor cells in primary breast cancer patients following neoadjuvant chemotherapy / Vincent P. Walter, Florin-Andrei Taran, Markus Wallwiener, Markus Hahn, Sara Y. Brucker, Andreas D. Hartkopf

PURPOSE: Pathological complete response (pCR) is a common endpoint in neoadjuvant chemotherapy (NACT) of primary breast cancer patients (PBC), but does not address the systemic prevalence of minimal residual disease. In this study, we compared pCR with the detection of circulating (CTC) and disseminated tumor cells (DTC) following NACT, as well as their impact on survival. - METHODS: Patients with PBC receiving NACT and consecutive surgery were eligible for this study. CTCs were detected using the CellSearch® system and DTCs were determined using immunocytochemistry (cytokeratin staining with the A45-B/B3 antibody). pCR was defined as ypT0/ypTis and ypN0. - RESULTS: 58 patients were included in the analysis with a median follow-up of 30 months. Of these, 5 (9%) presented with CTCs and 36 (62%) with DTCs. 16 patients (28%) achieved a pCR. No significant correlation between CTCs, DTCs and pCR and no statistically significant impact on disease free (DFS) or overall survival (OS) was apparent. - CONCLUSIONS: Both CTCs and DTCs are detectable after NACT. As we could not show a significant relationship between CTC detection, DTC detection and pCR, all three methods may provide independent information regarding treatment response. Since we were unable to show a significant impact on survival, larger prospective studies that include CTCs and DTCs are needed. These trials should include the molecular characterization of primary tumor tissue, CTCs and DTCs to determine whether these cells are independent subpopulations of malignant cell clones..

Medienart:	E-Artikel

Erscheinungsjahr:	27 January 2018 2018
Erschienen:	27 January 2018

Enthalten in:	Zur Gesamtaufnahme - volume:297
Enthalten in:	Archives of gynecology and obstetrics - 297(2018), 3, Seite 785-790

Sprache:	Englisch

Beteiligte Personen:	Walter, Vincent P. [VerfasserIn] Wallwiener, Markus, 1980- [VerfasserIn]

Links:	Volltext

Themen:	Breast Neoplasms Breast cancer Circulating tumor cells Disseminated tumor cells Female Humans Immunohistochemistry Middle Aged Minimal residual disease Neoadjuvant Therapy Neoadjuvant chemotherapy Neoplasm, Residual Neoplasm Metastasis Neoplastic Cells, Circulating Pathological complete response Prognosis Prospective Studies Reverse Transcriptase Polymerase Chain Reaction

Anmerkungen:	Gesehen am 29.04.2019

Umfang:	6

doi:	10.1007/s00404-018-4669-9

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	1663716668

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245	1	0	\|a Simultaneous detection of circulating and disseminated tumor cells in primary breast cancer patients following neoadjuvant chemotherapy \|c Vincent P. Walter, Florin-Andrei Taran, Markus Wallwiener, Markus Hahn, Sara Y. Brucker, Andreas D. Hartkopf
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520			\|a PURPOSE: Pathological complete response (pCR) is a common endpoint in neoadjuvant chemotherapy (NACT) of primary breast cancer patients (PBC), but does not address the systemic prevalence of minimal residual disease. In this study, we compared pCR with the detection of circulating (CTC) and disseminated tumor cells (DTC) following NACT, as well as their impact on survival. - METHODS: Patients with PBC receiving NACT and consecutive surgery were eligible for this study. CTCs were detected using the CellSearch® system and DTCs were determined using immunocytochemistry (cytokeratin staining with the A45-B/B3 antibody). pCR was defined as ypT0/ypTis and ypN0. - RESULTS: 58 patients were included in the analysis with a median follow-up of 30 months. Of these, 5 (9%) presented with CTCs and 36 (62%) with DTCs. 16 patients (28%) achieved a pCR. No significant correlation between CTCs, DTCs and pCR and no statistically significant impact on disease free (DFS) or overall survival (OS) was apparent. - CONCLUSIONS: Both CTCs and DTCs are detectable after NACT. As we could not show a significant relationship between CTC detection, DTC detection and pCR, all three methods may provide independent information regarding treatment response. Since we were unable to show a significant impact on survival, larger prospective studies that include CTCs and DTCs are needed. These trials should include the molecular characterization of primary tumor tissue, CTCs and DTCs to determine whether these cells are independent subpopulations of malignant cell clones.
650		4	\|a Breast cancer
650		4	\|a Breast Neoplasms
650		4	\|a Circulating tumor cells
650		4	\|a Disseminated tumor cells
650		4	\|a Female
650		4	\|a Humans
650		4	\|a Immunohistochemistry
650		4	\|a Middle Aged
650		4	\|a Minimal residual disease
650		4	\|a Neoadjuvant chemotherapy
650		4	\|a Neoadjuvant Therapy
650		4	\|a Neoplasm Metastasis
650		4	\|a Neoplasm, Residual
650		4	\|a Neoplastic Cells, Circulating
650		4	\|a Pathological complete response
650		4	\|a Prognosis
650		4	\|a Prospective Studies
650		4	\|a Reverse Transcriptase Polymerase Chain Reaction
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Simultaneous detection of circulating and disseminated tumor cells in primary breast cancer patients following neoadjuvant chemotherapy / Vincent P. Walter, Florin-Andrei Taran, Markus Wallwiener, Markus Hahn, Sara Y. Brucker, Andreas D. Hartkopf

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